The structural effect of intravitreal Brilliant blue G and Indocyanine green in rats eyes

Abstract

Purpose To compare the potential retinal toxicity of two commercially Brilliant blue G dyes (Brilliant Peel and Ocublue Plus) and Indocyanine green (ICG) at usual clinical concentration. Methods Brilliant Peel 0.025% (n ¼ 9), Ocublue Plus 0.025% (n ¼ 9), and ICG 0.05% (n ¼ 9) were injected intravitreally into Sprague-Dawley rat left eyes with balanced salt solution injected in the contralateral eyes as control. Evaluation of the effect of the dyes on retinal architecture was done by histological analysis of neurosensory retinal thickness and retinal ganglion cell (RGC) counts 7 days after intravitreal injection. Paired t-test was done to detect the presence of biologically significant thinning in neurosensory retina and five retinal layers for each dye (paired t-tests). One-way ANOVA and Tukey's Honestly Significant Difference test were used to assess whether different dyes caused significant thinning in mean neurosensory retinal thickness and reduction of mean RGC density. Results Eyes treated with ICG had significantly thinner mean total neurosensory retinal thickness compared with the control eyes (P-value ¼ 0.01), followed by those treated with Ocublue Plus (P-value ¼ 0.03). Brilliant Peel did not cause significant thinning in any of the five retinal layers (all P-values40.05). No significant difference in mean thinning of the total retinal thickness was detected between dyes (P-value ¼ 0.11). The mean thickness of the photoreceptor outer segment and outer plexiform layers were significantly reduced in ICG-injected eyes when compared with the control eyes (P-value ¼ 0.02). No significant difference in mean thinning between the three dyes was detected at all five retinal layers using one-way ANOVA (all P-values40.35). RGC density was significantly reduced for ICG (P-value ¼ 0.01) but only marginally for Ocublue Plus (P-value ¼ 0.05). No significant reduction in RGC density was observed for Brilliant Peel (P-value ¼ 0.2). Conclusion Intravitreal Brilliant Peel is safe to rats retina. The retinal thinning and reduction in RGC density induced by Ocublue Plus requires further studies to determine the safety profile of this product. Potential retinal toxicity is seen with ICG 0.05%.

Natural Course and Surgical Management of High Myopic Foveoschisis

“…Dyes should be used for ILM removal in highly myopic eyes because the ILM is transparent and fragile in these cases. In recent studies, the dyes used for the peeling of ILM in foveoschisis were triamcinolone acetonide or indocyanine green [20,21,22], and not Brilliant Blue G. In fact, indocyanine green has recently been reported to have a potential retinal toxicity compared with Brilliant Peel in an experimental study [23]. In our opinion, Brilliant Blue G staining should be considered in all cases with myopic foveoschisis to make the surgery easy and effective, and can lead to anatomical success as we report.…”

Osmolarity and spectrophotometric property of brilliant blue green define the degree of toxicity on retinal pigment epithelial cells exposed to surgical endoilluminator

“…However, there are established adverse events, such as visual field changes and retinal pigment epithelial (RPE) toxicity, after ICG-assisted peeling of the ILM 3–5. Brilliant blue green (BBG), among several other dyes, has revealed a similar affinity to stain ILM with minimal toxicity 6,7…”

Safety of Brilliant Blue G

“…[1][2][3][4][5] However, there is very little clinical experience of Aflibercept in polypoidal choroidal vasculopathy. We present our favourable experience with Aflibercept in the treatment of persistent PED after photodynamic therapy (PDT) combined with Ranibizumab.…”