Gastroprotective Effects of the Aqueous Extract from Taraxacum officinale in Rats Using Ultrasound, Histology, and Biochemical Analysis

Zanatta, Maria Eduarda D. C.; Miorando, Daniela; Stefller, Amanda M.; Roos, Nátali; Ernetti, Jackeline; Predebon, Ana Júlia; Lindemann, Heloísa; Mânica, Aline; Oliveira, Beatriz M. M.; Serpa, Patrícia Zanotelli; Bohnen, Lilian Caroline; Simomura, Viviane Lazari; Gomes, Denise B.; Vidal-Gutiérrez, Max; Vilegas, Wagner; Silva, Luísa Mota da; Junior, Walter Antônio Roman

doi:10.1155/2021/8987232

Cited by 10 publications

(5 citation statements)

References 74 publications

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“…(2021) reported that the aqueous extract of Taraxacum officinale increased the levels of SOD, CAT, GSH and GST in rats and decreased the levels of myeloperoxidase. The same researchers stated that Taraxacum officinale extract can also prevent the thinning of the gastric wall by decreasing the ulcerative areas and increasing the mucin level [20]. In this study, it was determined that indomethacin caused significant damage to the stomach tissue, whereas Taraxacum officinale leaf ethanol extract, given orally to rats for 10 days before indomethacin administration, reduced the formation of ulcerative areas just like a standard drug, omeprazole.…”

Section: Discussionmentioning

confidence: 85%

Gastroprotective effects of Taraxacum officinale (Dandelion) extract indomethacin-induced gastric ulcer model

Uslu¹,

Uslu²

2022

GSC Biol. Pharm. Sci.

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In this study, it was aimed to investigate the gastroprotective and anti-inflammatory effects of the extract (TO) obtain from the leaves of Taraxacum officinale, which is used in the treatment of many diseases including gastric ulcers in traditional medicine, in an indomethacin-induced gastric ulcer model. Forty rats, which 10 animals in each group, were divided into four groups (Control (C), TO + Indomethacin (TO+IND), Indomethacin (IND) and Omeprazole + Indomethacin (O+IND) groups). Respectively the group C was given isotonic saline, the group TO+IND was given 100 mg/kg TO extract, group IND was given isotonic saline and the group O+IND was given 5 mg/kg omeprazole orally. On the 11th day of the study, a single dose of 100 mg/kg indomethacin was administered orally to the TO+IND, IND and O+IND groups. It was determined that cyclooxygenase-2 (COX-2), C-reactive protein (CRP) and Interleukin-6 (IL-6) levels were significantly increased in the IND group compared to the K group (p<0.001). However, these markers were significantly decreased in both the TO+IND (respectively p<0.05, p<0.001, p<0.05) and O+IND (p<0.05, p<0.001, p<0.001, respectively) groups when compared with the IND group. No significant changes were observed in tumor necrosis factor-alpha (TNF-α) levels. Depending on the administration of indomethacin, it was found that the ulcer index of gastric tissue increased significantly compared to the C group but it decreased significantly as a result of TO and Omeprazole administrations (p<0.001). As a result, it was determined that Taraxacum officinale leaf extract showed significant gastroprotective effects by reducing COX-2, CRP and IL-6 levels and ulcerative area formation in gastric tissue.

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Section: Discussionmentioning

confidence: 85%

Gastroprotective effects of Taraxacum officinale (Dandelion) extract indomethacin-induced gastric ulcer model

Uslu¹,

Uslu²

2022

GSC Biol. Pharm. Sci.

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“…The slices were analyzed by an independent pathologist in a blind manner. Further, the remaining histological slides were used for histochemical analysis of mucins using the periodic acid Schiff (PAS) method, which stains glycoproteins like mucins in pink, as earlier performed [ 34 ].…”

Section: Methodsmentioning

confidence: 99%

Sonchus oleraceus L. Promotes Gastroprotection in Rodents via Antioxidant, Anti-Inflammatory, and Antisecretory Activities

