Karakterisasi Mutu Ekstrak Daun Sirsak (Annona muricata L.) dari Tiga Tempat Tumbuh

Setyorini, Herni Asih; Kurniatri, Arifayu Addiena; Adelina, Rosa; Adelina, Adelina

doi:10.22435/bpk.v44i4.5184.279-286

Cited by 9 publications

(5 citation statements)

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“…Tannins, steroids, and terpenoids also found in soursop leaves; however, this study showed negative results of terpenoids and positive effects of alkaloids and flavonoids as the main compounds in A.muricata. The results of this study were not too different from the other research by Setyorini et al phytochemical screening results in the study showed that A. muricata leaves ethanolic extract contained alkaloid, flavonoid, tannin, and steroid (Setyorini et al, 2016).…”

Section: Resultscontrasting

confidence: 85%

The Cytotoxic Activity of Annona muricata Linn Leaves Ethanolic Extract (AMEE) on T47D Breast Cancer Cell Line

2020

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Breast cancer is the most common cancer in women throughout the world, with new cases and deaths which continue to increase. Soursop leaves (Annona muricata L) have been used extensively in traditional medicine, including cancer. Acetogenin, alkaloids, and phenols contained in soursop leaves are known to have anti-cancer effects. Among them, acetogenin has the most dominant role and reported to have a cytotoxic effect on various cancer cell lines. This study aims to determine the cytotoxic activity of soursop leaf ethanol extract on T47D breast cancer cell line. Measurement of cytotoxic activity was carried out by the MTT method, and the viability percentage of T47D cells was calculated based on the absorbance values in the treatment, cell control, and media control groups of each replicate. The correlation between extract concentration and viability percentage of the T47D cell line was outlined in the regression equation to obtain the IC50 value. IC50 values of 109.91 ± 3.04 with R values 0.975 and R2 0.9508 obtained. R values close to 1 indicated a strong correlation between extract concentration and the percentage of living T47D cells. Meanwhile, the amount of R2 suggested that the level of AMEE had a 95.08% influence on the rate of cell viability, and the other 4.92% influenced by factors other than the AMEE dose. These results indicated that the ethanol extract of soursop leaves has a cytotoxic effect and has the potential to inhibit T47D cell proliferation in vitro.

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Section: Resultscontrasting

confidence: 85%

The Cytotoxic Activity of Annona muricata Linn Leaves Ethanolic Extract (AMEE) on T47D Breast Cancer Cell Line

2020

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“…High levels of water cause low shelf life of extracts because they are prone to fungi (Setyorini et al, 2016). One of them is Aspergillus flavus which can produce aflatoxins which are harmful to health (Ratnani et al, 2017).…”

Section: Resultsmentioning

confidence: 99%

Simplicia and Extracts Standardization from Jualing Leaves (Micromelum minutum Wight & Arn.) from South Kalimantan

Sutomo

2019

Borneo J Pharm

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Jualing (Micromelum minutum Wight & Arn.) is a plant from South Kalimantan that has the potential to be developed as natural medicine. This study aims to establish standardization which includes specific and non-specific parameters of M. minutum leaf simplicia and extracts from three growing locations, namely Banua Botanical Garden, Sultan Adam Forest Park, and Forest Areas with Specific Objects of Rantau, South Kalimantan. The method of setting standardization parameters refers to the Indonesian Herbal Pharmacopoeia and General Standard Extracts Parameters. Organoleptic standardization of simplicia is brownish-green, has a distinctive odor, has a bitter and slightly spicy taste. Microscopic observations showed the presence of stomata, cell walls, cytoplasm, calcium oxalate crystals, upper epidermis, palisade tissue, spongy tissue, cortex, xylem, phloem, lower epidermis, and trichomes. Water content test showed the results of 21.9-22.07%; ethanol extract content of 12.87-13.17%; drying losses 4.64-4.84%; total ash content of 6.04-6.14%; acid insoluble ash content 1.13-1.19%; Pb levels of 0.022-0.025 mg/kg; Cd levels of 0.017-0.020 mg/kg; and Hg levels <0.0004 mg/kg. Extract standardization shows thick green-black, thick-smelling extract and bitter taste. Micromelum minutum leaves contain alkaloids, flavonoids, saponins, terpenoids, tannins, anthraquinones, phenols, and glycosides. Thin Layer Chromatography profiles show the similarity of chemical compounds in each growth location. Other parameters are extract yield of 8.08-8.32%; moisture content of 6.07-6.27%; total ash content of 4.22-4.27%; and acid insoluble ash content 1.11-1.12%. All standardization parameters meet the requirements in the Media Materia Indonesia and the Republic of Indonesia Drug and Food Control Center.

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“…The environment is a factor that is very instrumental in the biosynthesis of metabolites in plants. Plants can regulate the production of metabolites in accordance with the changing factors that exist in the environment, especially light, which is very instrumental in the biosynthesis of its compounds (Setyorini et al 2016). In addition, things that can affect the levels of a compound in plants include the geographical location of the plant and climatic factors, including temperature, air, and humidity.…”

Section: Discussionmentioning

confidence: 99%

Toxicity Activity and Total Phenolic Content of Soursop Leaves from Three Regions in South Sulawesi, Indonesia

Rasyid,

Amin,

Sukmawati

et al. 2023

JECP

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The fruit plant known as the soursop (Annona muricata L.) is indigenous to the Caribbean, Central America, and South America. The leaves of soursop contain substances with the potential to be cytotoxic, such as phenols and anonaceous acetogenins. Numerous variables, including geographic location, climatic conditions, and pest or disease disturbance factors, might impact a compound's phenol levels and toxicity. This study aims to investigate whether there are variations in the phenolic content and toxicity of the ethanol extract of soursop leaves that are grown in Gowa, Takalar, and Pinrang, three distinct regions in South Sulawesi, Indonesia. The Folin-Ciocalteu reagent was used to evaluate the total phenolic content, whereas Artemia salina Leach larvae were used for the BSLT method of the toxicity test. The results showed that the extracts of soursop leaves from Gowa, Pinrang, and Takalar had total phenolic contents of 3.7588% mg GAE/g, 0.9686% mg GAE/g, and 1.3832% mg GAE/g, respectively. Gowa, Pinrang, and Takalar have LC50 values of 38.19 µg/mL, 34.35 µg/mL, and 70.95 µg/mL, respectively, indicating their toxicity. The conclusion is that there were differences in the phenolic content and toxicity level of the ethanol extract of soursop leaves. All of these areas could source raw materials of soursop for the development of herbal medicines with cytotoxicity, i.e., anticancer, because they have LC50< 1,000 µg/mL.

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Karakterisasi Mutu Ekstrak Daun Sirsak (Annona muricata L.) dari Tiga Tempat Tumbuh

Cited by 9 publications

References 0 publications

The Cytotoxic Activity of Annona muricata Linn Leaves Ethanolic Extract (AMEE) on T47D Breast Cancer Cell Line

The Cytotoxic Activity of Annona muricata Linn Leaves Ethanolic Extract (AMEE) on T47D Breast Cancer Cell Line

Simplicia and Extracts Standardization from Jualing Leaves (Micromelum minutum Wight & Arn.) from South Kalimantan

Toxicity Activity and Total Phenolic Content of Soursop Leaves from Three Regions in South Sulawesi, Indonesia

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