Kidney histology in streptozotocin-induced diabetic male Wistar rats treated with combined extract of temulawak rhizome and belimbing wuluh fruit

Abstract

Alipin K, Sari EP, Madihah, Setiawati T, Ratningsih N, Malini DM. 2017. Kidney histology in streptozotocin-induced diabetic male Wistar rats treated with combined extract of temulawak rhizome and belimbing wuluh fruit. Nusantara Bioscience 9: 312-317. Complications that are occurred in patients with Diabetes Mellitus usually followed by kidney damage. Temulawak (Curcuma xanthorrhiza Roxb.) and belimbing wuluh (Averrhoa bilimbi L.) were traditionally used to decrease blood glucose level. Thus, they were potential as antidiabetic drugs. This study aimed to evaluate the combination of ethanol extracts of temulawak rhizome and belimbing wuluh fruit in repairing kidney damage in diabetic male Wistar rats induced by streptozotocin (STZ). An experimental method using a completely randomized design that consist of seven treatments with three replications. Six treatment groups were injected intraperitoneally with a dose of 60 mg/kg BW STZ, and one group served as a control. The animals which have blood glucose level ≥200 mg/dl were stated as diabetic. Furthermore, the animals were treated orally with single extract i.e. temulawak 17.5 mg/kg BW or belimbing wuluh 750 mg/kg BW and combined extracts 383.75 or 767.5 mg/kg BW, as well as glibenclamide 0.45 mg/kg BW as reference, including diabetic rat as positive control and non-diabetic rat as negative control. The results showed that combine extract at dose of 383.75 mg/kg BW treatment repaired the kidney histology, i.e., glomerular diameter and Bowman space width, as well as significantly decreased the necrosis percentage of proximal tubular in diabetic rat compared with positive control group (p<0.05). In conclusion, the combined extract of temulawak rhizome and belimbing wuluh fruit has potent to cure renal failure in diabetic rats induced by streptozotocin.

Effect of Asparagus africanus (Asparaceae) Aqueous Extract on Renal Complications in High Fat Diet and Streptozotocin-Induced Type 2 Diabetes in Male albino Wistar Rats

“…The histopathological examination result in this study agrees with Zafar, et al [35] and Alipin, et al [36] who found that the relative kidney weight in STZinduced rat for 12 weeks was significantly higher than that of the non-induced rat. In this study, the Bowman's space in kidneys of diabetic rats was wider than of nondiabetic rats.…”

The Role of Black Soybean and Purple Sweet Potato Active Compound on Advanced Glycation End-Product in Streptozotocin-Induced Type 2 Diabetes Mellitus Rat

“…ROS stimulate the overexpression of pro-sclerotic growth factors such as tumor growth factor β (TGFβ) and connective tissue growth factor (CTGF). In line with our finding, the percentage of necrosis on the proximal tubule of DM rats was higher than nondiabetes rats [25]. Damage on tubules characterizes pyknosis, karyolysis, and necrosis on the brush border.…”

Watermelon (Citrullus lanatus) leaf extract attenuates biochemical and histological parameters in high‐fat diet/streptozotocin‐induced diabetic rats

“…To further investigate the protective effects of watermelon leaf extract against diabetes, we examined the liver and kidney structures of the experimental animals. These organs are primarily involved in glucose metabolism (Alipin et al, 2017). The kidneys of the diabetic rats showed renal tubular necrosis and hyaline cast formation in the necrotic regions (Chowdhury et al, 2019).…”