Light-Induced Decomposition of Indocyanine Green

Engel, Eva; Schraml, Rüdiger; Maisch, Tim; Kobuch, Karin; König, Burkhard; Szeimies, Rolf‐Markus; Hillenkamp, Jost; Bäumler, Wolfgang; Vasold, Rudolf

doi:10.1167/iovs.07-0911

Cited by 233 publications

(240 citation statements)

References 41 publications

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“…27 It is widely accepted that the potential toxic effects of ICG 10,11,31,32 as described clinically are very likely related to photosensitizing properties of ICG as described above and the decomposition products of the ICG molecule after illumination. 7,8,33 Importantly, other additional relevant tissue-dye interactions seem to have a role as a causative effect for the increase in tissue stiffness, as increased rigidity was also seen for BBG in our experiments. In contrast to ICG, BBG, a triarylmethane dye, has no known photosensitizing properties.…”

Section: Basement Membrane Rigidity C Haritoglou Et Alsupporting

confidence: 63%

Vital dyes increase the rigidity of the internal limiting membrane

Haritoglou

Mauell

Benoit

et al. 2013

Eye

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Purpose To assess the stiffness of the natural human internal limiting membrane (ILM) and evaluate potential changes of the mechanical properties following staining with brilliant blue (BB) and indocyanine green (ICG). Methods Unstained ILM specimens were obtained during ophthalmic surgical procedures. After removal, the specimens were dissected into five parts. Two fragments were stained with BB and ICG, respectively, for 1 min, another two specimens were stained similarly followed by additional subsequent illumination using a standard light source (PENTA LUX x 50, Ophthalmologische Systeme GmbH Fritz Ruck). The fifth part served as an untreated control. All specimens were then analyzed using atomic force microscopy (AFM) in contact mode with a scan rate of 0.6 Hz. Two scan regions of 10 Â 10 mm were chosen and stiffness was determined by using AFM in a force spectroscopy mode. The force curves were plotted with a data rate of 5000 Hz. In all specimens both the retinal side and vitreal side were analyzed.

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Section: Basement Membrane Rigidity C Haritoglou Et Alsupporting

confidence: 63%

Vital dyes increase the rigidity of the internal limiting membrane

Haritoglou

Mauell

Benoit

et al. 2013

Eye

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“…However, with exogenous chromophores, e.g., the indocyanine green (ICG), the efficiency of light interaction with tissue may be essentially higher and more selective [209][210][211][212][213]. The intensity and position of ICG absorption peaks depend upon the solvents used [214][215][216]. The solutions with complex compositions, e.g., such as water-alcohol-glycerol, stabilise the ICG absorption peaks [215].…”

Section: Photochemical and Photothermal Clearingmentioning

confidence: 99%

“…The next step of the interaction between the dye and the cell is related to the light action. The light causes photochemical reactions; for ICG they simultaneously follow two scenarios: 1) photodynamic reactions; 2) reactions yielding toxic products [216]. Depending on the intensity, the biological response of the cell may lead to reversible of irreversible injury of the cell membrane.…”

Section: Photochemical and Photothermal Clearingmentioning

confidence: 99%

Optical clearing of biological tissues: prospects of application in medical diagnostics and phototherapy

