Laccase Production and Humic Acids Decomposition by Microscopic Soil Fungi

Заварзина, А. Г.; Семенова, Т. А.; Belova, O. V.; Lisov, A. V.; Leontievskii, A. A.; Иванова, А. Е.

doi:10.1134/s0026261718030153

Cited by 8 publications

(4 citation statements)

References 26 publications

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“…Streptomyces is commonly used in industrial applications to produce laccase and xylanase, using rice or wheat straw as substrates (55,56). Microbial laccase production is usually related to the ability of the microbe to degrade lignin (57), and lignin is also the most difficult decomposable straw component. The higher abundance of Streptomyces in NPK than in NPKM soil may be related to the higher content of recalcitrant components in NPK soil resulting from the 3-year application of mineralonly fertilizers (58).…”

Section: Discussionmentioning

confidence: 99%

DNA Stable-Isotope Probing Delineates Carbon Flows from Rice Residues into Soil Microbial Communities Depending on Fertilization

Kong

Kuzyakov

Ruan

et al. 2020

Appl Environ Microbiol

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Decomposition of crop residues in soil is mediated by microorganisms whose activities vary with fertilization. The complexity of active microorganisms and their interactions utilizing residues is impossible to disentangle without isotope applications. Thus, 13 C-labeled rice residues were employed, and DNA stable-isotope probing (DNA-SIP) combined with high-throughput sequencing was applied to identify microbes active in assimilating residue carbon (C). Manure addition strongly modified microbial community compositions involved in the C flow from rice residues. Relative abundances of the bacterial genus Lysobacter and fungal genus Syncephalis were increased, but abundances of the bacterial genus Streptomyces and fungal genus Trichoderma were decreased in soils receiving mineral fertilizers plus manure (NPKM) compared to levels in soils receiving only mineral fertilizers (NPK). Microbes involved in the flow of residue C formed a more complex network in NPKM than in NPK soils because of the necessity to decompose more diverse organic compounds. The fungal species (Jugulospora rotula and Emericellopsis terricola in NPK and NPKM soils, respectively) were identified as keystone species in the network and may significantly contribute to residue C decomposition. Most of the fungal genera in NPKM soils, especially Chaetomium, Staphylotrichum, Penicillium, and Aspergillus, responded faster to residue addition than those in NPK soils. This is connected with the changes in the composition of the rice residue during degradation and with fungal adaptation (abundance and activity) to continuous manure input. Our findings provide fundamental information about the roles of key microbial groups in residue decomposition and offer important cues on manipulating the soil microbiome for residue utilization and C sequestration in soil. IMPORTANCE Identifying and understanding the active microbial communities and interactions involved in plant residue utilization are key questions to elucidate the transformation of soil organic matter (SOM) in agricultural ecosystems. Microbial community composition responds strongly to management, but little is known about specific microbial groups involved in plant residue utilization and, consequently, microbial functions under different methods of fertilization. We combined DNA stable-isotope ( 13 C) probing and high-throughput sequencing to identify active fungal and bacterial groups degrading residues in soils after 3 years of mineral fertilization with and without manure. Manuring changed the active microbial composition and complexified microbial interactions involved in residue C flow. Most fungal genera, especially Chaetomium, Staphylotrichum, Penicillium, and Aspergillus, responded to residue addition faster in soils that historically had received manure. We generated a valuable library of microorganisms involved in plant residue utilization for future targeted research to exploit spe-

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Section: Discussionmentioning

confidence: 99%

DNA Stable-Isotope Probing Delineates Carbon Flows from Rice Residues into Soil Microbial Communities Depending on Fertilization

Kong

Kuzyakov

Ruan

et al. 2020

Appl Environ Microbiol

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“…Arthrobacter , which was newly categorised as a key taxon in the bacterial network of biochar‐amended soils (Chen, Jiang, et al, 2019) and an important plant growth‐promoter (Chen, Waghmode, et al, 2019; Fan et al, 2014), has competitive advantages for breaking down recalcitrant C components and efficiently utilising limited resources. Streptomyces with ability to degrade the most recalcitrant lignin is commonly used in commercial processes to produce laccase and xylanase using crop straw as substrates (Berrocal et al, 2000; Niladevi et al, 2007; Zavarzina et al, 2018). Catenulispora , which is capable of lignocellulosic material degradation and can produce secondary metabolites for utilisation of other microbes, was also continuously present in the early decomposition stage in our studies (Busti et al, 2006; Copeland et al, 2009; Guo et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Continuous straw return for 8 years mitigates the negative effects of inorganic fertilisers on C‐cycling soil bacteria

