Laboratory diagnosis of mycoplasma infection in young cattle

Abstract

The aim of this study was to detect Mycoplasma species in the respiratory tract of 110, 310 and 510 day-old groups of cattle by serological, bacteriological and histopathological investigations. Antibodies against M. bovis were found in 75% of the 110 day-old, in 50% -of the 310 day-old and in 55% -of the 510 day-old groups of cattle.Bacteriological examination of the samples from nasal cavities revealed that Mycoplasma carriers were found in 60% of the 110 day-old group of cattle, 40% of the 310 day-old and 40% of the 510 day-old group of cattle. Using the PCR method Mycoplasma was isolated from 25% of lung samples of the 510 day-old group of cattle. Mycoplasma bovis and Mycoplasma dispar were confirmed by serological investigations. Foci of bronchointerstitial pneumonia were determined by histopathological examination in 27.5% of lung samples. Mycoplasma bovis was isolated in 72.7% of bronchointerstitial pneumonia cases. Data processing with an SPSS 13.0 statistical package led to the conclusion that Mycoplasma bovis was found more frequently in the 110 day-old group of cattle (the youngest age group in this study) rather than in the 310 and 510 day-old groups of cattle (χ 2 = 6.531; p = 0.038). The results obtained led to the conclusion that serological, bacteriological and histopathological examinations are important in detecting particular animal -carriers of Mycoplasma.

Susceptibility of Mycoplasma bovis field isolates to antimicrobial agents

“…DNA from isolated microorganisms was extracted with a 5% Chelex solution (Sigma, USA). Isolated microorganisms were analyzed by PCR using the forward primer, MW28 (5'-CCAGACTCCTACGGGAGGCA-3') and reverse oligonucleotide primer MW29 (5'-T GCGAGCATACTACTCAGGC-3') (Grida Lab, Lithuania) that are specific for the Mollicutes class (Bashiruddin et al 2005;Gabinaitiene et al 2011). This primer pair generates a 560 bp product.…”

“…M. bovis is also present in the respiratory tract of healthy cattle where it causes a subclinical upper respiratory infection and may provoke mastitis and metritis in caws and arthritis or tenosinovitis in fattening cattle (Fox et al 2005;Gagea et al 2006). From 2007 through to 2009, studies in Lithuania revealed that M. bovis was present in the upper respiratory tract of 52.5 percent (%) of healthy cattle (Gabinaitiene et al 2011). …”

Evaluation ofMollicutesMicroorganisms in Respiratory Disease of Cattle and Their Relationship to Clinical Signs

“…12 After stressful situations, M. bovis becomes pathogenic and clinical signs of BRD are observed, especially in young calves. 13 M. dispar was first isolated from pneumonic lungs of cattle, 14 and it has been described as a potential pathogen associated with BRD. 15,16 Considering the importance of Mycoplasma spp.…”

A review of mycoplasma diagnostics in cattle

“…Unpublished manuscript: The University of Sydney, Sydney School of Veterinary Science, Faculty of Science; 2017). Lastly, in a study involving the examination of 3 age groups (110, 310, and 510 days old) of male cattle for M. bovis on a breeding station, a higher percentage of animals were identified as positive by ELISA in serum samples (75, 50, and 55%) than by culture from samples taken from the nasal cavity (52, 30, and 27%) . These studies demonstrate that while the number of animals exposed to M. bovis may be high, the proportion of animals that succumb to disease or are actively shedding the pathogen is frequently much lower.…”