Kineosporia babensis sp. nov., isolated from plant litter in Vietnam

Sakiyama, Yayoi; Thao, Nguyen Kim Nu; Giang, Nguyen Minh; Miyadoh, Shinji; Hop, Duong Van; Ando, Katsuhiko

doi:10.1099/ijs.0.002907-0

Cited by 17 publications

(14 citation statements)

References 26 publications

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“…Cell-wall amino acids were analyzed by using the method described by Tamura et al, 13 and the amino acid isomers in cell-wall peptidoglycan were analyzed using the method described by Nozawa et al 21 Biomass for chemotaxonomic studies was obtained by cultivation in shaken flasks containing YG broth (10 g glucose, 10 g yeast extract and 15 g l À1 agar; pH 7.3) for 24-72 h at 28 1C. 22 …”

Section: Chemotaxonomic Analysismentioning

confidence: 99%

Flexivirga alba gen. nov., sp. nov., an actinobacterial taxon in the family Dermacoccaceae

et al. 2011

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Section: Chemotaxonomic Analysismentioning

confidence: 99%

Flexivirga alba gen. nov., sp. nov., an actinobacterial taxon in the family Dermacoccaceae

et al. 2011

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“…Using OS Microbiol Indones 168 WIDYASTUTI ET AL. isolated from plant litter in Vietnam (Sakiyama et al 2009). The position of isolates of the family Kineosporiaceae selected in this study represented a line of descent distinct from previously described species of this family and further information are needed to describe the isolates (Fig 1).…”

Section: Discussionmentioning

confidence: 99%

Genus Diversity of Actinomycetes in Cibinong Science Center, West Java, Indonesia

et al. 2012

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Actinomycetes are microorganisms that play important role to support human health and known as soil microorganisms. The aim of the research was to describe genus diversity of actinomycetes in Cibinong Science Center (CSC), West Java. Samples for isolation were soil and plant litters. The samples were air dried and ground. We employed isolation methods: dry heat (DH), sodium dodecyl sulphates-yeast extract (SDS-YE), rehydration and centrifugation (RC), and oil separation (OS). A total of 263 isolates of actinomycetes were isolated in CSC, in 2004-2006. Totally 58, 144, 50, and 11 isolates were isolated under each isolation methods, respectively. All isolates were identified using the 16S rRNA gene sequencing method. The results showed that the isolates were belonged to the family Kineosporiaceae, Micromonosporaceae, Nocardiaceae, Pseudonocardiaceae, Streptomycetaceae, Streptosporangiaceae, Mycobacteriaceae, Nocardioidaceae, Nocardiopsaceae, and Thermomonosporaceae. There were 23 genera under those families. Homology value of the isolates based on BLAST search using 16S rRNA gene sequence data as queries showed that 136, 91, 30, and 6 isolates were ≥99, 98, 97, and ≤96%, respectively, compared to the known sequence in data base. The later 6 isolates were interesting for further identification leading to new taxa. Recognized species of Streptomyces genera under the member of the Streptomycetaceae were dominant among other isolates

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“…T was extracted and amplified by using the procedures described by Sakiyama et al (2009). The 16S rRNA gene sequence of strain VN05A0561…”

Section: Strain Vn05a0561mentioning

confidence: 99%

“…The phospholipid was of type PIII, and the polar lipid profile consisted of phosphatidyl-N-methylethanolamine, phosphatidylcholine, diphosphatidylglycerol, phosphatidylinositol and three unknown glycolipids. The chemotaxonomic properties of strain VN05A0561 T were consistent with the characteristics described for the genus Pseudonocardia.For 16S rRNA gene sequence analysis, the DNA of strain VN05A0561T was extracted and amplified by using the procedures described by Sakiyama et al (2009). The 16S rRNA gene sequence of strain VN05A0561…”

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confidence: 99%

Pseudonocardia babensis sp. nov., isolated from plant litter

Sakiyama

Thao

Vinh

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

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A novel actinomycete, designated strain VN05A0561T , was isolated from plant litter collected at Ba Be National Park, Vietnam. The substrate mycelia and spore chains fragmented in a manner similar to nocardioform actinomycetes; the spores had smooth surfaces and were rodshaped. Strain VN05A0561 T had the following chemotaxonomic characteristics: mesodiaminopimelic acid in the cell-wall peptidoglycan, arabinose and galactose as characteristic sugars, MK-8(H 4 ) as the major isoprenoid quinone, phosphatidylcholine as the diagnostic phospholipid and iso-C 16 : 0 as the major cellular fatty acid. Strain VN05A0561 T shared low levels of 16S rRNA gene sequence similarity (,97 %) with the type strains of recognized species of the genus Pseudonocardia and could be differentiated from its closest phylogenetic relatives based on phenotypic characteristics. These results suggested that strain VN05A0561 T represents a novel species of the genus Pseudonocardia, for which the name Pseudonocardia babensis sp. nov. is proposed. The type strain is VN05A0561 T (5VTCC-A-The genus Pseudonocardia was established as a nocardioform actinomycete by Henssen (1957), and its description has been emended based on both chemotaxonomic and morphological variations (Reichert et al., 1998;Huang et al., 2002;Park et al., 2008). On the basis of the lack of mycolic acids and the presence of cell-wall type IV, the genera Amycolata (Lechevalier et al., 1986) and Pseudoamycolata were also proposed as nocardioform actinomycetes. However, the latter two genera were found to have chemotaxonomic properties similar to those of the genus Pseudonocardia (Kothe et al., 1989;Takeuchi et al., 1992), and were incorporated in the genus Pseudonocardia on the basis of 16S rRNA gene sequence analysis (Warwick et al., 1994;McVeigh et al., 1994). Subsequently, members of the genus Actinobispora (Jiang et al., 1991) were also transferred to the genus Pseudonocardia based on identification with the use of specific PCR primers, and re-analysis of 16S rRNA gene sequences and menaquinones of the type species (Huang et al., 2002 Reichert et al., 1998;Gu et al., 2006; Chen et al., 2009).In the present study, a Pseudonocardia-like strain isolated from a plant litter sample collected during the course of a study on the diversity of actinomycetes inhabiting Vietnam (Sakiyama et al., 2009) was studied by using a polyphasic taxonomic approach.Plant litter samples were collected at the Ba Be National Park, Bac Kan, in the mountainous area of northern Vietnam. Samples were dried at room temperature for 5-7 days and subsequently used for isolation. Rehydration and centrifugation methods (Hayakawa et al., 2000) were employed for isolation by using humic acid-vitamin agar (Hayakawa & Nonomura, 1987) containing nalidixic acid (20 mg l 21) and kabicidine (0.75 mg l 21). Strain VN05A0561 T was isolated after incubation for more than 10 days at room temperature. Strain VN05A0561T was cultured on yeast extract-soluble starch medium (YS medium; per litre distilled water: 2 g yeast extract...

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Kineosporia babensis sp. nov., isolated from plant litter in Vietnam

Cited by 17 publications

References 26 publications

Flexivirga alba gen. nov., sp. nov., an actinobacterial taxon in the family Dermacoccaceae

Flexivirga alba gen. nov., sp. nov., an actinobacterial taxon in the family Dermacoccaceae

Genus Diversity of Actinomycetes in Cibinong Science Center, West Java, Indonesia

Pseudonocardia babensis sp. nov., isolated from plant litter

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