Lanostane Triterpenes Isolated from <i>Antrodia heteromorpha</i> and Their Inhibitory Effects on RANKL-Induced Osteoclastogenesis

Kwon, Jae‐Young; Lee, Hyaemin; Yoon, Yeo Dae; Hwang, Bang Yeon; Guo, Yuanqiang; Kang, Jong Soon; Kim, Jae Jin; Lee, Dong‐Ho

doi:10.1021/acs.jnatprod.6b00207

Cited by 16 publications

(22 citation statements)

References 21 publications

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“…All the constituents identified in our present study were in agreement with those identified in previous reports [9,16,26,27]. Polyporenic acid C inhibits M-CSF and RANKL-induced osteoclastogenesis in BMMs [28], while pachymic acid enhances odontoblast differentiation and dentin mineralization via induction of heme oxygenase-1 [29]. The other chemicals identified in this study have not, thus far, been associated with bone metabolism.…”

Section: Phytochemical Constituents Of Pcsupporting

confidence: 92%

Poria Cocos Ameliorates Bone Loss in Ovariectomized Mice and Inhibits Osteoclastogenesis In Vitro

et al. 2020

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Estrogen deprivation in postmenopausal women causes disruption of bone homeostasis, resulting in bone loss and osteoporosis. Conventional therapies can exert adverse effects. The sclerotum of Poria cocos has been used in traditional medicine and as a nutritional supplement and to treat various diseases. However, the effects of P. cocos on the bone remain largely undetermined. In this study, we examined the effects of P. cocos hydroethanolic extract (PC) on osteoclast differentiation and estrogen-deprivation-induced bone loss in an ovariectomized mouse model of postmenopausal osteoporosis. PC-mediated inhibition of receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast formation and resorption activity suppressed RANKL-induced expression of nuclear factor of activated T cells cytoplasmic 1 (NFATc1), which is a crucial transcription factor for osteoclast differentiation. In ovariectomized mice, PC markedly alleviated trabecular bone loss and reduced the accumulation of lipid droplets in the bone marrow. We additionally identified ten triterpenoid constituents of PC using UPLC-MS/MS analysis. Our results indicate that PC negatively regulated osteoclast differentiation and function, and can potentially be used to manage postmenopausal osteoporosis.

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Section: Phytochemical Constituents Of Pcsupporting

confidence: 92%

Poria Cocos Ameliorates Bone Loss in Ovariectomized Mice and Inhibits Osteoclastogenesis In Vitro

et al. 2020

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“…All 13 conformers which had Boltzmann-averaged weight less than 0.95 were selected and optimized using theoretical method of density functional theory (B3LYP) and a basis set of 6-31 + G (d,p) in Gaussian 16 software (Gaussian Inc., Wallingford, CT, USA) in gas phase. Finally, their calculated ECD was carried out using TDDFT with the CAM-B3LYP/6-31 + G (d,p) method and the methanol-selected CPCM in Gaussian 16 software [15,16]. Lastly, the calculated ECD curves of possible structures (1a and 1b) were compared with the experimental ECD curves (Fig.…”

Section: Calculated Ecd Predictionmentioning

confidence: 99%

Chemical Constituents with Inhibitory Activities on Proprotein Convertase Subtilisin/kexin Type 9 Expression from the Aerial Parts of Penthorum chinense

Pel¹,

Chae²,

Cho³

et al. 2020

Preprint

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Background: Penthorum chinense has been used in East Asia for the treatment of cholecystitis, infectious hepatitis, and jaundice. So far there is no report regarding proprotein convertase subtilisin/kexin type 9 inhibitory constituents from this plant. The aim of the present study was to discover new active constituents with PCSK9 expression inhibitory activities from P. chinenseMethods: All structures were established by interpreting NMR spectroscopic data and MS data. Further experimental and calculated ECD data were used to determine the absolute configuration of the two new neolignans. To monitor the inhibitory activity on proprotein convertase subtilisin/kexin type 9 (PCSK9) mRNA expression and PCSK9-low density lipoprotein receptor (LDLR) interaction, quantitative real time-PCR, Western blot analysis, and an enzyme-linked immunosorbent assay (ELISA) method by a PCSK9-biotinylated-LDLR binding assay were performed.Results: 39 compounds were isolated and identified including two new oxepine-type neolignans, penthorinols A (1) and B (2) and a naturally occurring chalcone, 6'-hydroxy-2'-methoxychalcone-4'-O-β-D-glucopyranoside (20). Of all tested compounds, penthorin A (4) and methyl gallate (25) were found to suppress PCSK9 mRNA expression with IC50 values of 15.56 and 11.66 µM, respectively. Furthermore, penthorin A (4) and methyl gallate (25) downregulated PCSK9 protein expression. However, all compounds seemed to be inactive in PCSK9-LDLR interaction.Conclusion: In the present study, two new compounds was discovered from this plant and active constituents with PCSK9 expression inhibitory activities were suggested.

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“…Astersaponins are known to exert antitumor, expectorant, and antitussive activities [22], while no autophagy inducing effects of astersaponins or conyzasaponins in tumor cells or neuronal cells have been reported. Furthermore, there is no previous literature on astersaponins isolated from Aster koraiensis.…”

Section: Bioactivities Of Atersaponin Imentioning

confidence: 99%

“…Furthermore, there is no previous literature on astersaponins isolated from Aster koraiensis. Astersaponins have been mainly reported from Aster tataricus [22]. Astersaponin I is the first reported saponin from A. koraiensis.…”

Section: Bioactivities Of Atersaponin Imentioning

confidence: 99%

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An Autophagy Inducing Triterpene Saponin Derived from Aster koraiensis

Kwon

Zhang

et al. 2019

Molecules

Self Cite

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Autophagy is an important self-degradative mechanism that plays a key role in treating neurodegeneration diseases. This research aimed at discovering bioactive compounds from Aster koraiensis. A new triterpene saponin, astersaponin I (1), was isolated from the EtOH extract of A. koraiensis. The structure of 1 was characterized by spectroscopic methods, ECD calculation, and acid hydrolysis. The biochemical analysis showed that compound 1 significantly increased the expression of microtubule-associated protein 1A/1B light chain 3B (LC3-II) expression in SH-SY5Y cells, which indicates the induction of autophagy. Thus, further study may be needed to clarify whether compound 1 exerts beneficial effects on neurodegeneration diseases like Parkinson’s disease through autophagy induction.

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Lanostane Triterpenes Isolated from Antrodia heteromorpha and Their Inhibitory Effects on RANKL-Induced Osteoclastogenesis

Cited by 16 publications

References 21 publications

Poria Cocos Ameliorates Bone Loss in Ovariectomized Mice and Inhibits Osteoclastogenesis In Vitro

Poria Cocos Ameliorates Bone Loss in Ovariectomized Mice and Inhibits Osteoclastogenesis In Vitro

Chemical Constituents with Inhibitory Activities on Proprotein Convertase Subtilisin/kexin Type 9 Expression from the Aerial Parts of Penthorum chinense

An Autophagy Inducing Triterpene Saponin Derived from Aster koraiensis

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