Aptamer-based Biosensors for Antibiotic Detection: A Review

Abstract

Antibiotic resistance and accordingly their pollution because of uncontrolled usage has emerged as a serious problem in recent years. Hence, there is an increased demand to develope robust, easy, and sensitive methods for rapid evaluation of antibiotic and their residues. Among different analytical methods, the aptamer-based biosensors (aptasensors) have attracted considerable attention because of good selectivity, specificity, and sensitivity. This review gives an overview about recent developed aptasensors for antibiotic detection. The use of various aptamer assays to determine different groups of antibiotics like β-lactams, Aminoglycosides, Anthracyclines, Chloramphenicol, (Fluoro)Quinolones, Lincosamide, Tetracyclines and Sulfonamides are presented in this paper.

Challenges and Applications of Impedance-Based Biosensors in Water Analysis

“…A good overview about aptamer-based EIS biosensors to determine different groups of antibiotics in water samples is presented in Ref. [74].…”

Chemical Modification of Aptamers for Increased Binding Affinity in Diagnostic Applications: Current Status and Future Prospects

“…As a consequence, the development of so-called aptasensors has received considerable attention, which has resulted in an extensive variety of detection strategies, ranging from electrochemical, colorimetric to gravimetric detection [ 4 ]. These aptamer-based biosensors use aptamers to recognize and detect various analytes such as cocaine, antibiotics and disease biomarkers, to name just a few [ 5 , 6 , 7 ]. Furthermore, aptamer-linked immobilized sorbent assays (ALISA) and aptamer-based lateral flow immunoassays have been developed [ 8 , 9 ].…”

Silver nanoparticle probe for colorimetric detection of aminoglycoside antibiotics: picomolar‐level sensitivity toward streptomycin in water, serum, and milk samples

“…However, the sensitivity of such agar‐based diffusion methods remains questionable. The residue levels of antibiotics are predominantly detected by high‐performance liquid chromatography, liquid chromatography–mass spectrometry, and gas chromatography–mass spectrometry . Enzyme‐linked immunosorbent assay, fluorescence immunoassay, and radioimmunoassay have also been used to detect streptomycin residues; however, cross‐reactions can prevent efficient determination of the target analyte .…”