Isolation and Typing of Canine Parvovirus in CRFK Cell Line in Puducherry, South India

Parthiban, S.; Mukhopadhyay, H. K.; Panneer, D.; Antony, P. X.; Pillai, R. M.

doi:10.1007/s12088-011-0105-6

Cited by 11 publications

(9 citation statements)

References 18 publications

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“…The six isolates also agglutinated feline RBCs, but agglutinated other erythrocytes less sensitively or negligibly. These results are consistent with those of other studies [ 22 , 23 ].…”

Section: Resultssupporting

confidence: 94%

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First Isolation of New Canine Parvovirus 2a from Tibetan Mastiff and Global Analysis of the Full-Length VP2 Gene of Canine Parvoviruses 2 in China

Zhong

Liang

Zhao

et al. 2014

IJMS

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Canine parvovirus 2 (CPV-2) was first identified in 1978, and is responsible for classic parvoviral enteritis. Despite the widespread vaccination of domestic carnivores, CPVs have remained important pathogens of domestic and wild carnivores. In this study, we isolated CPV-2 from Tibetan mastiffs and performed a global analysis of the complete VP2 gene sequences of CPV-2 strains in China. Six isolates were typed as new CPV-2a, according to key amino acid positions. On a phylogenetic tree, these six sequences formed a distinct clade. Five isolates occurred on the same branch as KF785794 from China and GQ379049 from Thailand; CPV-LS-ZA1 formed a separate subgroup with FJ435347 from China. One hundred ninety-eight sequences from various parts of China and the six sequences isolated here formed seven distinct clusters, indicating the high diversity of CPVs in China. Of 204 VP2 sequences, 183 (91.04%) encoded the mutation Ser297Ala, regardless of the antigenic type, implying that most Chinese CPV-2 strains contain the VP2 mutation Ser297Ala. However, the biological significance of this change from prototype CPV-2a/2b to new CPV-2a/2b types remains unclear. This study is the first to isolate new CPV-2a from the Tibetan mastiff. Our data show that new CPV-2a/2b variants are now circulating in China.

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“…The six isolates also agglutinated feline RBCs, but agglutinated other erythrocytes less sensitively or negligibly. These results are consistent with those of other studies [ 22 , 23 ].…”

Section: Resultssupporting

confidence: 94%

“…The virus-containing supernatants were used for DNA isolation or stored at –20 °C until further analysis. An HA test and TCID 50 test were used to characterize the viral isolates [ 22 , 23 ] . SCDN (Strain that isolated from Bulldog in Sichuan province) (CPV-2a) strain used as positive control [ 39 ].…”

Section: Methodsmentioning

confidence: 99%

First Isolation of New Canine Parvovirus 2a from Tibetan Mastiff and Global Analysis of the Full-Length VP2 Gene of Canine Parvoviruses 2 in China

Zhong

Liang

Zhao

et al. 2014

IJMS

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“…Age-wise prevalence was found to be more between the age group of 3-6 months (below 6 months), followed by age group of 1-3 months, 6-12 months and the least above 12 months. This higher prevalence rate below 6 months also reported earlier [ 8 , 17 - 19 ]. The higher incidence of CPV below 6 months might be due to the affinity of the virus for rapidly multiplying intestinal crypt cells in weaning pups with higher mitotic index due to changes in bacterial flora as well as in the diet due to weaning [ 20 , 21 ].…”

Section: Discussionsupporting

confidence: 88%

Epidemiological study of canine parvovirus infection in and around Bhubaneswar, Odisha, India

Behera¹,

Panda²,

Sahoo³

et al. 2015

Vet World

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Aim:An epidemiological study of canine parvovirus infection in dogs in and around Bhubaneswar, Odisha was conducted between December 2012 to March 2013 and prevalence rate was studied on the basis of age, breed, and sex.Materials and Methods:A total of 71 fecal samples from suspected diarrheic dogs were collected in sterile phosphate buffer saline (10% W/V) and examined by polymerase chain reaction (PCR) for detection of canine parvo virus infection, followed by epidemiological study in relation to age, breed, and sex.Results:Of 71 samples analyzed, 29 (40.85%) were found to be positive by PCR assay. The infection was higher in Deshi/local breeds (34.48%), followed by German shepherd (17.24%), equal incidences in mixed and Labrador retriever (10.34%), Rottweiler and German spitz showed 6.90% each and finally lower incidences in four breeds (3.45%) such as Dalmatians, Nea politan mastiff, Pug and Great Dane. Age-wise prevalence study revealed the infection being more in the age group of 3-6 months (41.37%), followed by equal incidences of 27.59% in 1-3 months and 6-12 months age group, and a low incidence in age groups above 12 months (3.45%). The incidence was predominantly higher in males (86.21%) than females (13.79%).Conclusions:The epidemiological analysis revealed that the breed wise prevalence was found to be more in Deshi breeds as compared to others, age groups below 6 months were found to be more prone to parvovirus infection and males were mostly infected.

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“…In addition, our study showed that CPV isolation is feasible when faeces are processed shortly after collection. This procedure allows for enriching viral preparations for several purposes, including biological studies of viral replication, drug susceptibility and virus-host interactions ( Decaro and Buonavoglia, 2012 ; Parthiban et al, 2011 ). In a recent study, rural dogs living in households around six Atlantic forests in southeastern Brazil were serologically tested for CPV antibodies and 97% were positive ( Curi et al, 2016 ).…”

Section: Resultsmentioning

confidence: 99%

Circulation of canine parvovirus among dogs living in human-wildlife interface in the Atlantic forest biome, Brazil

Vieira¹,

Hoffmann²,

Fabri³

et al. 2017

Heliyon

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Despite of the role of domestic dogs as reservoirs for threatening viral diseases for wild carnivores, few studies have focused to identify circulation of viruses among dogs living in human/wildlife interfaces. To identify canine parvovirus (CPV) types circulating in dogs living in an Atlantic forest biome, faecal samples (n = 100) were collected at the same period (one week) corresponding to each of four areas, during 2014 to 2016 and corresponded to 100 different individuals. CPV was isolated in cell culture from 67 out 100 (67%) samples from healthy dogs. Cytopathic effects were characterized by total or partial cell culture lysis. Genome sequences of CPV-2a (10%), CPV-2b (7%) and CPV-2c (50%) were concomitantly detected by PCR and nucleotide sequencing. The current study addresses the importance of monitoring CPV circulation among dogs presenting potential contact with wildlife species.

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Isolation and Typing of Canine Parvovirus in CRFK Cell Line in Puducherry, South India

Cited by 11 publications

References 18 publications

First Isolation of New Canine Parvovirus 2a from Tibetan Mastiff and Global Analysis of the Full-Length VP2 Gene of Canine Parvoviruses 2 in China

First Isolation of New Canine Parvovirus 2a from Tibetan Mastiff and Global Analysis of the Full-Length VP2 Gene of Canine Parvoviruses 2 in China

Epidemiological study of canine parvovirus infection in and around Bhubaneswar, Odisha, India

Circulation of canine parvovirus among dogs living in human-wildlife interface in the Atlantic forest biome, Brazil

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