Lichen metabolites prevent UV light and nitric oxide-mediated plasmid DNA damage and induce apoptosis in human melanoma cells

Russo, Alessandra; Piovano, Marisa; Lombardo, Laura; Garbarino, Juan A.; Cardile, Venera

doi:10.1016/j.lfs.2008.07.012

Cited by 97 publications

(81 citation statements)

References 47 publications

(55 reference statements)

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“…When DNA is exposed to UV light, in the presence of H 2 O 2 , this situation is leading to produce free hydroxyl radicals, and change native formation of DNA (scDNA), produced ocDNA and linear DNA (linDNA). [22][23][24][25][26][27] The addition of the water and ethanol extracts of HP at 25µg/ml-200µg/ml concentration to the reaction mixture prevent to change the formation of linDNA, and help to protect the native formation of DNA ( Figure.1). Figure 1 shows the electrophoretic band pattern of pBR322 plasmid DNA after exposed to UV/H 2 O 2 in the absence and presence of the extracts of HP in a dose dependent manner (concentration of the extracts ranging from 25µg/ml to 200µg/ml): first four lines are control samples, UV irritation of DNA in the absence of …”

Section: Antioxidant Activities Of Hpmentioning

confidence: 99%

Variation in Total Polyphenolic Contents, DNA Protective Potential and Antioxidant Capacity from Aqueous and Ethanol Extracts in Different Plant Parts of Hypericum perforatum L.

Şekeroğlu¹,

Urlu²,

Kulak³

et al. 2017

IJPER

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Background and Purpose: Hypericum perforatum belonging to the family Hypericaceae is a reputed medicinal plant including a wide ranges of important phytochemical components. Chlorogenic acid, rutin, hyperoside, quercitrin, quercetin, pseudohypericin, hypericin and hyperforin are of the major components. Crude extract and individual compounds of H. perforatum have been reported to exert antidepressant, antibiotic, and antitumoral activities. It is worthy to note that the quantity and efficacies of the crude extracts or individual compound are not constant, which are strongly influenced by different climatic conditions, harvesting times, harvested plant organs and post-harvest practices. Hence, numerous studies on H. perforatum collected from different parts of the World are carried out for their desired quality and biological efficacy. Methods: Wild collected plant materials were dried and preserved with a voucher specimen number and were extracted using maceration at room temperature for 24 h in dark. Subsequently, extracts were screened for their phenolic and flavonoid contents, plausible antioxidant activities using two methods namely DPPH radical scavenging and ferric-reducing antioxidant power (FRAP) assays and DNA protective activities. Results: The highlights of the study were are listed as 1) the highest total phenolic content in ethanol extracts of leaf, ii) the highest total flavonoid content in flower, iii) DPPH scavenging activity in leaf (80.51 %), flower (63.42 %) and stem (48.20 %), iv) highest ferric reduction capacity in ethanol extracts of stem were determined. Also, potent DNA protection activity was observed even at the lowest concentration value (25µg/ml) of the extracts. Conclusion: The phenolic content and strong antioxidant activities of ethanol extracts of different parts of the plant are reported. All the extracts exhibited strong DNA protective activities in response to the UV radiation in the presence of hydrogen peroxide.

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Section: Antioxidant Activities Of Hpmentioning

confidence: 99%

Variation in Total Polyphenolic Contents, DNA Protective Potential and Antioxidant Capacity from Aqueous and Ethanol Extracts in Different Plant Parts of Hypericum perforatum L.

Şekeroğlu¹,

Urlu²,

Kulak³

et al. 2017

IJPER

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“…4 Many different secondary metabolites have been isolated from lichens including aliphatic, cycloaliphatic, aromatic and terpenic components that are unique with respect to those found in higher plants. 5 Screening of lichen extracts has revealed the frequent occurrence of metabolites with antibiotic, antimycobacterial, antiviral, antitumor, analgesic, antiallergic and antipiretic properties, and also substances that inhibit plant growth as well as several enzymes. 4,6 Xanthone compounds represent a class of these lichen substances, but they are widely distributed in nature, 2 and can also be isolated from higher plants and microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Chemical modifications of a natural xanthone and antimicrobial activity against multidrug resistant Staphylococcus aureus and cytotoxicity against human tumor cell lines

Micheletti¹,

Honda²,

Lima³

et al. 2011

Quím. Nova

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A series of 15 ω-aminoalkoxylxanthones containing methyl, ethyl, propyl, tert-butylamino and piperidinyl moieties were synthesized from a natural xanthone isolated from a lichen species. These compounds were tested for their in vitro antibacterial properties against Gram-positive and Gram-negative bacteria and cytotoxicity against a number of human tumor cell lines was too evaluated. The newly synthesized derivatives revealed selective activity against Staphylococcus aureus (Gram-positive), and the most promising results are for a multidrug resistant strain, for which six of these compounds showed good activity (MICs 4 µg/mL). Many derivatives inhibited tumor cells growth and most compounds were active on multiple lines.

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“…Human skin irradiation with both UV-A and UV-B increases the production of reactive oxygen species (ROS) and reactive nitrogen species (RNS) and this contributes to inflammation, immunosuppression, gene mutation and carcinogenesis. The depsidone pannarin also acts as a potent dose-dependent agent for superoxide scavenging (Russo et al, 2008). Irradiation of pannarin, 1′-chloropannarin and atranorin with 366 nm light leads to significant hemolysis in a red cell suspension.…”

Section: Interactions With Photodynamic Astringentsmentioning

confidence: 99%

“…By using a human melanoma cells (M14 cell line), Russo et al (2008) found that sphaerophorin (depside) and pannarin (depsidone) showed a protective effect on plasmid DNA and exhibited a superoxide dismutase like effect. The data obtained from cell culture show that these lichen metabolites inhibit the growth of melanoma cells, inducing their apoptotic cell death, demonstrated by the fragmentation of genomic DNA and by a significant increase of caspase-3 activity, and correlated, at least in part, to the increase of ROS generation.…”

Section: Cytotoxicitymentioning

confidence: 99%

Bioproduction of Depsidones for Pharmaceutical Purposes

Legaz¹,

Armas²,

Vicente³

2011

Drug Development - A Case Study Based Insight Into Modern Strategies

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Lichen metabolites prevent UV light and nitric oxide-mediated plasmid DNA damage and induce apoptosis in human melanoma cells

Cited by 97 publications

References 47 publications

Variation in Total Polyphenolic Contents, DNA Protective Potential and Antioxidant Capacity from Aqueous and Ethanol Extracts in Different Plant Parts of Hypericum perforatum L.

Variation in Total Polyphenolic Contents, DNA Protective Potential and Antioxidant Capacity from Aqueous and Ethanol Extracts in Different Plant Parts of Hypericum perforatum L.

Chemical modifications of a natural xanthone and antimicrobial activity against multidrug resistant Staphylococcus aureus and cytotoxicity against human tumor cell lines

Bioproduction of Depsidones for Pharmaceutical Purposes

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