Kinetics model for growth of Pseudomonas putida F1 during benzene, toluene and phenol biodegradation

Abuhamed, Tarık; Bayraktar, Emine; Mehmetoğlu, Tanju; Mehmetoǧlu, Ülkü

doi:10.1016/s0032-9592(03)00210-3

Cited by 176 publications

(102 citation statements)

References 17 publications

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“…The growth rate constant, k is in the range of 0.08 to 0.37 hr -1 , which was a measure of how fast the cells are dividing in a culture for the first 2 to 6 hours from the 30 hours experiments. The result obtained agreed with the previous findings where P. putida was found to overwhelmingly grow in a recalcitrant medium containing toxic organic wastes [11]. Figure 2 , presented the finding for P. putida (ATCC 49128) cell dried weight (gl 1-).…”

Section: Determination Of P Putida Growth Parameterssupporting

confidence: 91%

Effect of Acclimatization Time to Microbial Cell Growth and Biosynthesis of Mesophilic Gammaproteobacterium, in Orbital Shake Flasks

et al. 2017

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Abstract. Growth pattern of Pseudomonas putida (ATCC 49128), was found to predominantly rely on the age of the inoculums, prior to its contact with physical and chemical agents and nutrient availability. Under suitable inoculums, bacteria tend to grow faster in a batch type of growth pattern which is usually sustained until after nutrient depletion. In this research, the bacterial growth pattern was studied in an incubator shake flask using 8 g nutrient media and physical operational parameters temperature of 37 o C and agitation of 180 rpm over a period of 24, 48 and 72 hours. Prior to this, P. putida was added into 20.0 ml nutrient broth and incubated in an incubator for 24 hours at 37 o C, before adding it to 180 ml nutrient broth 30% (v/v 1-). Growth, via acclimatization was initially observed after 1hr of inoculation with an overwhelming exponential growth of 2.69-2.57 within first 24 hr, exceeding the 48 and 72 hrs ranges. This additionally relates to particular cell biomass growth rate (µ) of 0.58 hr 1-, 3.87 number of generation (n), generation time (g) 1.09 and growth rate constant (k) of 0.01 hr 1-, achievable within 24 hrs. It was therefore concluded that the sensitivity of this strain to time is significant, as optimal growth was achieved within 24 hrs of acclimatization, thereby showing a drastic reduction in the time of growth.

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Section: Determination Of P Putida Growth Parameterssupporting

confidence: 91%

Effect of Acclimatization Time to Microbial Cell Growth and Biosynthesis of Mesophilic Gammaproteobacterium, in Orbital Shake Flasks

et al. 2017

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“…Figure 10 shows the estimated (based on known toluene partition coefficient) aqueous phase toluene concentrations over the course of the 200-min experiment. The concentration of toluene at which inhibition occurs (i.e., specific growth rate declines) depends on the organism(s) employed and has been reported as 30 mg/L (Abuhamed et al, 2004), 43 mg/L (Reardon et al, 2000), and 70 mg/L (Elmen et al, 1997). As can be seen, with no second phase present, the aqueous phase toluene concentration remained high (>150 mg/L) throughout the experiment, while the polymer beads as a second phase case surpassed 100 mg/L approximately 20 min into the 60-min step, and the solvent as a second phase system never exceeded 20 mg/L of toluene throughout the step, however it also increased throughout the 60 min transient.…”

Section: Large Stepsmentioning

confidence: 99%

Transient performance of two‐phase partitioning bioreactors treating a toluene contaminated gas stream