Vecchia

Locateli

Serpa

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Sonchus oleraceus L. is an edible and medicinal plant used to treat stomachache and gastric ailments around the world. Thus, this study aimed to determine the gastroprotective mode of action of hydroalcoholic extract of S. oleraceus (HES). Mice were treated with HES before induction of gastric ulceration by ethanol/HCl. The area and histological appearance of ulcers were quantified, and mucus was measured histochemically. The effects of HES on inflammatory and oxidative markers were assessed in the ulcerated tissue. In addition, we investigated the gastric acid antisecretory activity of HES in pylorus-ligated rats. Chemical analyses of HES and its antioxidant activity were also performed in vitro. The HES (30 or 300 mg/kg) reduced the ulceration by 71.5 and 76.2%, respectively, compared with vehicle ( p < 0.001 ), and the histological analysis confirmed the macroscopic results with elevation in mucin levels by 361.4 and 477.5%, respectively, compared with vehicle ( p < 0.001 ). Moreover, the gastroprotection was accompanied by increases in GSH levels and in SOD, CAT, and GST activities; in parallel to a reduction in MPO activity and TNF levels. Furthermore, HES reduced the total acidity, and pepsin activity of the gastric juice of rats by 61 and 63%, respectively, compared to the vehicle. Phytochemical analysis indicated that luteolin-7-O-β-D-glucoside is the main active compound annotated in HES. Was also found that HES scavenged the DPPH radical with an IC50 of 15.41 μg/mL. In conclusion, the gastroprotective effects of HES involve reductions in oxidative stress and inflammatory injury, in conjunction with an increase in mucus layer and inhibition of gastric secretion. This study advances in elucidating the modes of the antiulcer potential of S. oleraceus and contributes to the prospection of new gastroprotective molecules.

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“…Food was withdrawn 12 h before the experiments and water was provided ad libitum. Several studies performed in female rodents at different stages of the estrous cycle have shown no interference in the results [ 40 , 41 ]. The experiments were conducted following ARRIVE guidelines and the National Council for Animal Experimentation (CONCEA) after approval by the Institutional Animal Ethics Committee of Unochapecó (protocol number 014/18).…”

Section: Methodsmentioning

confidence: 99%

Involvement of Anti-Inflammatory and Stress Oxidative Markers in the Antidepressant-like Activity of Aloysia citriodora and Verbascoside on Mice with Bacterial Lipopolysaccharide- (LPS-) Induced Depression

Gomes

Serpa

Miorando

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Aloysia citriodora Palau is popularly used to treat nervous disorders. Experimental evidence has indicated that verbascoside (VBS) isolated from A. citriodora has pharmacological potential. In this study, we evaluated the antidepressant-like effects of a hydroalcoholic extract of A. citriodora (HEAc) and VBS against lipopolysaccharide- (LPS-) induced depressive-like behavior in mice. In the pretreatment protocol (performed to evaluate the preventive potential), mice were pretreated with HEAc (3, 30, or 300 mg/kg) or VBS (30 mg/kg) before the administration of LPS. In the posttreatment protocol (performed to evaluate the therapeutic potential), mice were initially administered LPS and were subsequently given HEAc (3, 30, or 300 mg/kg) or VBS (30 mg/kg). In both treatments, the mice were submitted to an open-field test and tail suspension test (TST) at 6 and 24 h after LPS administration. The posttreatment evaluation revealed that HEAc (30 or 300 mg/kg) and VBS produced an antidepressant-like effect, as indicated by a reduction in the time spent with no movement in the TST. Moreover, HEAc (30 or 300 mg/kg) was found to reduce interleukin-6 (IL-6) levels and N-acetyl-glycosaminidase activity in the hippocampus, increase glutathione (GSH) levels in the hippocampus and cortex, and enhance IL-10 in the cortex and, at a dose of 300 mg/kg, reduced myeloperoxidase activity in the cortex. Contrastingly, no comparable effects were detected in mice subjected to the pretreatment protocol. Administration of VBS similarly reduced the levels of IL-6 in the hippocampus and increased GSH levels in the cortex. Our observations indicate that both HEAc and VBS show promising antidepressant-like potential, which could be attributed to their beneficial effects in reducing neuroinflammatory processes and antioxidant effects in the central nervous system.

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Gastroprotective Effects of the Aqueous Extract from Taraxacum officinale in Rats Using Ultrasound, Histology, and Biochemical Analysis

Cited by 10 publications

References 74 publications

Gastroprotective effects of Taraxacum officinale (Dandelion) extract indomethacin-induced gastric ulcer model

Gastroprotective effects of Taraxacum officinale (Dandelion) extract indomethacin-induced gastric ulcer model

Sonchus oleraceus L. Promotes Gastroprotection in Rodents via Antioxidant, Anti-Inflammatory, and Antisecretory Activities

Involvement of Anti-Inflammatory and Stress Oxidative Markers in the Antidepressant-like Activity of Aloysia citriodora and Verbascoside on Mice with Bacterial Lipopolysaccharide- (LPS-) Induced Depression

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