Genina

Bashkatov

Sinichkin

et al. 2015

JBPE

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Abstract.A review of specific features and methods of optical clearing and related interaction of light with tissues is presented. Physical and molecular mechanisms of immersion, compression, and photodynamic/photothermal optical clearing of some fibrous and cellular tissues are discussed. The possibility of efficient control of the tissue optical properties, particularly, the reduction of light scattering in tissues is demonstrated, which facilitates the increased efficiency of various optical visualisation methods (optical biopsy) used in medical purposes. © 2015 Samara State Aerospace University (SSAU).Keywords: tissue, optical clearing, optical diagnostics, imaging. 1, 404-413 (1997). 5. C. L. Smithpeter, A. K. Dunn, A. J. Welch, and R. Richards-Kortum, "Penetration depth limits of in vivo confocal reflectance imaging," Appl. Opt. 37, 2749Opt. 37, -2754Opt. 37, (1998. 6. V. V. Tuchin, L. V. Wang, and D. A. Zimnyakov, Optical Polarization in Biomedical Applications, SpringerVerlag, New York, NY, USA (2006). 7. R. Drezek, A. Dunn, and R. Richards-Kortum, "Light scattering from cells: finite-difference time-domain simulations and goniometric measurements," Appl. Opt. 38(16), 3651-3661 (1999). 8. K. Sokolov, R. Drezek, K. Gossagee, and R. Richards-Kortum, "Reflectance spectroscopy with polarized light:is it sensitive to cellular and nuclear morphology," Opt. Express 5, 302-317 (1999). 9. D. W. Leonard, and K. M. Meek, "Refractive indices of the collagen fibrils and extrafibrillar material of the corneal stroma," Biophysical J. 72, 1382-1387 (1997). 10. A. G. Borovoi, E. I. Naats, and U. G. Oppel, "Scattering of light by a red blood cell," J. Biomed. Opt. 3, 364-372 (1998). 11. A. N. Yaroslavsky, A. V. Priezzhev, J. Rodriguez, I. V. Yaroslavsky, and H. Battarbee, "Optics of blood,"Chap. 2 in Handbook of Optical Biomedical Diagnostics, V. V. Tuchin, Ed., pp. 169-216, PM107 SPIE Press, Bellingham, WA, USA (2002). 12. G. Mazarevica, T. Freivalds, and A. Jurka, "Properties of erythrocyte light refraction in diabetic patients," J.Biomed. Opt. 7, 244-247 (2002). 13. M. Friebel, and M. Meinke, "Model function to calculate the refractive index of native hemoglobin in the wavelength range of 250-1100 nm dependent on concentration," Appl. Opt. 45(12), 2838-2842 (2006). Appl. Opt. 35(19), 3413-3420 (1996). 34. D. Zhu, J. Wang, Z. Zhi, X. Wen, and Q. Luo, "Imaging dermal blood flow through the intact rat skin with an optical clearing method," J. Biomed. Opt. 15, 026008 (2010 241. K. König, G. Flemming, and R. Hibst, "Laser-induced autofluorescence spectroscopy of dental caries lesion," Cell. Mol. Biol. 44, 1293-1300. 242. E. G. Borisova, T. T. Uzunov, and L. A. Avramov, "Early differentiation between caries and tooth demineralization using laser-induced autofluorescence spectroscopy," Lasers Surg. Med. 34, 249-253 (2004 -20(12), 1512-1516 (1984). 245. K. Konig, H. Schneckenburger, and R. Hibst, "Time-gated in vivo autofluorescence imaging of dental caries,"Cell. Mol. Biol. 45, 233-239 (1999). 246. E. Borisova, P. Tr...

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“…Direct toxicity, upregulation of apoptosis-related genes, 19 hypoosmolarity of the solvent, 20 intraoperative retinal illumination, 21,22 and the role of sodium were hypothesized as possible factors that could increase retinal ICG toxicity. 23,24 There are several limitations in our study.…”

Section: Discussionmentioning

confidence: 99%

The structural effect of intravitreal Brilliant blue G and Indocyanine green in rats eyes

Ooi

Khang

Naidu

et al. 2012

Eye

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Purpose To compare the potential retinal toxicity of two commercially Brilliant blue G dyes (Brilliant Peel and Ocublue Plus) and Indocyanine green (ICG) at usual clinical concentration. Methods Brilliant Peel 0.025% (n ¼ 9), Ocublue Plus 0.025% (n ¼ 9), and ICG 0.05% (n ¼ 9) were injected intravitreally into Sprague-Dawley rat left eyes with balanced salt solution injected in the contralateral eyes as control. Evaluation of the effect of the dyes on retinal architecture was done by histological analysis of neurosensory retinal thickness and retinal ganglion cell (RGC) counts 7 days after intravitreal injection. Paired t-test was done to detect the presence of biologically significant thinning in neurosensory retina and five retinal layers for each dye (paired t-tests). One-way ANOVA and Tukey's Honestly Significant Difference test were used to assess whether different dyes caused significant thinning in mean neurosensory retinal thickness and reduction of mean RGC density. Results Eyes treated with ICG had significantly thinner mean total neurosensory retinal thickness compared with the control eyes (P-value ¼ 0.01), followed by those treated with Ocublue Plus (P-value ¼ 0.03). Brilliant Peel did not cause significant thinning in any of the five retinal layers (all P-values40.05). No significant difference in mean thinning of the total retinal thickness was detected between dyes (P-value ¼ 0.11). The mean thickness of the photoreceptor outer segment and outer plexiform layers were significantly reduced in ICG-injected eyes when compared with the control eyes (P-value ¼ 0.02). No significant difference in mean thinning between the three dyes was detected at all five retinal layers using one-way ANOVA (all P-values40.35). RGC density was significantly reduced for ICG (P-value ¼ 0.01) but only marginally for Ocublue Plus (P-value ¼ 0.05). No significant reduction in RGC density was observed for Brilliant Peel (P-value ¼ 0.2). Conclusion Intravitreal Brilliant Peel is safe to rats retina. The retinal thinning and reduction in RGC density induced by Ocublue Plus requires further studies to determine the safety profile of this product. Potential retinal toxicity is seen with ICG 0.05%.

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Light-Induced Decomposition of Indocyanine Green

Abstract: ICG is decomposed by light within a self-sensitized photo oxidation. The decomposition products reduce the viability of RPE cells in vitro. The toxic effects of decomposed ICG should be further investigated under in vivo conditions.

Cited by 233 publications

References 41 publications

Vital dyes increase the rigidity of the internal limiting membrane

Vital dyes increase the rigidity of the internal limiting membrane

Optical clearing of biological tissues: prospects of application in medical diagnostics and phototherapy

The structural effect of intravitreal Brilliant blue G and Indocyanine green in rats eyes

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