Label-free exonuclease I-assisted signal amplification colorimetric sensor for highly sensitive detection of kanamycin

The recent development of nanozymes for food quality and safety detection

“…Due to the widespread application of antibiotics as feed additives in animal husbandry, there are worries that animal-derived food may be contaminated by antibiotics. As an example of an aminoglycoside antibiotic, kanamycin 68 has been widely used in the treatment of infections induced by Gram-positive and Gram-negative bacteria in humans and animals. Through the food chain, kanamycin residue in food may accumulate in the human body, which may induce some serious side effects, such as hearing loss and nephrotoxicity.…”

A smartphone-based gold nanoparticle colorimetric sensing platform for kanamycin detection in food samples

“…Its mechanism of action involves interfering with protein synthesis. 1,2 However, it is important to note that Kana has a limited therapeutic index and excessive usage may result in the presence of its residues in the food chain. This can lead to contamination of both humans and the environment.…”

Optimisation of a gold nanoparticle-based aptasensor integrated with image processing for the colorimetric detection of acephate using response surface methodology

“…33,42,43 Compared to antibodies, aptamers exhibit certain outstanding merits, such as excellent stability, in vitro synthesis, ease of modication and xing, small size, low toxicity, and cost efficiency. 44 Aptamers are single-stranded, synthetic oligonucleotides (DNA or RNA), which fold into 3-dimensional shapes 45,46 and are capable of binding non-covalently and with high affinity to a target molecule. 47,48 They can bind with such specicity that they can differentiate enantiomers and molecules that differ by as little as one functional group.…”

“…45,[50][51][52][53][54][55] Li et al (2021) developed a label-free target binding induced aptamer structure switch colorimetric sensor for ultrasensitive detection of kanamycin based on Exo Iassisted signal amplication and polycationic protamine mediated aggregation of negatively charged AuNPs with a low detection limit of 2.8 Â 10 À14 M under optimal conditions. 44 Besides that, many studies have used AuNPs based aptasensors for the detection of OPs, which resulted in notable low limits of detection (LOD). 23,33,36,[56][57][58][59][60] The detection of an analyte is simple and easy using a colorimetric method; however, there is a concern about detection accuracy, which may arise when the determination of color change is only based on naked eyes.…”

“… 45,50–55 Li et al (2021) developed a label-free target binding induced aptamer structure switch colorimetric sensor for ultrasensitive detection of kanamycin based on Exo I-assisted signal amplification and polycationic protamine mediated aggregation of negatively charged AuNPs with a low detection limit of 2.8 × 10 −14 M under optimal conditions. 44 Besides that, many studies have used AuNPs based aptasensors for the detection of OPs, which resulted in notable low limits of detection (LOD). 23,33,36,56–60 …”