Lignin Content, Ligninase Enzyme Activity and in vitro Digestability of Sugarcane Shoots using Pleurotus ostreatus and Aspergillus oryzae at Different Fermentation Times

Abstract

This study aims to obtain the best lignin content, ligninase enzyme activity and in vitro digestibility value of fermented sugarcane shoots using Pleurotus ostreatus and Aspergillus oryzae at different fermentation times. The research process is divided into 2 stages. Stage 1 treatment samples include A1B1 = Sugarcane shoots fermented with Pleurotus ostreatus for 14 days; A1B2 = Sugarcane shoots fermented with Pleurotus ostreatus for 21 days; A1B3 = Sugarcane shoots fermented with Pleurotus ostreatus for 28 days; A2B1 = Sugarcane shoots fermented with Aspergillus oryzae for 14 days; A2B2 = Sugarcane shoots fermented with Aspergillus oryzae for 21 days; A2B3 = Sugarcane shoots fermented with Aspergillus oryzae for 28 days. The design used is a completely randomized design with a factorial pattern. Stage 2 treatment samples cover A = Sugarcane shoots fermented with Pleorotus ostreatus for 21 days; B = Sugarcane shoots fermented with Pleorotus ostreatus for 28 days; C = Sugarcane shoots fermented with Aspergillus oryzae for 21 days; D = Fermented sugarcane shoots with Aspergillus oryzae for 28 days. The design used was a randomized block design. The results showed that there was no interaction between the type of mold and the fermentation time on the lignin content (P>0.05), but there was an interaction with the enzyme activity of Laccase, LiP and MnP (P<0.05). Also, there were significant differences in the digestibility of protein, cellulose, hemicellulose, VFA and NH3 (P<005), however the digestibility of DM, OM, ADF, NDF and rumen fluid pH had no significant difference (P>0.05). It was concluded that sugarcane shoots fermented with Pleurotus ostreatus mold for 28 days got the best results with the value as follow, lignin content (11.55%), CP digestibility (57.90%), cellulose digestibility (50.25%), Hemicellulose digestibility (62.65%), Laccase enzyme activity (2.68 U/mL), LiP enzyme activity (19.44 U/mL), VFA (111.67 mM) and NH3 (10.48 mg/100 mL).

In Vitro Evaluation of Feed Quality of Fermented Tithonia diversifolia with Lactobacillus bulgaricus and Persea americana miller Leaves as Forages for Goat

“…Besides that, high nutrient content in fermented T. diversifolia contributed to the high digestibility in line with Pazla et al (2022). According to Pazla et al (2021d), lignin negatively correlates with ADF digestibility. High lignin content will decrease nutrient digestibility because rumen microbes cannot degrade lignin.…”

“…The total VFA in FTD5 increased due to its low crude fiber and lignin content. The duration of fermentation significantly affects the lignin content (Pazla et al, 2021d), so microbial rumen was not inhibited from doing degrading activity.…”

Nutrition Evaluation of Etawa Crossbreed Dairy Goat’s Milk as Human Food to Increase Immunity During The Covid-19 Pandemic

“…Forage eaten by livestock undergoes a fermentative process in the rumen by rumen microbes [9], [10]. The main product of fermentation in the rumen is VFA (Volatile fatty acids) [11], [12]. VFA consists of acetate, propionate and butyrate [13].…”

Production Performance, Feed Intake and Nutrient Digestibility of Etawa Crossbreed Dairy Goats Fed Tithonia (Tithonia diversifolia), Cassava Leaves and Palm Kernel Cake Concentrate

“…In contrast, the lignin content of PKCC is not much different from that of the firm concentrate. High lignin content in livestock rations reduces digestibility because lignin is a timber substance that cannot be digested using rumen microbes (Pazla et al 2020;Pazla et al 2021d;Ciptaan et al 2022).…”