LED light for in vitro and ex vitro efficient growth of economically important highbush blueberry (Vaccinium corymbosum L.)

Hung, Cao Dinh; Hong, Chang–Hee; Kim, Seon-Ki; Lee, Kyuhan; Park, Jea-Young; Nam, Min-Woo; Choi, Dong Geun; Lee, Hye-In

doi:10.1007/s11738-016-2164-0

Cited by 86 publications

(51 citation statements)

References 43 publications

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“…The results of the present study indicated that the use of blue light in in vitro conservation in minimum growth was promising and cast a new perspective on this area. Inhibition of plant development related to the use of blue light during the in vitro process, has been reported in crops such as potato (Luz et al, 2015), chrysanthemum (Kurilčik et al, 2008), blueberry (Hung et al, 2016) and grape (Poudel et al, 2008). In general, the blue component inhibited elongation of the plantlets and root formation in vitro.…”

Section: Resultsmentioning

confidence: 99%

“…Light sources with different wavelengths from light emitting diodes (LED) have been widely used in in vitro propagation in different crops such as sugarcane, anthurium and blueberry, with the purpose of increasing production and improving the quality of the plants (Maluta et al, 2013;Martinez-Estrada et al, 2016;Hung et al, 2016). Such light sources may have an effect on in vitro development with significant changes in the arrangements of the thylakoids in the chloroplasts, which reduces the size of the plants, in emission and length of the roots, number of stomata in the leaves and chlorophyl content (Maluta et al, 2013;Vieira et al, 2015;Macedo et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Slow-grown in vitro conservation of Heliconia champneiana cv. Splash under different light spectra

Rodrigues

Arruda

Forti

2018

Sci. agric. (Piracicaba, Braz.)

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, maintained in vitro for two time periods, namely P1, 6 weeks and P2, twelve weeks, and were statistically evaluated after each interval with respect to: height, fresh weight, number and length of roots, number of leaves, and pseudostem diameter, in addition to the rate of acclimatization in percentage terms. Treatment B100 presented the lowest level of development in the two periods, and 100 % survival in acclimatization.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Slow-grown in vitro conservation of Heliconia champneiana cv. Splash under different light spectra

Rodrigues

Arruda

Forti

2018

Sci. agric. (Piracicaba, Braz.)

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“…However, 2iP showed longer shoots with a higher number of nodes [23]. For initial culture of highbush blueberry, 1 mg L −1 (2.85 µM) zeatin riboside was used [24]. In lowbush blueberry, the authors tested 0, 2.3, 4.6, or 9.10 µM ZEA on the elongation of shoots, and concentrations of 2.3 and 4.6 µM gave the best response [25].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Establishment of ‘Delite’ Rabbiteye Blueberry Microshoots

Schuchovski

Biasi

2019

Horticulturae

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Micropropagation is an important technique for clonal mass propagation and a tool for in vitro studies. One of the first steps to overcome in this process is the establishment of new explants in vitro. ‘Delite’ rabbiteye blueberry was cultured in vitro with four cytokinins (zeatin (ZEA), 6-(γ-γ-dimethylallylamino)-purine (2iP), 6-benzylaminopurine (BAP), and kinetin (KIN)) at eight concentrations (0, 2.5, 5, 10, 20, 30, 40, and 50 µM). Additionally, nine combinations of nitrogen salts were tested, using Woody Plant Medium (WPM) and a modified WPM as the basic medium. ZEA and 2iP showed better responses, but ZEA was superior at lower (2.5 µM) concentrations (89.7% survival, 81.3% shoot formation, 1.3 shoots, 13.8 mm shoot length, 10.0 leaves). BAP and KIN showed very low responses. In the combinations of salts with modified WPM, no differences were observed. However, the original WPM with treatments of 0.5 × NH4NO3 and 1 × Ca(NO3)2, 0.5 × NH4NO3 and 0.5 × Ca(NO3)2, and the modified WPM alone showed the lowest rates of survival and shoot formation and the shortest shoot lengths. The highest shoot lengths were observed in treatments with the original WPM, 1.5 × NH4NO3 and 0.5 × Ca(NO3)2, and 1.5 × NH4NO3 and 1.5 × Ca(NO3)2. This initial study with ‘Delite’ can be the basis for further experiments with different combinations of salts, 2iP, and ZEA.

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“…For example, beech (Fagus sylvatica L.), holm oak (Quercus ilex L.), and wild cherry (Prunus avium) seedlings grown under a wide continuous spectrum LED showed significantly longer shoots than plants grown under FL and also beech had 40% and 110% greater shoot fresh and dry matter, respectively (Astolfi et al, 2012). Also, Zantedeschia albomaculata (calla lily) and Vaccinium corymbosum (highbush blueberry) shoot elongation as well as fresh and dry weights were significantly increased when cultures were kept under red LED in comparison to FL (Chang et al, 2003;Hung et al, 2016). Light quality influences plant development and physiology because it affects (FL); direita: brotos sob diodos emissores de luz de alta potência (HP-LED); barra: 1cm.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Development of Yellow Lapacho (Bignoniaceae) Using High-Power Light Emitting Diode

2018

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Handroanthus ochraceus (yellow lapacho) is a medicinal, ornamental and timber tree which can be propagated by in vitro culture. Conventional methods use fluorescent lighting (FL), whereas light emitting diode (LED) has been used for this purpose only recently. The aim of this work was to evaluate the effects of FL and high-power LED (HP-LED) on the in vitro multiplication and rooting of yellow lapacho at different irradiances (15 to 60 µmol m-2s-1). Epicotyls obtained from half-siblings was multiplicated in WPM (Woody Plant Medium) supplemented with 20 µM benzilaminopurine and 1 mM IBA (indolebutiric acid). For rooting, shoots were cultured for 3 days in ½WPM supplemented with 50 µM IBA and for 42 days in auxin-free ½WPM under HP-LED or FL lighting. Under HP-LED, the multiplication rate of shoots increased significantly (61%) from 20 to 40 µmol m-2s-1 respect to FL. Differences in abaxial stomatal density and size were observed between light sources at 20 µmol m-2s-1. High HP-LED irradiance produced the highest rooting percentage. In the rooting stage, the marginal means of treatments without factors interaction showed that HP-LED irradiances significantly increased shoot length by 20%, shoot fresh weight by 77% and shoot dry weight by 30% in comparison to the values under FL. The maximum values calculated from the regression curves were around 50 µmol m-2 s-1 for HP-LED for all parameters except root lenght whereas were around 20 µmol m-2 s-1 for FL for all parameters except fresh and dry weigth of shoot. Here we show that HP-LED lighting improve in vitro culture of H. ochraceus, reduced 81% energy consumption respect to FL and uses only a multispectral LED instead of different single color LEDs. Therefore, HP-LED could be useful for the micropropagation of tree species contributing to sustainable agriculture and ecological restoration of degraded areas.

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LED light for in vitro and ex vitro efficient growth of economically important highbush blueberry (Vaccinium corymbosum L.)

Cited by 86 publications

References 43 publications

Slow-grown in vitro conservation of Heliconia champneiana cv. Splash under different light spectra

Slow-grown in vitro conservation of Heliconia champneiana cv. Splash under different light spectra

In Vitro Establishment of ‘Delite’ Rabbiteye Blueberry Microshoots

In Vitro Development of Yellow Lapacho (Bignoniaceae) Using High-Power Light Emitting Diode

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