Lanthanide Phosphate Nanoparticle-Based One-Step Optical Discrimination of Alkaline Phosphatase Activity

Abstract

Utilizing the dramatic fluorescence difference between the P 2 O 7 :Ce,Tb and PO 4 :Ce,Tb nanoparticles, we proposed the onestep optical strategy for fast, sensitive identification of alkaline phosphatase (ALP) in the present work. In this strategy, the pyrophosphate (PPi) combined with the rare earth ions Ce 3+ , Tb 3+ for its high affinity to metal ions to generate P 2 O 7 :Ce,Tb nanoparticles, which exhibit the bright characteristic green emission of Tb 3+ , it origins from the efficient energy transfer from Ce 3+ to Tb 3+ , the whole process can almost be finished instantaneously. While in the presence of ALP, the enzyme substrate PPi can be broken down into PO 4 3− , which can also self-assemble with Ce 3+ , Tb 3+ ions to produce PO 4 :Ce,Tb nanoparticles, but with a much weaker green light of Tb 3+ , and the greater ALP concentration, the weaker green light can be obtained. Based on this, we proposed a facile optical platform for ALP sensing, compared with those reported probes, it avoids the burdensome process of nanoparticle synthesis and target measurement. The experiment result shows that the fluorescence intensity of P 2 O 7 :Ce,Tb at 545 nm is linear to the concentration of ALP in a range of 0.25−250 U/L, and the detection limit is calculated to be 0.18 U/L. In addition, the excellent selectivity of the present strategy has been confirmed as well; the ALP activity suppression and the real serum sample test demonstrate its outstanding reliability forcibly.

Detection of Periodontal Disease Marker with Geometrical Transformation of Ag Nanoplates

“…Numerous analytics have been developed for the ALP and IL-1β detection including surface-enhanced Raman spectroscopy, − fluorometric, − colorimetric, − electrochemical, ,− and electrical sensors. − Among them, the colorimetric sensors have proven to be most powerful and effective analytics for early diagnosis with simple and fast quantitative analytical performance based on a reduced instrumentation complexity, enabling them interfaced with a point-of-care (POC) assay. , The colorimetric signaling mechanism of the ALP assay is based on the chromogenic signal difference between the phosphate and the de-phosphated species . Ascorbic acid phosphate (AAP) has been utilized as a specific substrate to initiate the chromogenic signal in the ALP assay. − However, this is fundamental limitation for accurate diagnosis because AAP is being used as therapeutic elements to enhance the osseointegration of dental implants and improve post-surgical periodontal healing, forming baseline interfering signal of the periodontal analytes and prohibiting the accurate ALP quantification. − Therefore, it is critically required to design a signaling pathway that can produce diverse signal readout based on colorimetric responses for achieving multivariate analysis, high-throughput diagnosis, and versatility in dental applications.…”

Ultrarapid Microwave-Assisted Synthesis of Fluorescent Silver Coordination Polymer Nanoparticles and Its Application in Detecting Alkaline Phosphatase Activity

“…In biological systems, the ALP activity is closely associated with the health status of living organisms. ALP contained in serum is an important biomarker for several diseases, including liver dysfunction, leukemia, breast and prostatic cancers, diabetes, and bone and kidney diseases [ 4 , 5 ]. Therefore, it is of great significance for biomedical diagnoses to construct a simple, sensitive, and accurate biosensor to monitor ALP activity.…”

Glucose oxidase-encapsulated liposomes for amplified autofluorescence-free immunoassay of a prostate-specific antigen with photoluminescence of CePO4:Tb nanocrystals

“…3,15 Some trivalent lanthanide (Ln 3+ ) doped inorganic nanoparticles (NPs) possess fascinating optical properties, such as strong stability, high photoluminescence efficiency, a large Stokes shift, and a long luminescence lifetime, which bring hope to avoid the shortcomings of traditional fluorescent nanoprobes. [16][17][18] The green luminescence of Tb 3+ is typical long-lived photoluminescence, generally above the millisecond level, which can be clearly distinguished from the autofluorescence in complex biological matrices. 19,20 Currently, most of the autofluorescence-free biosensors constructed from Tb 3+ doped inorganic nanoparticles are based on the time-resolved fluorescence resonance energy transfer principle.…”