LASSBio-596 protects gastric mucosa against the development of ethanol-induced gastric lesions in mice

Monteiro, Carlos E.S.; Sousa, Johnatan Alisson Oliveira; Lima, Lı́dia Moreira; Barreiro, Eliezer J.; Silva-Leite, Kaira Emanuella Sales da; Carvalho, Cecília Mendes Morais de; Girão, Deysen Kerlla Fernandes Bezerra; Barbosa, André Luiz R.; Souza, Marcellus H.; Soares, Pedro Marcos Gomes

doi:10.1016/j.ejphar.2019.172662

Cited by 8 publications

(13 citation statements)

References 46 publications

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“…In addition, nitric oxide (NO) and prostaglandin E2 (PGE2), which are endogenous molecules, are described as the second major mucosal defense mechanism, regulating the gastric mucosal acidity [ 44 , 45 ]. NO produced by endothelial cells has a direct vasodilator effect, inhibiting platelet aggregation and thrombosis, thus enhancing the blood flow of gastric mucosa [ 46 , 47 ]. Excessive NO produced by iNOS generates gastric mucosal damage and dysfunction [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Wheat Peptides against Ethanol-Induced Gastric Mucosal Lesions in Rats: Vasodilation and Anti-Inflammation

Liu

et al. 2020

Nutrients

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Alcohol consumption increases the risk of gastritis and gastric ulcer. Nutritional alternatives are considered for relieving the progression of gastric mucosal lesions instead of conventional drugs that produce side effects. This study was designed to evaluate the gastroprotective effects and investigate the defensive mechanisms of wheat peptides against ethanol-induced acute gastric mucosal injury in rats. Sixty male Sprague–Dawley rats were divided into six groups and orally treated with wheat peptides (0.1, 0.2, 0.4 g/kgbw) and omeprazole (20 mg/kgbw) for 4 weeks, following absolute ethanol administration for 1 h. Pretreatment with wheat peptides obviously enhanced the vasodilation of gastric mucosal blood vessels via improving the gastric mucosal blood flow and elevating the defensive factors nitric oxide (NO) and prostaglandin E2 (PGE2), and lowering the level of vasoconstrictor factor endothelin (ET)-1. Wheat peptides exhibited anti-inflammatory reaction through decreasing inducible nitric oxide synthase (iNOS) and pro-inflammatory cytokines tumor necrosis factor α (TNF-α), interleukin (IL)-1β, IL-6, and increasing trefoil factor 1 (TFF1) levels. Moreover, wheat peptides significantly down-regulated the expression of phosphorylated nuclear factor kappa-B (p-NF-κB) p65 proteins in the NF-κB signaling pathway. Altogether, wheat peptides protect gastric mucosa from ethanol-induced lesions in rats via improving the gastric microcirculation and inhibiting inflammation mediated by the NF-κB signaling transduction pathway.

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Section: Discussionmentioning

confidence: 99%

Protective Effects of Wheat Peptides against Ethanol-Induced Gastric Mucosal Lesions in Rats: Vasodilation and Anti-Inflammation

Liu

et al. 2020

Nutrients

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“…This supports the theory that ROS generation and ulcer pathogenesis are closely related [ 44 ]. Furthermore, ethanol also induces infiltration of proinflammatory cells and reduces the secretion of protective factors in the mucosa, such as mucus, bicarbonate, and nitric oxide (NO) [ 45 , 46 ].…”

Section: Discussionmentioning

confidence: 99%

Gastroprotective Effects of the Aqueous Extract from Taraxacum officinale in Rats Using Ultrasound, Histology, and Biochemical Analysis