Zhang

Guo

Sheng

et al. 2022

European J Soil Science

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To elucidate the identity and mechanisms by which specific bacterial members drive efficient decomposition and utilisation of straw, the succession and co‐occurrence patterns of 13C‐labelled bacteria were analysed using DNA‐stable isotope probing (SIP) techniques. Microcosms with 13C‐labelled maize straw incorporated into soils subjected to no nitrogen (N) fertiliser (CK), chemical fertiliser (NPK) and chemical fertiliser with straw return (NPKS) treatments for 8 years were incubated for 360 days. The results showed that fertilisation regimes changed the 13C‐labelled bacterial groups by abundance but not taxonomic composition, where N fertiliser application (NPK and NPKS treatments) reduced the relative abundance of maize straw carbon assimilators affiliated with Arthrobacter, Sphingomonas, Streptomyces, Burkholderia‐Caballeronia‐Paraburkholderia and Kribbella, but increased the relative abundance of Nocardioides, Rhodanobacter, Granulicella and Chujaibacter. Compared with CK and NPKS‐treated soils, continuous inorganic fertilisation reduced the α‐diversity of the 13C‐labelled bacterial community and resulted in a loose and less competitive co‐occurrence network of straw C‐assimilators with more hubs needed to sustain its structural stability. Interestingly, straw return mitigated the negative effects of inorganic fertilisation by forming a tight and complex co‐occurrence network of maize straw utilizers associated with NPKS‐treated soils. Taken together, we emphasise the vital function of long‐term straw return for sustaining soil biodiversity under intensive fertilised agricultural conditions and provide reference bacterial members for efficient straw utilisation. Highlights DNA‐SIP enabled the concise identification of straw C assimilating bacterial members. Inorganic fertilisation reduced straw C‐utilizer community diversity and their interactions. Straw return mitigates the negative effects of inorganic fertilisers on straw C‐cycling bacteria. Varying taxa sustained the interaction network of straw C‐utilizers in different fertilised soils.

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“…The work of the enzyme leads to the formation of phenoxy radicals and quinones, which initiate spontaneous reactions, including the breaking of C-C bonds in aromatic substrates, their demethoxylation, demethylation and polymerization, as well as the formation of reactive oxygen species. Unlike ligninolytic peroxidases, which are characteristic exclusively of white rot basidiomycetes and their litter fungi, laccase is also produced by ascomycetes, deuteromycetes, bacteria and is the most common oxidative enzyme in soils [14].…”

Section: Fig 1 the Structure Of Ligninmentioning

confidence: 99%

“…Laccase Gray forest soil and black soil Humic acids have a mixed effect on laccase products [14] Fusarium culmorum, Fusarium sporotrichioides, Fusarium solani and Trametes versicolor Laccase, Mn-Peroxidase, lignin peroxidase Forest soil and tree bark Strains secreted laccase, manganese peroxidase and lignin peroxidase into the growth medium under butch cultures; the yield of enzymes in the culture medium averaged from 75.5% to 91.9%, which facilitates their isolation and purification [19] Anoxybacillus sp. strain UARK-01…”

Section: Acremonium Murorum and Botrytis Cinereamentioning

confidence: 99%

Biotechnological potential of fungi and bacteria with ligninolytic activity (mini-review)

2021

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Laccase Production and Humic Acids Decomposition by Microscopic Soil Fungi

Cited by 8 publications

References 26 publications

DNA Stable-Isotope Probing Delineates Carbon Flows from Rice Residues into Soil Microbial Communities Depending on Fertilization

DNA Stable-Isotope Probing Delineates Carbon Flows from Rice Residues into Soil Microbial Communities Depending on Fertilization

Continuous straw return for 8 years mitigates the negative effects of inorganic fertilisers on C‐cycling soil bacteria

Biotechnological potential of fungi and bacteria with ligninolytic activity (mini-review)

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