Variation in Total Polyphenolic Contents, DNA Protective Potential and Antioxidant Capacity from Aqueous and Ethanol Extracts in Different Plant Parts of Hypericum perforatum L.

Abstract

Background and Purpose: Hypericum perforatum belonging to the family Hypericaceae is a reputed medicinal plant including a wide ranges of important phytochemical components. Chlorogenic acid, rutin, hyperoside, quercitrin, quercetin, pseudohypericin, hypericin and hyperforin are of the major components. Crude extract and individual compounds of H. perforatum have been reported to exert antidepressant, antibiotic, and antitumoral activities. It is worthy to note that the quantity and efficacies of the crude extracts or individual compound are not constant, which are strongly influenced by different climatic conditions, harvesting times, harvested plant organs and post-harvest practices. Hence, numerous studies on H. perforatum collected from different parts of the World are carried out for their desired quality and biological efficacy. Methods: Wild collected plant materials were dried and preserved with a voucher specimen number and were extracted using maceration at room temperature for 24 h in dark. Subsequently, extracts were screened for their phenolic and flavonoid contents, plausible antioxidant activities using two methods namely DPPH radical scavenging and ferric-reducing antioxidant power (FRAP) assays and DNA protective activities. Results: The highlights of the study were are listed as 1) the highest total phenolic content in ethanol extracts of leaf, ii) the highest total flavonoid content in flower, iii) DPPH scavenging activity in leaf (80.51 %), flower (63.42 %) and stem (48.20 %), iv) highest ferric reduction capacity in ethanol extracts of stem were determined. Also, potent DNA protection activity was observed even at the lowest concentration value (25µg/ml) of the extracts. Conclusion: The phenolic content and strong antioxidant activities of ethanol extracts of different parts of the plant are reported. All the extracts exhibited strong DNA protective activities in response to the UV radiation in the presence of hydrogen peroxide.

Cancer preventive and neuroprotective potentials of red hulls, kernels and oleo-gum resins from Pistachio

“…Free Radical Scavenging Activity Antioxidant activities of the extracts were determined using in vitro 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) methods (Gezici et al, 2017;Sekeroglu et al, 2017;Gezici and Sekeroglu, 2019b). Ascorbic acid was used a commercial standard for DPPH assay, whilst Trolox was used a commercial standard for ABTS assay.…”

A study on Turmeric (Curcuma longa L.): Multifunctional agents for the management of oxidative damage, neurodegeneration and cancer

“…In vitro antioxidant methods including 2,2-diphenyl-1picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6sulphonic acid) (ABTS), ferric reducing antioxidant power (FRAP), and cupric ion reducing capacity (CUPRAC) were performed to reveal antioxidant activity of the extracts. 13,18,21,22 The extracts and commercial antioxidant standards were dissolved in DMSO at different concentrations, ranging from 100 μg mL −1 to 1000 μg mL −1 for the antioxidant assays.…”

“…capacities by our research group. [12][13][14][15][16][17][18][19][20][21][22][23][24][25] The main purpose of these researches are to contribute to discover new and effective natural products for prevention and treatment of oxidative stress-related diseases and disorders. Take into consideration our previous researches on MAPs and plant-derived natural products, total polyphenolic contents, in vitro antioxidant effects, neuroprotective and anticancer activities of the extracts obtained from the rhizomes of C. longa L. were aimed to evaluate in the presented research.…”

A Histopathological, Immunohistochemical and Biochemical Investigation on the in vitro Antioxidant, Myeloprotective, Hematoprotective and Hepatoprotective Effects of Hypericum triquetrifolium Seed Extract Against Cyclophosphamide-Induced Toxicity

“…CP is used in combination with different detoxifying and protective agents with the aim of decreasing or removing its adverse toxic effects [14]. Extracts of herbal medicines are shown to have beneficial therapeutic effects such as antioxidant, anticancer, anti-inflammatory, antimicrobial effects [15,16]. The antioxidants can minimize the toxic side effects of chemotherapeutic agents and allow the use of higher doses of anticancer drugs [5,17].…”