Zanatta

Miorando

Stefller

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Taraxacum officinale F.H. Wigg. belonging to the family Asteraceae is an edible medicinal plant distributed worldwide. This study aimed to determine the gastroprotective effects of aqueous extract of T. officinale (AETo) in rats using ultrasound, histological, and biochemical analyses. In this study, gastric ulceration was induced by ethanol or piroxicam. Rats were then treated with AETo (3, 30, or 300 mg/kg). The area and histological appearance of gastric ulcers were quantified, and histochemical analysis was performed. The activity of AETo on inflammatory and oxidative stress markers was assessed in the ulcerated tissue. In addition, we investigated the thickness of the gastric wall using the ultrasound technique. Moreover, chemical analyses of AETo were performed. In rats with ethanol- or piroxicam-induced ulcers, AETo reduced the ulceration area, elevated mucin level, and the gastroprotective effect was confirmed by histological analysis. The gastroprotective effect was accompanied by increased activities of SOD, CAT, and GST, as well as an increase in GSH level and reduction in MPO activity. Furthermore, AETo reduced the thickness of the gastric wall in rats. Phytochemical analysis of AETo indicated phenolic acids and flavonoids as the main active compounds. In conclusion, the gastroprotective effect of AETo involves reduction in oxidative stress and inflammatory injury and increase in mucin content. This study advances in the elucidation of mechanisms of gastric protection of T. officinale, contributes to the prospection of new molecules gastroprotective, and proposes the ultrasonographic analyses as a new gastroprotective assessment tool in preclinical studies.

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“…In ethanol-induced GU experiments, it is observed that in the negative control animals there is a reduction in mucus production, decrease in pH, and increase in gastric acidity [57]. After dissolving the mucus, ethanol inhibits the protective capacity of the mucosa, increases its permeability (allowing transport of large molecules) and leads to the dissolution of lipoproteins in the cell membrane [42,57].…”

Section: Mucus Layermentioning

confidence: 99%

“…The gastric epithelium is formed by a continuous layer of narrow junctions cells with secretory and digestive functions [57,58]. The main cells that the infectious agent H. pylori tries to attach are the gastric epithelial cells [59].…”

Section: Gastric Epitheliummentioning

confidence: 99%

“…In gastric injury, inflammatory cytokines IL-1β, IL-6, IL-8, IL-10, TNF-α, and the transcription factor NF-kB-p65 are present [70,71,94,106,107]. IL-1β and TNF-α are increased in ethanol-induced gastric ulcers [57]. IL-1β is considered a hereditary factor for gastric cancer; and, its reduction together with the reduction of TNF-α contributes to the restoration of the gastric mucosa [57,99].…”

Section: Immune Systemmentioning

confidence: 99%

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Gastroprotective Mechanisms

Lima¹,

Lima²,

Souza³

et al. 2022

Peptic Ulcer Disease - What's New?

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Gastric ulcer (GU), a common type of peptic ulcer, results from an imbalance in the action of protective and aggressive agents. Gastroprotective mechanisms are mucus layer, gastric epithelium, gastric blood flow, gastric neurons, mucosal repair capacity, and immune system. Thus, the aim of this chapter was to provide an update on gastroprotective mechanisms. It was carried out through searches in PubMed covering the years 2016–2021 using several keywords. This survey resulted in 428 articles, of which 110 were cited in this chapter. It was reviewed the status of gastroprotective mechanisms and highlighted that mucins can act as a filter; gastric epithelial defenses are composed of the cell barrier, stem cells, and sensors on the mucosal surface; nitric oxide (NO) and hydrogen sulfide (H2S) act for gastric blood flow homeostasis (GBF); the main effector neurons in the gastric mucosa are cholinergic, nitrergic and VIPergic, and oxytocin can activate neurons; repair of the gastric mucosa requires complex biological responses; the immune system regulates the entry of antigens and pathogens. The main knowledge about gastroprotective mechanisms remains unchanged. However, we conclude that there has been progressing in this area.

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LASSBio-596 protects gastric mucosa against the development of ethanol-induced gastric lesions in mice

Cited by 8 publications

References 46 publications

Protective Effects of Wheat Peptides against Ethanol-Induced Gastric Mucosal Lesions in Rats: Vasodilation and Anti-Inflammation

Protective Effects of Wheat Peptides against Ethanol-Induced Gastric Mucosal Lesions in Rats: Vasodilation and Anti-Inflammation

Gastroprotective Effects of the Aqueous Extract from Taraxacum officinale in Rats Using Ultrasound, Histology, and Biochemical Analysis

Gastroprotective Mechanisms

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