Light induced DEP for immobilizing and orienting Escherichia coli bacteria

Miccio, Lisa; Marchesano, Valentina; Mugnano, Martina; Grilli, Simonetta; Ferraro, Pietro

doi:10.1016/j.optlaseng.2015.03.025

Cited by 42 publications

(27 citation statements)

References 41 publications

(53 reference statements)

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“…23 Furthermore, photorefractive properties of iron-doped LiNbO 3 can be used for electrode-free immobilization of bacteria via light-induced dielectrophoresis. 24 Finally, cell sorting can be achieved via acoustic waves by bonding LiNbO 3 to microfluidic devices. 25 Here, we investigate the potential of LiNbO 3 as a neuron substrate, providing charge and topography patterns to fluidically isolated axons, where the combined effects of chemotactic, charge, and topography cues can be studied in controlled microfluidic environments.…”

mentioning

confidence: 99%

Charge and topography patterned lithium niobate provides physical cues to fluidically isolated cortical axons

Kilinc

Blasiak

Baghban

et al. 2017

Applied Physics Letters

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In vitro devices that combine chemotactic and physical cues are needed for understanding how cells integrate different stimuli. We explored the suitability of lithium niobate (LiNbO3), a transparent ferroelectric material that can be patterned with electrical charge domains and micro/nanotopography, as a neural substrate. On flat LiNbO3 z-surfaces with periodically alternating charge domains, cortical axons are partially aligned with domain boundaries. On submicron-deep etched trenches, neurites are aligned with the edges of the topographical features. Finally, we bonded a bicompartmental microfluidic chip to LiNbO3 surfaces patterned by etching, to create isolated axon microenvironments with predefined topographical cues. LiNbO3 is shown to be an emerging neuron culture substrate with tunable electrical and topographical properties that can be integrated with microfluidic devices, suitable for studying axon growth and guidance mechanisms under combined topographical/chemical stimuli.

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confidence: 99%

Charge and topography patterned lithium niobate provides physical cues to fluidically isolated cortical axons

Kilinc

Blasiak

Baghban

et al. 2017

Applied Physics Letters

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“…However, recently, new results have been reported on the effect of the electrical torques acting on elongated particles and the possibility of inducing, not only their trapping, but also their alignment along the PV fields [21,31]. For anisotropic particles the tensor αij has, in principle, three different principal values along the three main perpendicular axes.…”

Section: Physical Basis Of the Pv Methodsmentioning

confidence: 99%

“…In 1D patterns, record-breaking periods of 2 µm were obtained. In parallel, Miccio et al [21] reported the trapping and orienting of live Escherichia coli bacteria over x-cut LiNbO3:Fe taking advantage of the PVE too. Different grating periods were optically recorded in the LiNbO3:Fe (12.5-100 µm).…”

Section: Biological Pvt Applicationsmentioning

confidence: 99%

“…Special mention also deserves the applications of PVT to biomedical problems (Section 5). Although preliminary and exploratory experiments [20][21][22] have been carried out so far, it is expected that applications in the biological field should experience a strong impulse in the near future. Therefore, we have devoted special attention in this review to summarily quote recent work on this exciting topic which has not been adequately reviewed so far.…”

Section: Introductionmentioning

confidence: 99%

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Recent Achievements on Photovoltaic Optoelectronic Tweezers Based on Lithium Niobate

et al. 2018

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Abstract:This review presents an up-dated summary of the fundamentals and applications of optoelectronic photovoltaic tweezers for trapping and manipulation of nano-objects on the surface of lithium niobate crystals. It extends the contents of previous reviews to cover new topics and developments which have emerged in recent years and are marking the trends for future research. Regarding the theoretical description of photovoltaic tweezers, detailed simulations of the electrophoretic and dielectrophoretic forces acting on different crystal configurations are discussed in relation to the structure of the obtained trapping patterns. As for the experimental work, we will pay attention to the manipulation and patterning of micro-and nanoparticles that has experimented an outstanding progress and relevant applications have been reported. An additional focus is now laid on recent work about micro-droplets, which is a central topic in microfluidics and optofluidics. New developments in biology and biomedicine also constitute a relevant part of the review. Finally, some topics partially related with photovoltaic tweezers and a discussion on future prospects and challenges are included.

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“…For example, Kano et al used a microfluidic device to immobilize microbes by DEP and found that the positive DEP was capable of selectively collecting live bacteria inside micropores. In addition, Miccio et al [32] adopted electrode-free DEP for immobilization and orientation of bacteria. This electrode-free DEP was light induced, achieved through ferroelectric iron-doped lithium niobate crystals used as substrates.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of nanocomposites and hybrid materials using microbial biotemplates

Shi

Ullah

et al. 2017

Adv Compos Hybrid Mater

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Microbes are important part of life that vary in sizes and shapes, diverse surface chemistry and biology, and porous nature of their cell walls. Besides their importance in industrial processes such as fermentation, these serve as biotemplates and provide a biomimetic approach for fabrication of multifarious complex constructs with predefined features, ordered composites and hybrid nanomaterials, microdevices, and micro/nanorobots through various strategies. The template or building blocks for such approaches can be bacterial, algal, and fungal cells or virus particles. Here, we have summarized recent advancements in biofabrication based on live microbes. Using engineering approaches and suitable methods, live microbes can be manipulated as functional Bmicro/nanodevices and -robots^to further perform biological functions such as replication, distribution, motility, formation of colonies, and secretion of metabolites at will. Biofabrication based on microbes provides effective methods to control and manipulate microbes as functional live building blocks to create micro/nanodevices and -robots for biomedical and energy applications.

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Light induced DEP for immobilizing and orienting Escherichia coli bacteria

Cited by 42 publications

References 41 publications

Charge and topography patterned lithium niobate provides physical cues to fluidically isolated cortical axons

Charge and topography patterned lithium niobate provides physical cues to fluidically isolated cortical axons

Recent Achievements on Photovoltaic Optoelectronic Tweezers Based on Lithium Niobate

Fabrication of nanocomposites and hybrid materials using microbial biotemplates

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