Isolation and identification of Indonesian Lactobacillus reuteri strain from the saliva of young adults

Widyarman, Armelia Sari; Pranoto, Stella; Theodorea, Citra Fragrantia; Bachtiar, Endang Winiati

doi:10.26912/sdj.v2i2.2840

Cited by 6 publications

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“…The evolution gap between two bacterial strains can be measured using their nucleotide gene identity through BLAST, with average nucleotide identity (ANI) of 95-98% [32]. Lactobacillus reuteri LC 382415 similarity to L. reuteri ATCC 55730, as suggested by an ANI of 96% [21].…”

Section: Discussionmentioning

confidence: 99%

“…Streptococcus mutans (ATCC ® 25175™) and S. sanguinis (ATCC ® 10556™) were cultured in brain heart infusion broth in anaerobic conditions at 37°C for 24 h. Lactobacillus reuteri LC382415 [21] was cultured in de Man, Rogosa, and Sharpe broth in anaerobic conditions at 37°C for 72 h. For L. reuteri with glycerol supplementation, glycerol (250 mM) was added to L. reuteri suspensions.…”

Section: Bacterial Culturementioning

confidence: 99%

“…Previously, the first Indonesian strain of L. reuteri, obtained from GenBank with accession number LC382415, was found in the saliva of Indonesian healthy young adults [21]. However, its mechanism of action in dental caries prevention has not yet been reported.…”

Section: Introductionmentioning

confidence: 99%

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Indonesian Strain of Lactobacillus reuteri Probiotic Reduces the Initial Biofilm Colonization

Widyarman¹,

Ramajayanti²,

Theodorea³

2020

TODENTJ

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Background: The benefits of probiotics for human health have long been proven. Probiotic Lactobacillus reuteri, can produce a beneficial broad-spectrum antibacterial compound called reuterin by metabolizing glycerol. Objective: The aim of the study was to investigate the effect of the Indonesian strain of L. reuteri LC382415 on mono- and dual-species Streptococcus mutans and Streptococcus sanguinis biofilms in vitro. Methods: Streptococcus mutans and S. sanguinis were cultured in BHI broth. Lactobacillus reuteri LC382415 was inoculated on MRS agar. The different concentrations effect of L. reuteri (1×104, 1×106, and 1×108 CFU/mL) with and without glycerol supplementation on microbial biofilms were examined using a biofilm assay after incubation for 1,3,6, and 24-h. The biofilm mass optical density was measured with a microplate spectrophotometer at 490 nm. Chlorhexidine gluconate (0.2%) was used as a positive control, and wells without treatment were used as negative controls. Results: A significant reduction in mono- and dual-species S. mutans and S. sanguinis biofilm formation was observed after treatment with all concentrations of L.reuteri and after all incubation periods (p<0.05) with or without glycerol supplementation. The concentration of 1×104 CFU/mL after 3-h incubation was the most effective in inhibiting biofilm formation, with 87.8% S. mutans, 95.9% S. sanguinis, and 80.4% dual-species biofilm reduction compared to the negative control (p<0.05). Conclusion: The Indonesian strain of L. reuteri effectively reduces mono- and dual-species S.mutans and S. sanguinis biofilms. This suggests that it may be useful in preventing biofilm formation in oral cavities. Future studies on the mechanism of action of this active component are warranted.

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Section: Discussionmentioning

confidence: 99%

Section: Bacterial Culturementioning

confidence: 99%

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Indonesian Strain of Lactobacillus reuteri Probiotic Reduces the Initial Biofilm Colonization

Widyarman¹,

Ramajayanti²,

Theodorea³

2020

TODENTJ

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“…4 Probiotics are products that contain specific strains of bacteria that are beneficial for health. 5 L. reuteri DSM 17938 is a probiotic which is well-known to have benefits for several digestive diseases. 6 L. reuteri DSM 17938 is a daughter strain of Lactobacillus reuteri ATCC 55730.…”

Section: Bioscientia Medicina: Journal Of Biomedicine and Translation...mentioning

confidence: 99%

Lactobacillus Reuteri DSM 17938 in Infantile Colic: A Systematic Review and Meta-Analysis

