Leaf proteome analysis provides insights into the molecular mechanisms of bentazon detoxification in rice

Fang, Yiman; Lu, Haiping; Chen, Si; Zhu, Kun Yan; Song, Hao; Qian, Haifeng

doi:10.1016/j.pestbp.2015.06.003

Cited by 17 publications

(10 citation statements)

References 48 publications

(52 reference statements)

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“…Here, the average number of spots detected was 643 and, no statistically significant difference was found between or within treatments (plant varieties and growing conditions) in either experiment (one-way ANOVA, P < 0.05; data not shown). Comparative proteomic studies using 2D-DIGE analysis in plant leaves have shown an average number of detected spots ranging from 500 to 900, which agrees with our results [ 41 – 43 ].…”

Section: Resultssupporting

confidence: 92%

Molecular responses of genetically modified maize to abiotic stresses as determined through proteomic and metabolomic analyses

et al. 2017

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Some genetically modified (GM) plants have transgenes that confer tolerance to abiotic stressors. Meanwhile, other transgenes may interact with abiotic stressors, causing pleiotropic effects that will affect the plant physiology. Thus, physiological alteration might have an impact on the product safety. However, routine risk assessment (RA) analyses do not evaluate the response of GM plants exposed to different environmental conditions. Therefore, we here present a proteome profile of herbicide-tolerant maize, including the levels of phytohormones and related compounds, compared to its near-isogenic non-GM variety under drought and herbicide stresses. Twenty differentially abundant proteins were detected between GM and non-GM hybrids under different water deficiency conditions and herbicide sprays. Pathway enrichment analysis showed that most of these proteins are assigned to energetic/carbohydrate metabolic processes. Among phytohormones and related compounds, different levels of ABA, CA, JA, MeJA and SA were detected in the maize varieties and stress conditions analysed. In pathway and proteome analyses, environment was found to be the major source of variation followed by the genetic transformation factor. Nonetheless, differences were detected in the levels of JA, MeJA and CA and in the abundance of 11 proteins when comparing the GM plant and its non-GM near-isogenic variety under the same environmental conditions. Thus, these findings do support molecular studies in GM plants Risk Assessment analyses.

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Section: Resultssupporting

confidence: 92%

Molecular responses of genetically modified maize to abiotic stresses as determined through proteomic and metabolomic analyses

et al. 2017

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“…In plants, the induction of oxidative stress can occur in different cellular compartments due to the activation of distinct signal transduction pathways that promote physiological responses and regulate normal cellular processes (Mittler, 2017; Shekhawat and Verma, 2010). Additionally, previous studies have reported differential gene expression as a mechanism of cellular defense in plants undergoing herbicide treatment (Fang et al, 2015; Liang et al, 2012).…”

mentioning

confidence: 96%

Gene Expression Related to Oxidative Stress Induced by Herbicides in Rice

Agostinetto¹,

Benemann²,

Cechin³

et al. 2019

Agronomy Journal

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Core Ideas UBQ5 was the most stable reference gene for gene expression analysis in rice treated with herbicides. The herbicides increased gene expression associated with defense against oxidative stress. OsHO‐1 showed the greatest change in expression in response to herbicide treatment. ABSTRACT Herbicides are stressors that can have negative effects on plants. In Oryza sativa (L.), differential gene expression may be evaluated through real‐time reverse transcription quantitative polymerase chain reaction (RT‐qPCR). The aim of this study was to evaluate the stability of candidate reference genes and to quantify the relative expression of oxidative stress genes at different times (12, 24, 48, and 96 hours after treatment [HAT]) with penoxsulam, cyhalofop‐butyl, and bentazon herbicides. NormFinder, BestKeeper, and GeNorm software and the comparative ∆Ct method were used to assess expression stability and to determine the RT‐qPCR threshold values of the candidate reference genes. The UBQ5 gene was the most stable among the reference genes analyzed. The gene expression results showed upregulation of OsCAT and OsMnSOD1 genes at all times after herbicide application. The OsAPX2 and OsGST3 genes showed increased gene expression at 12 and 96 HAT for all herbicides. The OsHO‐1 gene had the most significant expression changes, with maximum expression levels at 24 HAT with bentazon and at 96 HAT with penoxsulam and cyhalofop‐butyl. Overall, antioxidant system gene expression increased after the application of bentazon, penoxsulam, and cyhalofop‐butyl in rice.

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“…In most cases, GSH-related enzymes are induced or otherwise modified rapidly after the imposition of stress, illustrating the central role of redox maintenance. For example, 2D-DIGE analyses of O. sativa response to bentazon treatment [46] and Anabena spp. tolerance to butachlor [47] demonstrated the upregulation of several xenome enzymes including GSTs.…”

Section: Discussionmentioning

confidence: 99%

Proteomic and biochemical assays of glutathione-related proteins in susceptible and multiple herbicide resistant Avena fatua L.

Burns

Keith

Refai

et al. 2017

Pesticide Biochemistry and Physiology

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Extensive herbicide usage has led to the evolution of resistant weed populations that cause substantial crop yield losses and increase production costs. The multiple herbicide resistant (MHR) Avena fatua L. populations utilized in this study are resistant to members of all selective herbicide families, across five modes of action, available for A. fatua control in U.S. small grain production, and thus pose significant agronomic and economic threats. Resistance to ALS and ACCase inhibitors is not conferred by target site mutations, indicating that non-target site resistance mechanisms are involved. To investigate the potential involvement of glutathione-related enzymes in the MHR phenotype, we used a combination of proteomic, biochemical, and immunological approaches to compare their constitutive activities in herbicide susceptible (HS1 and HS2) and MHR (MHR3 and MHR4) A. fatua plants. Proteomic analysis identified three tau and one phi glutathione S-transferases (GSTs) present at higher levels in MHR compared to HS plants, while immunoassays revealed elevated levels of lambda, phi, and tau GSTs. GST specific activity towards 1-chloro-2,4-dinitrobenzene was 1.2-fold higher in MHR4 than in HS1 plants and 1.3- and 1.2-fold higher in MHR3 than in HS1 and HS2 plants, respectively. However, GST specific activities towards fenoxaprop-P-ethyl and imazamethabenz-methyl were not different between untreated MHR and HS plants. Dehydroascorbate reductase specific activity was 1.4-fold higher in MHR than HS plants. Pretreatment with the GST inhibitor NBD-Cl did not affect MHR sensitivity to fenoxaprop-P-ethyl application, while the herbicide safener and GST inducer mefenpyr reduced the efficacy of low doses of fenoxaprop-P-ethyl on MHR4 but not MHR3 plants. Mefenpyr treatment also partially reduced the efficacy of thiencarbazone-methyl or mesosulfuron-methyl on MHR3 or MHR4 plants, respectively. Overall, the GSTs described here are not directly involved in enhanced rates of fenoxaprop-P-ethyl or imazamethabenz-methyl metabolism in MHR A. fatua. Instead, we propose that the constitutively elevated GST proteins and related enzymes in MHR plants are representative of a larger, more global suite of abiotic stress-related changes.

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Leaf proteome analysis provides insights into the molecular mechanisms of bentazon detoxification in rice

Cited by 17 publications

References 48 publications

Molecular responses of genetically modified maize to abiotic stresses as determined through proteomic and metabolomic analyses

Molecular responses of genetically modified maize to abiotic stresses as determined through proteomic and metabolomic analyses

Gene Expression Related to Oxidative Stress Induced by Herbicides in Rice

Proteomic and biochemical assays of glutathione-related proteins in susceptible and multiple herbicide resistant Avena fatua L.

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