Boudreau

Daugulis

2006

Biotech & Bioengineering

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Two-phase partitioning bioreactors (TPPBs) consist of a cell-containing aqueous phase and an immiscible organic phase that sequesters and delivers toxic substrates to cells based on equilibrium partitioning. The immiscible organic phase, which acts as a buffer for inhibitory substrate loadings, makes it possible for TPPBs to handle high volatile organic compound (VOC) loadings, and in this study the performance of liquid n-hexadecane and solid styrene butadiene (SB) polymer beads used as partitioning phases were compared to a single aqueous phase system while treating transient loadings of a toluene contaminated air stream by Achromobacter xylosoxidans Y234. The TPPBs operated as well-mixed stirred tanks, with total working volumes of 3 L (3 L aqueous for the single-phase system, 2 L aqueous and 1 L n-hexadecane for the solvent system, and 2.518 L aqueous volume and 500 g of SB beads for the polymer system). Two 60-min step changes (7 and 17 times the nominal loading rates, termed "small" and "large" steps, respectively) were imposed on the systems and the performance was characterized by the overall removal efficiencies, instantaneous removal efficiency recovery times (above 95% removal), and dissolved oxygen recovery times. For the small steps, with a nominal loading of 343 g/m3/h increasing to 2,400 g/m3/h, the TPPB system using n-hexadecane as the second phase performed best, removing 97% of the toluene fed to the system compared with 90% for the polymer beads system and only 69% for the single-phase system. The imposed large transient gave similar results, although the impact of the presence of a second sequestering phase was more pronounced, with the n-hexadecane system maintaining much reduced aqueous toluene concentrations leading to significantly improved performance. This investigation also showed that the presence of both n-hexadecane and SB beads improved the oxygen transfer within the systems.

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“…It is well known that many groundwater contaminants that can be degraded by soil-inhabiting bacteria at low concentrations via metabolism or cometabolism are toxic to them above certain threshold concentrations [6,7,19]. Prolonged exposure to higher concentrations of these contaminants may cause significant stress to bacterial cells that may result in temporary or permanent loss of their ability to degrade specific contaminants [20] or even in complete loss of cell viability.…”

Section: Introductionmentioning

confidence: 99%

Kinetics of trichloroethylene and toluene toxicity to Pseudomonas putida F1

Singh

Olson

2009

Enviro Toxic and Chemistry

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The goal of the present study was to elucidate the distribution of viable bacteria in chemical gradients and to evaluate the toxic effect of high concentrations of contaminants on contaminant-degrading bacteria under prolonged exposure. Accumulations of viable Pseudomonas putida F1 (P. putida F1) cells were observed surrounding trichloroethylene (TCE)-containing plugs. Results from this work indicate that P. putida F1 immediately adjacent to a TCE source become nonviable, whereas cells accumulating farther away use chemotaxis to migrate toward regions with optimal chemical concentrations in the form of concentrated bacterial bands. A method was developed to test the toxicity of model contaminant stressors, TCE and toluene, to P. putida F1; data obtained from toxicity experiments were fit to linear and exponential bacterial viability-decay models. Toxicity of TCE to P. putida F1 was best described with an exponential viability-decay model, with a viability-decay constant k(TCE) = 0.025 h(-4.95) (r(2) = 0.965). Toluene toxicity showed a marginally better fit to the linear viability-decay model (r(2) = 0.976), with a viability-decay constant k(toluene) = 0.208 h(-1). Best-fit model parameters obtained for both TCE and toluene were used to predict bacterial viability in toxicity experiments with higher contaminant concentrations and matched well with experimental data. Results from the present study can be used to predict bacterial accumulation and viability near nonaqueous-phase liquid (NAPL) sources in groundwater and may be helpful in designing bioremediation strategies for sites contaminated with residual NAPLs.

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Kinetics model for growth of Pseudomonas putida F1 during benzene, toluene and phenol biodegradation

Cited by 176 publications

References 17 publications

Effect of Acclimatization Time to Microbial Cell Growth and Biosynthesis of Mesophilic Gammaproteobacterium, in Orbital Shake Flasks

Effect of Acclimatization Time to Microbial Cell Growth and Biosynthesis of Mesophilic Gammaproteobacterium, in Orbital Shake Flasks

Transient performance of two‐phase partitioning bioreactors treating a toluene contaminated gas stream

Kinetics of trichloroethylene and toluene toxicity to Pseudomonas putida F1

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