Isolation, structure elucidation and biological activity of angucycline antibiotics from an epiphytic yew streptomycete

Abstract

In the course of study of epiphytic microorganisms occurring on the surface of roots of Taxus baccata L. a new strain Streptomyces sp. AC113 was isolated. According to 16S ribosomal DNA-based identification the new strain is 99% identical with Streptomyces flavidofuscus. This strain cultivated in an arginine glycerol medium produced three major metabolites identified as (-)-8-O -methyltetrangomycin (1), 8-O -methyltetrangulol (2) and 8-O -methyl-7-deoxo-7-hydroxytetrangomycin (3). The chemical structures of these angucyclines were elucidated by 1D and 2D NMR as well as by mass spectrometry. Isolated angucycline metabolites showed significant antimicrobial activity against Bacillus cereus and Listeria mocytogenes. Cytotoxic activities of compounds 1, 2 and 3 against four cell lines (B16, HT-29 and non - tumor V79, L929) were evaluated. Compound 3 was the most potent anticancer agents with IC(50) 0.054 microg/ml against cell line B16.

Adaptation mechanisms of bacteria during the degradation of polychlorinated biphenyls in the presence of natural and synthetic terpenes as potential degradation inducers

“…The sample was injected at a split ratio of 1/20 (injector 230°C; hydrogen flow 51 ml min −1 for 2 min, then 20 ml min −1 ; pressure 220 kPa). The fatty acid methylester peaks were identified by comparison to the authentic standards of C 4 -C 24 fatty acid methylesters mixture (Supelco, USA) and evaluated by ChemStation B 01 03 (Agilent Technologies, USA; Maruna et al 2010). The index of fatty acid unsaturation (IU) was calculated as Δ mol −1 according to the equation: IU0[1 (% monoenes)+2 (% dienes)]/100 (Čertík and Šajbidor 1996).…”

Bacterial cell membrane adaptation responses on stress caused with the environmental pollutants

“…The sample was injected at a split ratio of 1/20 (Injector 230 °C; hydrogen fl ow 51 ml.min -1 for 2 min, then 20 ml.min -1 ; pressure 220 kPa). The fatty acid methylester peaks were identifi ed by authentic standards of C 4 -C 24 fatty acid methylesters mixture (Supelco, USA) and evaluated by ChemStation B 01 03 (Agilent Technologies) (Maruna et al, 2010).…”

Antimicrobial Activity of Endophytes from Brazilian Medicinal Plants

“…All identified taxon in this study, Alternaria (Kjer et al, 2009), Phomopsis (Du et al, 2008, Penicillium (Bertinetti et al, 2009), Pestalotiopsis (Liu, 2011, basidiomycete (Suay et al, 2000) and Streptomyces (Maruna et al, 2010) had already been described as producers of metabolite with antimicrobial activity. Although the TLC method is only qualitative, it is a simple and relatively cheap technique to recognize the inhibitory activity of a large quantity of organic extracts.…”