Liquid Chromatography for Analysis of Metformin in Myrmeleon sp.

Abstract

Myrmeleon sp. is typical of insect larva which has been used in Indonesia for diabetes treatment. However, there is no sufficient scientific report explaining the bioactive compounds in this insect. Based on our preliminary research, this insect contained metformin, i.e. one of bioactive compounds for the treatment of type-2 diabetes. Therefore, this study is focused on the development of separation technique using high-performance liquid chromatography (HPLC) on a reverse phase C-18 column with UV detection to identify and quantify metformin in methanol extract of Myrmeleon sp. Several parameters of HPLC method were optimized in accordance with the high resolution of separation and accurate determination of metformin. Satisfied separation was obtained under gradient elution mode using aqueous methanol/water with gradual increase of methanol from 50-90% and the flow rate of 0.5 mL/min with detection wavelength of 233 nm. The method performed total separation for all compounds in less than 11 min. Spiking technique was chosen for metformin identification and quantitation. Metformin in extract Myrmeleon sp. was eluted at the retention time (t R ) of 4.095 min, similarly with the retention time of standard metformin of 4.092 min. The quantity of metformin in Myrmeleon sp. can be simply determined by comparing the additional area of standard metformin and area of metformin from extract Myrmeleon sp. The results confirmed that the methanol extract of Myrmeleon sp. contained the anti-diabetes compound of metformin of 0.58 mg/g Myrmeleon sp. larvae with acceptable coefficient variation of 5.56 %.

Antihyperglycaemic and tissue-repair effects of Myrmeleon formicarius extract in streptozotocin-induced diabetic mice

“…Results of this study showed that M. formicarius (antlion) extract exhibited glucose-lowering effects in streptozotocin-induced diabetic mice. This effect could be attributed to the presence of sulphonylurea- and metformin-like compounds in M. formicarius (antlion) larvae 21, 22, 28, 29. The presence of sulphonylurea compound was verified by the observation of a yellow band on the TLC plate, which is in line with the findings of a previous study 26 .…”

The Effect of Antlion (Myrmeleon sp.) Extract Towards Histopathology Image of Pancreas in Diabetic Mice

“…Antlion (Myrmeleon sp.) contains chemical compound such as metformin, ALA, and LA that can ameliorate cellular damages caused by either inflammatory process or ROS through various mechanisms (Canetti et al, 2014;Jiang et al, 2017;Muadifah et al, 2017;Malcicka, Visser and Ellers, 2018). Through its anti-inflammatory actions, Metformin was able to protect pancreatic islet damage caused by lowdose multiple injection of Streptozotocine in mice (Jiang et al, 2017).…”

A Validated Ultra Performance Liquid Chromatography Method for Quantification of Metformin in Human Plasma

“…Precipitation proteins are widely used for MTF analysis, although simple and fast but not specific and less effective in removing other analytical disorders. Pairing ion liquidliquid extraction [7][8][9][10] and solid liquid extraction [11] have also been developed to overcome the polarity of MTF. Ultrafiltration with the cation exchange column has also been reported but its sensitivity is low [12].…”