Ligand-exchange chromatography of nucleases

A Near-Infrared Luminescent Label Based on YbIII Ions and Its Application in a Fluoroimmunoassay

“…[15] Direct introduction of the methyleneiminodiacetic acid groups, as used in the production of Fx, [16] was not successful. Reaction of iminodiacetic acid dimethyl ester [19] with 2 [20] and subsequent hydrolysis of the ester yielded 3, the desired nitro precursor to FxITC. Reduction of 3 using H 2 /Raney Ni proceded cleanly, but gave rise to the dinickel complex of 4, which can be observed with electrospray mass spectrometry.…”

Synthesis of Complexes of Some Transition Metals with Nitrogen Donor Macrocyclic Ligands

“…Iminodiacetic acid was used as the starting material for the preparation of dimethyl iminodiacetate hydrochloride [18] and this hydrochloride was used to obtain dimethyl iminodiacetate. [19] Preparation of Iminodiacetic Acid Dihydrazide To a cooled solution (5°C) of 85% hydrazine monohydrate (16.20 mL, 16.72 g, 334 mmol) in methanol (3 mL), a solution of dimethyl iminodiacetate (21.50 g, 133.6 mmol) in dry methanol (50 mL) was added with stirring.…”

Chromatographic methods of mackerel proteases isolation

“…After that 46 ml of sodium methoxide in methanol (obtained by desolving 4.6 g of sodium in methanol) were added to suspension of 20 g of iminodiacetic acid dimethyl ester hydrochloride in 200 ml of anhydrous DMSO (obtained as described in [ 111). The obtained solution was filtered off and added to epoxyactivated agarose.…”