Label-free fluorescent sensor based on aptamer and thiazole orange for the detection of tetracycline

Sun, Chia‐Chung; Su, Ruifang; Bie, Jiaxin; Sun, Hui; Qiao, Shangna; Ma, Xinyue; Sun, Rui; Zhang, Tiehua

doi:10.1016/j.dyepig.2017.11.031

Cited by 66 publications

(42 citation statements)

References 61 publications

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“…Applications of fluorescent aptasensors to the detection of antimicrobial residues in animal-derived food products are presented in Table 1. Label-free fluorescence aptasensors can be based on DNA intercalating dyes (e.g., Ru (phen) 2 (dppz))2+, SYBR Green I [96], thiazole orange (TO) [101], metal nanoparticles (NPs) [109]). The detection is based on the fluorescence signal of intercalating dyes (e.g., TO) that is low in solutions, but increases when the dye intercalates into dsDNA ( Figure 2).…”

Section: Fluorescent Aptasensorsmentioning

confidence: 99%

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The Growing Interest in Development of Innovative Optical Aptasensors for the Detection of Antimicrobial Residues in Food Products

Gaudin¹

2020

Biosensors

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The presence of antimicrobial residues in food-producing animals can lead to harmful effects on the consumer (e.g., allergies, antimicrobial resistance, toxicological effects) and cause issues in food transformation (i.e., cheese, yogurts production). Therefore, to control antimicrobial residues in food products of animal origin, screening methods are of utmost importance. Microbiological and immunological methods (e.g., ELISA, dipsticks) are conventional screening methods. Biosensors are an innovative solution for the development of more performant screening methods. Among the different kinds of biosensing elements (e.g., antibodies, aptamers, molecularly imprinted polymers (MIP), enzymes), aptamers for targeting antimicrobial residues are in continuous development since 2000. Therefore, this review has highlighted recent advances in the development of aptasensors, which present multiple advantages over immunosensors. Most of the aptasensors described in the literature for the detection of antimicrobial residues in animal-derived food products are either optical or electrochemical sensors. In this review, I have focused on optical aptasensors and showed how nanotechnologies (nanomaterials, micro/nanofluidics, and signal amplification techniques) largely contribute to the improvement of their performance (sensitivity, specificity, miniaturization, portability). Finally, I have explored different techniques to develop multiplex screening methods. Multiplex screening methods are necessary for the wide spectrum detection of antimicrobials authorized for animal treatment (i.e., having maximum residue limits).

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Section: Fluorescent Aptasensorsmentioning

confidence: 99%

“…Figure 2. Schematic illustration of the label-free fluorescent probe based on structure-switching aptamers for tetracycline detection [101]. Reprinted from [101]; with permission from Elsevier.…”

Section: Figurementioning

confidence: 99%

The Growing Interest in Development of Innovative Optical Aptasensors for the Detection of Antimicrobial Residues in Food Products

Gaudin¹

2020

Biosensors

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“…Carboxyuorescein (FAM) and other uorescent dyes are commonly used to functionalize DNA and are quenched by the complementary strand labelled with a quenching group. [22][23][24] In addition, a very common and effective approach is to introduce novel nanomaterials (gold and silver nanoclusters, carbon dots (CD) and graphene dots) into uorescent biosensors. 25,26 Due to their adsorption of DNA with appropriate affinity and the ability to quench different types of uorophores, graphene-based nanomaterials can act as unique platforms for uorescence sensing.…”

Section: Dna Biosensorsmentioning

confidence: 99%

Growing prospects of DNA nanomaterials in novel biomedical applications

et al. 2019

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“…Sun et al [222] introduced a label-free fluorescent aptasensor for tetracycline detection based on thiazole orange as intercalated dye, according to the work of Xing et al [83]. Essentially thiazole orange is no fluorescent in aqueous solution, but it shows high fluorescence emission intensity upon interaction with nucleic acids without regard to base composition.…”

Section: Tetracyclinementioning

confidence: 99%

Aptamer-based Biosensors for Antibiotic Detection: A Review

Mehlhorn

Rahimi

Joseph

2018

Preprint

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Antibiotic resistance and accordingly their pollution because of uncontrolled usage has emerged as a serious problem in recent years. Hence, there is an increased demand to develope robust, easy, and sensitive methods for rapid evaluation of antibiotic and their residues. Among different analytical methods, the aptamer-based biosensors (aptasensors) have attracted considerable attention because of good selectivity, specificity, and sensitivity. This review gives an overview about recent developed aptasensors for antibiotic detection. The use of various aptamer assays to determine different groups of antibiotics like β-lactams, Aminoglycosides, Anthracyclines, Chloramphenicol, (Fluoro)Quinolones, Lincosamide, Tetracyclines and Sulfonamides are presented in this paper.

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Label-free fluorescent sensor based on aptamer and thiazole orange for the detection of tetracycline

Cited by 66 publications

References 61 publications

The Growing Interest in Development of Innovative Optical Aptasensors for the Detection of Antimicrobial Residues in Food Products

The Growing Interest in Development of Innovative Optical Aptasensors for the Detection of Antimicrobial Residues in Food Products

Growing prospects of DNA nanomaterials in novel biomedical applications

Aptamer-based Biosensors for Antibiotic Detection: A Review

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