Kıvırcık Irkı Koyunlarda Melatonin Reseptör 1A (MTNR1A) Geninin MnlI ve RsaI Restriksiyon Enzimleri ile Genetik Varyasyonunun Belirlenmesi

Avanus, Kozet; Altınel, Ahmet

doi:10.9775/kvfd.2016.15089

Cited by 2 publications

(2 citation statements)

References 21 publications

(43 reference statements)

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“…However, in the Barki breed, the CC genotype was more frequent. The same result was reported by Giantsis et al (2016) in a local Greek sheep breed, Saxena et al (2015b) in the tropical sheep breeds of India, and Avanus and Altinel (2016) in Kıvırcık breed ewes. The TT homozygous genotype had the lowest frequencies in Ossimi and Rahmani and was not represented in the Barki breed, probably due to the low sample size.…”

Section: Discussionsupporting

confidence: 84%

“…However, melatonin receptor 1A (MTNR1A) is the main receptor that mediates the reproductive and circadian actions of melatonin in sheep. Polymorphisms in the MTNR1A gene have been documented to be associated with seasonal reproduction in various breeds of sheep (Carcangiu et al, 2009a;Ahmad et al, 2015), goat (Agaoglu et al, 2015), pig (Ramírez et al, 2009), deer (Yang et al, 2014) and buffalo (Barbosa et al, 2017). Arylalkylamine N-acetyltransferase (AA-NAT) is called "the Timezyme" because it plays a unique role in vertebrate timekeeping (Klein, 2007) and is considered the rate-limiting enzyme in melatonin biosynthesis.…”

Section: Introductionmentioning

confidence: 99%

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Genetic polymorphism in melatonin receptor 1A and arylalkylamine N-acetyltransferase and its impact on seasonal reproduction in Egyptian sheep breeds

Fathy¹,

Gouda

Gafer³

et al. 2018

Arch. Anim. Breed.

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This study was carried out to detect polymorphisms in the melatonin receptor 1A (MTNR1A) and arylalkylamine N-acetyltransferase (AA-NAT) genes and their association with reproductive traits. Blood samples of 126 animals from three Egyptian sheep breeds were collected. DNA was extracted and subjected to PCR restriction fragment length polymorphism (RFLP) analysis using the RsaI and SmaI enzymes. Two alleles (C and T) and three genotypes (CC, CT and TT) for MTNR1A and for AA-NAT (A and G; GG, GA and AA) were detected. The alleles C and A and the genotypes CT and GA showed the highest frequencies for the MTNR1A and AA-NAT genes, respectively. Association analysis of the MTNR1A single nucleotide polymorphism (SNP) with ewe reproductive traits revealed significant associations in the Ossimi and Rahmani breeds with age at first lambing, and the C allele seemed to be the favorable allele. The results for the AA-NAT SNP demonstrated significant correlations in Ossimi with age at first lambing and litter size and in Rahmani with lambing interval; the G allele seemed to be the desirable allele. In the first conception season, ewes carrying CT exhibited a significantly lower age of first lambing in the unfavorable season. Additionally, GG ewes exhibited a significantly lower age of first lambing in the early favorable season, followed by the unfavorable season. To the best of our knowledge, this is the first study of these associations in Egyptian sheep breeds. In conclusion, the polymorphisms revealed in this study could be used as genetic markers to improve reproductive efficiency during the unfavorable season, and the obtained desirable genotypes could be considered in new genetic selection schemes.

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Section: Discussionsupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Genetic polymorphism in melatonin receptor 1A and arylalkylamine N-acetyltransferase and its impact on seasonal reproduction in Egyptian sheep breeds

Fathy¹,

Gouda

Gafer³

et al. 2018

Arch. Anim. Breed.

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3 tesla magnetic resonance imaging and multiplanar reconstruction of the brain and its associated structures in sheep

Bakici

Ekim

Ergin

et al. 2019

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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The purpose of the study was to scan the brain and related structures in sheep with high-resolution magnetic resonance imaging (MRI) and three-dimensional (3D) multiplanar reconstruction for defining the anatomical regions. Six adult sheep, three of six were male and three of six were female used as cadaver, were utilized in this research. Heads were scanned at 3 Tesla Siemens Magnetom Spin-Echo MRI devices using the human head coil. The processes were acquired in both T1 and T2 weighted slices and were reconstructed by using Leonardo workstation. The cranioencephalic structures and anatomical details were defined and labeled in all slice of 3 planes that were obtained 1 mm thickness sequential images in sagittal, frontal, and transversal planes. According to the MRI measurements obtained intracranially from the brain, the average value of length, height, and width of the brain were 87.1 ± 0.3, 46.8 ± 0.7, and 62.2 ± 0.4 mm, respectively. It was observed that in T1 weighted images were more effective to identify deep brain structures and anatomical details. On 3D reconstructed images obtained from the study can be used as a reference in head and brain MR scans in multidisciplinary studies where sheep are used as animal models.

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Kıvırcık Irkı Koyunlarda Melatonin Reseptör 1A (MTNR1A) Geninin MnlI ve RsaI Restriksiyon Enzimleri ile Genetik Varyasyonunun Belirlenmesi

Cited by 2 publications

References 21 publications

Genetic polymorphism in melatonin receptor 1A and arylalkylamine N-acetyltransferase and its impact on seasonal reproduction in Egyptian sheep breeds

Genetic polymorphism in melatonin receptor 1A and arylalkylamine N-acetyltransferase and its impact on seasonal reproduction in Egyptian sheep breeds

3 tesla magnetic resonance imaging and multiplanar reconstruction of the brain and its associated structures in sheep

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