Abstract:A possibility is shown of producing acidic and alkaline proteases from water fraction obtained during comprehensive processing of hydrobionts. The proteases are obtained by the affinity and ligand-exchange chromatography methods, without resorting to a non-technological stage of enzyme precipitation by ammonia sulphate or organic solvents. The level of specific activity thus achieved for acidic mackerel proteases is 300 u.a./g, for alkaline mackerel proteases 630 u.a./g.
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