Genus Diversity of Actinomycetes in Cibinong Science Center, West Java, Indonesia

Widyastuti, Yantyati; Lisdiyanti, Puspita; Ratnakomala, Shanti; Kartina, Gina; Ridwan, Roni; Rohmatussolihat, Rohmatussolihat; Prayitno, Nita Rosalinda; Triana, Evi; Widhyastuti, Nunuk; Saraswati, Rasti; Hastuti, Ratih Dewi; Lestari, Yulin; Otoguro, Misa; Miyadoh, Shinji; Yamamura, Hideki; Tamura, Tomohiko; Ando, Katsuhiko

doi:10.5454/mi.6.4.4

Cited by 5 publications

(4 citation statements)

References 29 publications

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“…One of the groups of bacteria that produce many important antibiotics is Actinobacteria. Actinobacteria are Gram-positive, have high GC content, and comprise various genera known for their secondary metabolite production, such as Streptomyces , Micromonospora , Kitasatospora , Nocardiopsis , Pseudonocardia , Nocardia , Actinoplanes , Saccharopolyspora , and Amycolatopsis [16,17]. Their most important genus is Streptomyces , which has a filamentous form like fungi and has become a source of around two-thirds of all known natural antibiotics [18].…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis of Polyketides in Streptomyces

2019

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Polyketides are a large group of secondary metabolites that have notable variety in their structure and function. Polyketides exhibit a wide range of bioactivities such as antibacterial, antifungal, anticancer, antiviral, immune-suppressing, anti-cholesterol, and anti-inflammatory activity. Naturally, they are found in bacteria, fungi, plants, protists, insects, mollusks, and sponges. Streptomyces is a genus of Gram-positive bacteria that has a filamentous form like fungi. This genus is best known as one of the polyketides producers. Some examples of polyketides produced by Streptomyces are rapamycin, oleandomycin, actinorhodin, daunorubicin, and caprazamycin. Biosynthesis of polyketides involves a group of enzyme activities called polyketide synthases (PKSs). There are three types of PKSs (type I, type II, and type III) in Streptomyces responsible for producing polyketides. This paper focuses on the biosynthesis of polyketides in Streptomyces with three structurally-different types of PKSs.

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Section: Introductionmentioning

confidence: 99%

Biosynthesis of Polyketides in Streptomyces

2019

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“…J. Microbiol., 10 (12): 582-591, 2015 broth medium using DNA extraction kit (Promega, USA) (Widyastuti et al, 2012). The 16S rRNA PCR then preformed using the 5'-GAGTTTGATCCTGGCTCAG-3' forward primer and 5'-AAGGAGGTGATCCAGCC-3' reverse primer.…”

Section: Methodsmentioning

confidence: 99%

In Silico Analysis of Plantaricin EF that Expressed by Plasmid-Associated Bacteriocin Production Gene of Lactobacillus plantarum IBL-2 for Anti-Candida Agent Potential

Nurhayati¹,

Wibowo²,

Widyastuti³

et al. 2015

Research J. of Microbiology

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Lactobacillus plantarum species often harbor several plasmids. These plasmids may encoded important traits such as phages or antibiotics resistances, lactose catabolism and production of proteolytic enzyme and also bacteriocins that named plantaricin. Lactobacillus plantarum IBL-2 that isolated from strawberry of Bali plantation have the highest anti-microorganism activity among the L. plantarum isolate collection of BTCC Indonesia. Only few study carried out to examine the antifungal activity of bacteriocin from L. plantarum. This study focus on anti-Candida activity of plasmid associated with bacteriocin production from L. plantarum IBL-2. The isolates were confirmed by the 16S rRNA analysis by PCR and the phylogenetic tree was built based on references sequences and one outgroups from database. The plantaricin gene screening then performed in plasmid that was isolated from L. plantarum IBL-2 by PCR using five pairs or plantaricin gene primer. The anti-Candida potential of plantaricin observed were analyzed by in silico by docking analysis between the plantaricin and receptors of apoptosis proteins. PlnB and PlnEF were observed in the L. plantarum plasmid. Only PlnEF become the focus of study. Analysis of docking study predicted that PlnEF have interactions with apoptosis proteins regulator in eukaryotic cells. PlnEF that encoded by plasmid of L. plantarum may exert anti-Candida potential through interactions with apoptosis proteins regulator.

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“…Actinomycetes are found abundantly in the environment and have a large diversity of genera (Lisdiyanti et al 2012;Widyastuti et al 2012;Ratnakomala et al 2016;Sulistyani et al 2014). One of the actinomycetes habitats is the marine environment.…”

Section: Introductionmentioning

confidence: 99%

The potency of actinomycetes extracts isolated from Pramuka Island, Jakarta, Indonesia as antimicrobial agents

et al. 2021

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Abstract. Setiawati S, Nuryastuti T, Sholikhak EN, Lisdiyanti P, Pratiwi SUT, Sulistiyanti TR, Ratnakomala S, Jumina, Mustofa. 2021. The potency of actinomycetes extracts isolated from Pramuka Island, Jakarta, Indonesia as antimicrobial agents. Biodiversitas 22: 1104-1111. Actinomycetes are one of the Gram-positive bacteria which are widely distributed and produce many secondary metabolites including those known as antibiotics, antifungals, anticancer, and antimalarials agents. The secondary metabolites of actinomycetes are abundant, which include many active compounds that have been identified because of the large diversity in the actinomycetes phylum. This study aimed to identify and screen collected by Indonesian Culture Collection (InaCC) from Bojong Gede and Pramuka Island, Jakarta, Indonesia as antibacterial and antifungal agents. Primary screening was done on 16 actinomycetes isolates by well-agar diffusion method. Antimicrobial activity was tested by using micro broth dilution methods to determine minimum inhibitory concentration (MIC). Molecular identification into level genera and species was determined by 16S rRNA gene sequencing. Out of 16 actinomycetes isolates used, 4 isolates have activity against Candida albicans ATCC 10231, Staphylococcus aureus ATCC 6538, Bacillus subtilis BTCC B-612, and Escherichia coli BTCC B-614, specifically InaCC A758, InaCC A759, InaCC A760 and InaCC A765 isolates. InaCC A758 have highest antimicrobial activity against mentioned microbial with MIC value at 50 μg/mL, 6.25 μg/mL, 31.25 μg/mL and 3.125 μg/mL, respectively. Three genera were found from the samples: i.e. Streptomyces (80%), Microbispora (13%) and Nocardia (6%). Based on 16S rRNA gene identification, the active isolates of actinomycetes InaCC A758, InaCC A759, InaCC A760 and InaCC A765 were similar to Streptomyces badius, Streptomyces olivaceus, Streptomyces sanyensis, and Nocardia otitidiscaviarum, respectively. The secondary metabolites of actinomycetes extracts from Pramuka Island can be potentially developed as antifungal and antibacterial agents.

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Genus Diversity of Actinomycetes in Cibinong Science Center, West Java, Indonesia

Cited by 5 publications

References 29 publications

Biosynthesis of Polyketides in Streptomyces

Biosynthesis of Polyketides in Streptomyces

In Silico Analysis of Plantaricin EF that Expressed by Plasmid-Associated Bacteriocin Production Gene of Lactobacillus plantarum IBL-2 for Anti-Candida Agent Potential

The potency of actinomycetes extracts isolated from Pramuka Island, Jakarta, Indonesia as antimicrobial agents

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