Adaptation mechanisms of bacteria during the degradation of polychlorinated biphenyls in the presence of natural and synthetic terpenes as potential degradation inducers

Zorádová-Murínová, Slavomíra; Dudášová, Hana; Lukáčová, Lucia; Čertı́k, Milan; Šilharová, Katarína; Vrana, Branislav; Dercová, Katarı́na

doi:10.1007/s00253-011-3763-8

Cited by 18 publications

(17 citation statements)

References 43 publications

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“…This parameter achieved half value of control accumulation. These results are in agreement with those reported by Zorádová et al (2011) and Zorádová-Murínová et al (2012) where signifi cant reduction of growth of different bacterial strains was observed when persistent organic pollutants were added at the time of inoculation. Opposite effect was observed by Dercová et al (2004).…”

Section: Effect Of Pcbs and 3-cba On The Bacterial Growth And Lipid Asupporting

confidence: 83%

Bacterial cell membrane adaptation responses on stress caused with the environmental pollutants

Murínová¹,

Dercová²

2013

Acta Chimica Slovaca

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The effect of polychlorinated biphenyls on biomass production, lipid accumulation, and on the fatty acid profi le of the major membrane lipids of Alcaligenes xylosoxidans isolated from a soil long-term contaminated with polychlorinated biphenyls was examined. The lowest bacterial growth was observed in the presence of biphenyl and polychlorinated biphenyls. On the other hand, the highest growth stimulation was observed in the presence polychlorinated biphenyls. Higher growth ability was observed when polychlorinated biphenyls or 3-chlorobenzoic acid were added after three days of cultivation. Higher lipid accumulation corresponded with lower growth ability. The importance of time when bacterial strain meets polychlorinated biphenyls or 3-chlorobenzoic acid should be emphasized. The addition of toxic organic compound later in stationary growth phase led to better survival and evoked lower membrane adaptation. However, polychlorinated biphenyls or 3-chlorobenzoic acid amendment at the beginning of cultivation stimulated adaptation responses as membrane saturation, cis to trans isomerisation, and branched fatty acids alteration and decreased growth ability. Palmitic acid and 17-cyclopropyl fatty acid were observed as prevalent in the bacterial membrane as well as in the total lipids.

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Section: Effect Of Pcbs and 3-cba On The Bacterial Growth And Lipid Asupporting

confidence: 83%

Bacterial cell membrane adaptation responses on stress caused with the environmental pollutants

Murínová¹,

Dercová²

2013

Acta Chimica Slovaca

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“…Anteiso fatty (3-methyl) acids exert stronger influence on membrane fluidity than exerted by iso (2-methyl) isomers due to their steric configuration and different transition temperatures. Under toxic conditions, bacteria increase the production of iso fatty acids on the expense of anteiso forms to decrease the membrane fluidity (Unell et al 2007;Zorádová-Murínová et al 2012). The 3-CBA addition to the three-day-old cultures revealed enhancement of the adaptation mechanism compared to the addition of PCBs with all strains apart from A. xylosoxidans.…”

Section: Discussionmentioning

confidence: 96%

“…It is expressed as the lipid mass percentage in the dry biomass (Zorádová-Murínová et al 2012). In P. stutzeri, the lipid accumulation exceeded control value when PCBs (1.6 times over control) and 3-CBA (4 times over control) were added to the three-day-old culture ( Fig.…”

Section: Resultsmentioning

confidence: 99%

The adaptation responses of bacterial cytoplasmic membrane fluidity in the presence of environmental stress factors — polychlorinated biphenyls and 3-chlorobenzoic acid

et al. 2014

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Only bacteria sufficiently resistant to the toxic compounds in their environment can be used for the efficient biodegradation process in order to eliminate a widespread contamination by polychlorinated biphenyls (PCBs). The presence of PCBs results in bacterial controlled rigidification of cytoplasmic membrane. The four bacterial isolates from long-term PCB-contaminated soil (Alcaligenes xylosoxidans, Pseudomonas stutzeri) and sediment (Ochrobactrum anthropi, Pseudomonas veronii) have been used to select the strain most adapted to the PCBs, i.e. with efficient changes in the membrane phospholipid fatty acids. PCBs and their toxic degradation products -the 3-chlorobenzoic acids (3-CBA as the most toxic one) -were added separately to the liquid medium with glucose in two experimental sets: at lag phase and in stationary phase of bacterial growth in order to evaluate the effects of chemicals to cytoplasmic membrane. The main parameter -the changes in fatty acids composition (in the total lipids and the main membrane phospholipid phosphatidyletanolamine) were studied. 3-CBA caused growth inhibition when added at lag phase. However, when added during the stationary growth, inhibition was not observed. Similarly, after addition of PCBs to the stationary growth culture, inhibition of growth was not observed with all tested strains (except for P. stutzeri). This fact indicates the importance of time contact of bacteria during growth phase with xenobiotics. O. anthropi and A. xylosoxidans appeared to be the most adapted to the presence of PCBs (with sufficient membrane adaptation), active under the adverse conditions, and able to survive in the contaminated environment.

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“…The mechanisms of these compounds have not been described in detail yet. However, we described that some of these compounds are able to diminish bacterial adaptation mechanisms relating to fatty acids composition [138]. Natural matrices rich in terpene content belong to this group.…”

Section: Other Adaptation Responsesmentioning

confidence: 99%

Response Mechanisms of Bacterial Degraders to Environmental Contaminants on the Level of Cell Walls and Cytoplasmic Membrane

Murínová

Dercová

2014

International Journal of Microbiology

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152

112

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Bacterial strains living in the environment must cope with the toxic compounds originating from humans production. Surface bacterial structures, cell wall and cytoplasmic membrane, surround each bacterial cell and create selective barriers between the cell interior and the outside world. They are a first site of contact between the cell and toxic compounds. Organic pollutants are able to penetrate into cytoplasmic membrane and affect membrane physiological functions. Bacteria had to evolve adaptation mechanisms to counteract the damage originated from toxic contaminants and to prevent their accumulation in cell. This review deals with various adaptation mechanisms of bacterial cell concerning primarily the changes in cytoplasmic membrane and cell wall. Cell adaptation maintains the membrane fluidity status and ratio between bilayer/nonbilayer phospholipids as well as the efflux of toxic compounds, protein repair mechanisms, and degradation of contaminants. Low energy consumption of cell adaptation is required to provide other physiological functions. Bacteria able to survive in toxic environment could help us to clean contaminated areas when they are used in bioremediation technologies.

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Adaptation mechanisms of bacteria during the degradation of polychlorinated biphenyls in the presence of natural and synthetic terpenes as potential degradation inducers

Cited by 18 publications

References 43 publications

Bacterial cell membrane adaptation responses on stress caused with the environmental pollutants

Bacterial cell membrane adaptation responses on stress caused with the environmental pollutants

The adaptation responses of bacterial cytoplasmic membrane fluidity in the presence of environmental stress factors — polychlorinated biphenyls and 3-chlorobenzoic acid

Response Mechanisms of Bacterial Degraders to Environmental Contaminants on the Level of Cell Walls and Cytoplasmic Membrane

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