2022

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Background. Infantile colic, defined as paroxysmal, excessive, and consolable crying without an identifiable cause. It is common in the first 3 months of life. There have been many RCT studies conducted. A systematic synthesis is required to summarize the results of these RCTs. This study was aimed to systematically analyze the benefits of Lactobacillus reuteri DSM 17938 for infantile colic. Methods. This systematic review and meta-analysis used keywords: (“infantile colic” OR “abdominal cramps” OR “abdominal cramp” OR “infant colic”) AND “lactobacillus reuteri” AND (placebo OR placebos) AND (“randomized controlled trial” OR RCT). The databases used were: pubmed, science direct, and google scholar. Meta-analysis was conducted to combine the articles. The eligibility criteria were: patient with infantile colic, intervention with L. reuteri DSM 17938, control was placebo, RCT study design, outcome with dichotomous scale, all races, all ethnicities, all countries, all genders, in English, and not limited by year. Results. The search resulted in 800 articles. After reducing duplication, the number of articles was 747. Screening with titles and abstracts resulted in 13 full teks articles. Six articles fitted the eligibility criteria. The results of the meta-analysis were as follows. The forest plot showed that the combined effect of the six articles showed an RR of 0.47 and was statistically significant (p <0.001). Conclusion. Based on the results of this meta-analysis, L. reuteri DSM 17938 is recommended for infants with infantile colic. There were no significant side effects with the use of L. reuteri DSM 17938 on infantile colic.

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“…10,19 L. reuteri has been isolated from the saliva of Indonesian subjects and is registered in the Genbank with accession number LC 382415. 20 Previous studies indicated that L. reuteri DSM 17938 and L. reuteri PTA 5289 have antifungal activity against five of six Candida species commonly found in the oral cavity (i.e., C. albicans, C. dubliniensis, C. glabrata, C. parapsilosis, and C. tropicalis.)…”

Section: Introductionmentioning

confidence: 99%

Persistent Endodontics Pathogens Biofilm Inhibited by Lactobacillus reuteri Indonesian Strain Lactobacillus reuteri Indonesian

Widyarman¹,

Lazaroni²

2019

J Dent Indones

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Lactobacillus reuteri is known as probiotics that effective to improve oral health. Objective: To analyze the effect of L. reuteri Indonesian strain, towards Enterococcus faecalis and Candida albicans biofilm growth. Methods: This study was conducted using biofilm assay. Each E. faecalis ATCC-29212 and C. albicans ATCC-10231 were cultured in Brain Heart Infusion Broth, distributed into 96 well-microplate and incubated for 24h, 37°C, anaerobic condition for E. faecalis and aerobic condition for C. albicans. L. reuteri LC382415 (Indonesian strain) was cultured in de-Mann Rogosa Sharpe Broth and diluted into different concentrations (10 8 ,10 6 , and 10 4 CFU/mL) subsequently, each concentration distributed into biofilm well. Biofilm wells without probiotic was used as negative control. Biofilm mass were measured using crystal-violet dye at 490nm using microplate-spectrophotometer. Data was statistically analyzed by one-way ANOVA test, statistical significance set as p<0.05. Results: Significant reduction of biofilm growth of E. faecalis and C. albicans after treatment with L. reuteri. The ideal concentration was found at L. reuteri 10 8 CFU/mL with 79.2% E. faecalis biofilm reduction and L. reuteri 10 4 CFU/mL with 62.5% C. albicans biofilm reduction compared to control (p<0.05) set at 100%. The ANOVA test results showed that L. reuteri in all concentrations and all time periods in this study had the ability to inhibit biofilm growth of both species (p<0.05) compared to negative control. Conclusion: L. reuteri Indonesian strain inhibit the biofilm growth of E. faecalis and C. albicans. This antibiofilm effect may be useful in preventing biofilm growth in root canal.

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Isolation and identification of Indonesian Lactobacillus reuteri strain from the saliva of young adults

Cited by 6 publications

References 0 publications

Indonesian Strain of Lactobacillus reuteri Probiotic Reduces the Initial Biofilm Colonization

Indonesian Strain of Lactobacillus reuteri Probiotic Reduces the Initial Biofilm Colonization

Lactobacillus Reuteri DSM 17938 in Infantile Colic: A Systematic Review and Meta-Analysis

Persistent Endodontics Pathogens Biofilm Inhibited by Lactobacillus reuteri Indonesian Strain Lactobacillus reuteri Indonesian

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