Poria Cocos Ameliorates Bone Loss in Ovariectomized Mice and Inhibits Osteoclastogenesis In Vitro

Abstract

Estrogen deprivation in postmenopausal women causes disruption of bone homeostasis, resulting in bone loss and osteoporosis. Conventional therapies can exert adverse effects. The sclerotum of Poria cocos has been used in traditional medicine and as a nutritional supplement and to treat various diseases. However, the effects of P. cocos on the bone remain largely undetermined. In this study, we examined the effects of P. cocos hydroethanolic extract (PC) on osteoclast differentiation and estrogen-deprivation-induced bone loss in an ovariectomized mouse model of postmenopausal osteoporosis. PC-mediated inhibition of receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast formation and resorption activity suppressed RANKL-induced expression of nuclear factor of activated T cells cytoplasmic 1 (NFATc1), which is a crucial transcription factor for osteoclast differentiation. In ovariectomized mice, PC markedly alleviated trabecular bone loss and reduced the accumulation of lipid droplets in the bone marrow. We additionally identified ten triterpenoid constituents of PC using UPLC-MS/MS analysis. Our results indicate that PC negatively regulated osteoclast differentiation and function, and can potentially be used to manage postmenopausal osteoporosis.

Palmul-Tang, a Korean Medicine, Promotes Bone Formation via BMP-2 Pathway in Osteoporosis

“…In the previous study, P. ginseng, P. lactiflora and L. officinale, consists of Palmul-tang, is recently reported to enhance the osteoblast activities, leading to generation of new bone matrix (Yen et al, 2007;Kim et al, 2013;Dong et al, 2020). In addition, A. macrocephala and P. cocos, and decursin derived from A. gigas are known to ameliorate the development progress of osteoporosis by inhibiting osteoclast differentiation (Ha et al, 2013;Wang et al, 2016c;Hwang et al, 2020). Moreover, G. uralensis and R. glutinosa showed protective effects on osteoporosis by maintaining bone mineral density (Lim and Kim, 2013;Galanis et al, 2019).…”

“…In addition, A . macrocephala and P. cocos , and decursin derived from A. gigas are known to ameliorate the development progress of osteoporosis by inhibiting osteoclast differentiation ( Ha et al, 2013 ; Wang et al, 2016c ; Hwang et al, 2020 ). Moreover, G. uralensis and R. glutinosa showed protective effects on osteoporosis by maintaining bone mineral density ( Lim and Kim, 2013 ; Galanis et al, 2019 ).…”

A Herbal Mixture Formula of OCD20015-V009 Prophylactic Administration to Enhance Interferon-Mediated Antiviral Activity Against Influenza A Virus

“…Data acquisition and processing were performed using Xcalibur v.3.0 and Tracefinder v.4.0. Chromatographic separation was achieved with an Acquity BEH C18 column (100 mm × 2.1 mm, 1.7 μm, Waters, United States) with gradient elution consisting of 0.1% formic acid in water and acetonitrile ( Hwang et al, 2019 ; Hwang et al, 2020 ). The identified compounds were compared to the retention time and mass spectrum of the authenticated standards.…”

Normalisation of High Bone Remodelling due to Oestrogen Deficiency by Traditional Chinese Formulation Kang Shuai Lao Pian in Ovariectomised Rats

“…[9]. Each of these ingredients has been previously demonstrated to protect against osteoporosis and/or improve oxidative stress in preclinical studies [10][11][12][13][14][15]. A preliminary study has suggested that KSLP promoted osteogenic differentiation of adipose-derived stem cells in aged mice [16].…”