3 tesla magnetic resonance imaging and multiplanar reconstruction of the brain and its associated structures in sheep

Abstract

The purpose of the study was to scan the brain and related structures in sheep with high-resolution magnetic resonance imaging (MRI) and three-dimensional (3D) multiplanar reconstruction for defining the anatomical regions. Six adult sheep, three of six were male and three of six were female used as cadaver, were utilized in this research. Heads were scanned at 3 Tesla Siemens Magnetom Spin-Echo MRI devices using the human head coil. The processes were acquired in both T1 and T2 weighted slices and were reconstructed by using Leonardo workstation. The cranioencephalic structures and anatomical details were defined and labeled in all slice of 3 planes that were obtained 1 mm thickness sequential images in sagittal, frontal, and transversal planes. According to the MRI measurements obtained intracranially from the brain, the average value of length, height, and width of the brain were 87.1 ± 0.3, 46.8 ± 0.7, and 62.2 ± 0.4 mm, respectively. It was observed that in T1 weighted images were more effective to identify deep brain structures and anatomical details. On 3D reconstructed images obtained from the study can be used as a reference in head and brain MR scans in multidisciplinary studies where sheep are used as animal models.

An anatomical and volumetric study on brain ventricles in sheep using different techniques

“…The mean brain sizes obtained in our MR measurements were 83 ± 3.1, 52.5 ± 2.5 and 59.6 ± 6.2 mm. These MRI measurement results of the Akkaraman sheep were obtained by Bakıcı et al (2019) according to their data, it is higher. The size of the freshly extirpated brains of Akkaraman sheep is 130.78 × 40.58 × 70.08 mm on average.…”

“…The brain length, height and width values obtained by Bakıcı et al (2019) with MR imaging in sheep were 87.1, 46.8 and 62.2 mm, respectively. The mean brain sizes obtained in our MR measurements were 83 ± 3.1, 52.5 ± 2.5 and 59.6 ± 6.2 mm.…”

“…Thus, cerebrospinal fluid (CSF) loss from cranial and spinal subarachnoidal compartments was minimized. Sheep heads placed in a human head coil in the prone position on the mandible were imaged with a 1.5 Tesla MR scanner (Magnetom Essenza, Siemens Healthineers) in accordance with previous MR research procedures (Bakıcı et al, 2019;La Rosa et al, 2023;Schmidt et al, 2009Schmidt et al, , 2012.…”