ISSR-PCR fingerprinting of Ukrainian sweet cherry (Prunus avium L.) cultivars

Ivanovych, Ya. I.; Udovychenko, K.; Bublyk, M.O.; Волков, Р. А.

doi:10.3103/s0095452717010066

Cited by 14 publications

(6 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…With the minimum repeat length of 12 base-pairs they are tandemly repeated usually 5-20 times in the genome (Goodfellow 1992;Vaughan and Lloyd 2003;Ellegren 2004;Prajapati et al 2017). As a result of their quickness, simplicity, rich polymorphism and stability SSR markers are highly popular in genetic diversity analysis (Turkoglu et al 2013;Gürcan et al 2015;Batnini et al 2016), construction of fingerprints (Cantini et al 2001;Rojas et al 2008;Klabunde et al 2014;Turet-Sayar et al 2012;Ivanovych et al 2017), genetic purity test (Spann et al 2010), molecular map construction and gene mapping (Ogundiwin et al 2009;Olukolu et al 2009;Fan et al 2010;Pacheco et al 2014;Rowland et al 2014;Wang et al 2014;Eduardo et al 2015), utilization of heterosis, especially in the identification of species that are genetically related. Microsatellite markers have also been used in several studies to define conserved regions among related species (Decroocq et al 2003;Martínez-Gómez et al 2003;Maghuly and Laimer 2011;Alisoltani et al 2016) for both plants and animals genome mapping (Weising et al1998).…”

Section: Introductionmentioning

confidence: 99%

Preliminary results of SSR based characterization of sour (Prunus cerasus L.) and sweet cherry (Prunus avium L.) genotypes cultivated in Hungary

Eren¹,

Bedő²,

Edosa³

et al. 2017

Columella

View full text Add to dashboard Cite

Cherry cultivation in the Carpathian basin area began more than 100.000 years ago. Adapting to the basin specific ecological conditions resulted in high degree of genetic variability among the cherry cultivars. The SSR (Simple Sequence Repeat) markers allow the discrimination of the cultivars and determination their specific DNA fingerprints. Due to the high degree of polymorphism of microsatellite markers, generally only six SSR loci are enough to differentiate the varieties. Microsatellite markers are used not only for cultivar identification but also for the verification of synonyms and homonyms. Owing to their locus specificity and Mendelian codominant inheritance, parentage can be clearly identified, primary and secondary relationships between the cultivars can be discovered. The aim of this research was to characterize 29 sour cherry (Prunus cerasus L.), and 38 sweet cherry (Prunus avium L.) genotypes cultivated in Hungary to establish their DNA fingerprints in 6 SSR loci by allele numbers and sizes.

show abstract

Section: Introductionmentioning

confidence: 99%

Preliminary results of SSR based characterization of sour (Prunus cerasus L.) and sweet cherry (Prunus avium L.) genotypes cultivated in Hungary

Eren¹,

Bedő²,

Edosa³

et al. 2017

Columella

View full text Add to dashboard Cite

show abstract

“…Principal component analysis (PCA) showing the variance-covariance matrix from the marker data was performed using the MVSP v3.2 package. Parameters such as polymorphic information content (PIC), marker index (MI), resolving power (Rp) and effective multiplex Ratio (E) were calculated to estimate the suitability of ISSR primers for genetic profiling [22].…”

Section: Issr Analysismentioning

confidence: 99%

Genetic diversity and phylogenetic analysis of Robinia pseudoacacia L. populations using ISSR Markers, ITS1 and trnL-F Intergenic spacer sequences

Uras

Özyiğit

Filiz

et al. 2023

Preprint

View full text Add to dashboard Cite

Background Robinia pseudoacacia L. is a deciduous tree planted almost all around the world for a wide variety of uses such as ornamental in urban ecosystems and forest trees in afforestation. This study aimed to evaluate the genetic diversity of of R. pseudoacacia plants planted in urban ecosystems using R. pseudoacacia genotypes collected from Istanbul (Prince Island, Bağdat Avenue, Barbaros Boulevard and TEM highway) and Kocaeli (Dilovası District) cities. Methods and Results 15 ISSR primers were tested, nine of which gave clear and distinguishable bands with a total of 100 loci. The percentage of polymorphic loci (PPL) was calculated as 100% for multi-populations and ranged from 46% to 76% for single populations. Genetic diversity values based on Nei’s (1978) were calculated between 0.165 and 0.251. The lowest and highest PPL were found in Barbaros Boulevard and Dilovası District populations, respectively. Population structure analysis showed seven different structures for the five populations. The most genetically similar populations were found as Prince Island and Barbaros Boulevard populations, while the most diverged population was the Dilovası population. Phylogeny analysis was conducted based on ITS1 and trnL-F IGS sequences. The length of the ITS1 sequence was found as 239 bp, and the GC content ranged from 52.72% to 56.56% while the length of the trnL-F IGS sequence was found as 453 bp with GC content between 29.14-29.80%. Conclusion It is considered that the main reasons for the genetic similarities and differences is originating from either the parents of these trees or the pollution they are exposed to. In phylogenetic analysis, both region sequences showed a high discriminant value and effective discriminatory power at the family level.

show abstract

“…Genetic studies of the Cerasus species collections were carried out using various types of molecular markers. Using ISSR markers were study the species genetic structures: Prunus pseudocerasus [3; 4], Prunus cerasus [5], Prunus avium L. [6], Prunus mahaleb L., Prunus incana Pall., Prunus microcarpa Boiss. and Prunus brachypetala Boiss [7].…”

Section: Introductionmentioning

confidence: 99%

Identification of Sakura interspecific hybrids in Prunus collections

2021

View full text Add to dashboard Cite

The use of IRAP and ISSR markers for the genetic analysis of Cerasus and Padus samples from the NCFRCHVW collection made it possible to establish the collection genetic structure and identify interspecific hybrids of cherry trees. Clustering of genotyped samples revealed 4 main clusters: 1) Bird cherry; 2) Cherries; 3) Interspecific hybrids of sakura; 4) Sakura. Most of the hybrid forms of sakura and cherries have formed a separate group, which is different from both sour and sweet cherry varieties, and from the classic sakura varieties. Also, some samples were identified that were assigned to groups that were not typical for them. These samples include the genotype of the Sibirskaya krasavitsa bird cherry, AI72 rootstock, Podbelskaya cherry, Polskaya sakura and ornamental cherry Rexii. In general, ISSR and IRAP markers have demonstrated their effectiveness as tools for genetic analysis of Prunus collections and identification of genotypes arising in the course of interspecific hybridization.

show abstract

ISSR-PCR fingerprinting of Ukrainian sweet cherry (Prunus avium L.) cultivars

Cited by 14 publications

References 10 publications

Preliminary results of SSR based characterization of sour (Prunus cerasus L.) and sweet cherry (Prunus avium L.) genotypes cultivated in Hungary

Preliminary results of SSR based characterization of sour (Prunus cerasus L.) and sweet cherry (Prunus avium L.) genotypes cultivated in Hungary

Genetic diversity and phylogenetic analysis of Robinia pseudoacacia L. populations using ISSR Markers, ITS1 and trnL-F Intergenic spacer sequences

Identification of Sakura interspecific hybrids in Prunus collections

Contact Info

Product

Resources

About