Isolation and characterization of mycorrhizal fungi in Cyrtopodium vernum Rchb. F. &amp; Warm (Orchidaceae)

Gonçalves, Francisco César; Nunes, Camila de Marillac Costa; Filippi, Marta Cristina Corsi de; Araújo, Leila Garcês de; Gonçalves, Letícia de Almeida; Sibov, Sérgio Tadeu

doi:10.4322/rca.ao1359

Cited by 3 publications

(6 citation statements)

References 11 publications

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“…1a) coletadas em ambiente natural. Nas observações em microscopia óptica (MO) o preparo dos cortes histológicos e a visualização dos fungos micorrízicos foram realizados de acordo com Gonçalves et al (2014). Arditti (1977), MS modificado (acrescido de hormônios) e MS (Suplemento 1).…”

Section: Visualização De Fungos Nas Raízesunclassified

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Cultivo in vitro de Epidendrum nocturnum (Orchidaceae) ocorrente no Cerrado da região Centro-Oeste

et al. 2016

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ResumoAs orquídeas podem ser propagadas in vitro em meios de cultivo assépticos ou em simbiose com fungos micorrízicos. Epidendrum nocturnum ocorre em áreas de Cerrado e neste estudo objetivou-se a visualização de fungos micorrízicos em suas raízes bem como o cultivo assimbiótico de suas plântulas em diferentes meios e a micorrização in vitro. No desenvolvimento assimbiótico testou-se três meios de cultura em um ensaio e, em outro ensaio, foram testadas formulações alternativas do meio Knudson C modificado, com a adição de polpa de frutas. Fungos micorrízicos de orquídeas foram usados na micorrização in vitro. Pelotons foram visualizados nas raízes de E. nocturnum. O meio Knudson C modificado diferiu estatisticamente dos demais, nas duas intensidades luminosas, no primeiro ensaio. No segundo ensaio, após sete meses, os meios com formulação alternativa não diferiram estatisticamente entre si. Na micorrização in vitro as plântulas com o isolado fúngico En07 (Waitea circinata) apresentaram maior vigor visual em relação aos demais tratamentos, mas não houve diferença estatística com o controle. Os meios com formulação alternativa e o Knudson C modificado podem ser utilizados no desenvolvimento in vitro de E. nocturnum e o isolado En07 pode ser usado na micorrização visando estratégias de conservação desta orquídea. Palavras-chave: orquídea, desenvolvimento assimbiótico, micorrização, fungo. AbstractOrchids can be propagated in vitro in aseptic culture media or in symbiosis with mycorrhizal fungi. Epidendrum nocturnum occurs in areas of Cerrado and this study aimed to mycorrhizal fungi visualization in their roots and its asymbiotic growing their seedlings in different media and in vitro mycorrhization. In asymbiotic development was tested three culture in a test and, another test, alternative formulations of medium Knudson C modified with addition of fruit pulp. Mycorrhizal fungi orchids were used in in vitro mycorrhization. Pelotons were visualized in the roots of E. nocturnum. The medium Knudson C modified statistically different from the others, the two light intensities, in the first test. In the second test, after seven months, with the alternative formulation means were not statistically different from each other. In vitro mycorrhization plantlets with isolated fungal En07 (Waitea circinata) showed greater visual effect compared to other treatments, but there was no statistical difference with control. The media with alternative formulation and Knudson C modified can be used in vitro development of E. nocturnum, and the isolated fungal En07 can be used in order mycorrhization of this orchid conservation strategies.

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Section: Visualização De Fungos Nas Raízesunclassified

“…Os principais fungos micorrízicos obtidos de orquídeas no Brasil pertencem aos gêneros Rhizoctonia sp., Epulorhiza sp., e Ceratorhiza sp. (Nogueira et al 2005;Pereira et al 2011;Gonçalves et al 2014).…”

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Cultivo in vitro de Epidendrum nocturnum (Orchidaceae) ocorrente no Cerrado da região Centro-Oeste

et al. 2016

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“…In Brazil, many isolated fungi from orchids belong to this respective anamorphic, the Epulorhiza genus, obtained from Epidendrum dendrobioides and Sophronitis milleri (Nogueira et al 2005), Epidendrum secundum (Pereira et al 2009;Nogueira et al 2014), Acianthera limae and Polystachya concreta (Nogueira et al 2014) and Cyrtopodium vernum (Gonçalves et al 2014).…”

Section: Isolation and Identification Of Mycorrhizal Fungusmentioning

confidence: 99%

Cyrtopodium paludicolum germination with two Tulasnella isolates

et al. 2017

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Symbiosis between orchid seeds and mycorrhizal fungi has been reported to be a determining factor in the success of germination and protocorm development in vitro. Th e aim of this study was to isolate and identify by molecular analysis the mycorrhizal fungus associated with Cyrtopodium paludicolum, and to evaluate its effi ciency in facilitating seed germination and development. Germination experiments were carried out using a fungus isolated from C. paludicolum (CH01) and Epidendrum secundum (M65), which has been successfully used a number of times in symbiotic germination. Th e experiments were conducted in a completely randomized design with treatments of CH01, M65 as well as under asymbiotic conditions. Th e mycobiont CH01 was successfully isolated from Cyrtopodium paludicolum and identifi ed as Tulasnella sp. Treatments with both fungi reached a higher germination percentage than under asymbiotic conditions, indicating no specifi city in the relationship between Cyrtopodium paludicolum and the fungi. Th e results presented have the potential to advance research into the propagation and conservation of C. paludicolum, a native of the Cerrado biome.

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“…Zettler et al (2007) reported symbiosis maintenance between Tulasnella fungi and orchid S. brevilabris from embryo stage until adult phase. In the same way, Látalová & Baláz (2010) and Gonçalves et al (2014) demonstrated interaction between orchid and mycorrhizal fungus from protocorm until adult stage. Silva et al (2016) observed that W. circinata increases the vigor of Epidendrum nocturnum seedlings in vitro and a potential interaction was observed with seeds and seedlings (in vitro and ex vitro, respectively) of C. saintlegerianum too.…”

Section: Discussionmentioning

confidence: 63%

“…The mycorrhizal fungi were isolated according to Gonçalves et al (2014) using the PDA medium (Potato Dextrose Agar, composed of 200 g of potato, 20 g of dextrose, 20 g of agar and 1 L of water - Otero et al 2004). Fungal isolates were cultivated on PDA plates under continuous fluorescent light for five days at room temperature (26±2 °C).…”

Section: Mycorrhizae Microscopy Characterization Fungal Isolation Anmentioning

confidence: 99%

Seed germination and development of orchid seedlings (Cyrtopodium saintlegerianum) with fungi

et al. 2019

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Fungi of Rhizoctonia complex are mycorrhizal of orchids and may to germinate yours seeds and development the seedlings. In this context, our objective was to select a fungal isolate to promote seed germination and seedling development of Cyrtopodium saintlegerianum. Pelotons were found in the roots and three mycorrhizal fungi were isolated. We tested mycorrhizal isolates obtained from C. saintlegerianum roots and six mycorrhizal fungi from other orchids as well three pathogenic isolates (of rice and bean) to germinate the seeds in oatmeal-agar medium. Seeds not inoculated were used as control. The isolates En07 (Waitea circinata), Cs10 (Tulasnella sp.) and Ro88 (Rhizoctonia oryzae) were efficient to promote seed germination, but only En07 differing statistically of the control. The non-specific isolate En07 promoted germination in 81% of seeds and the specific isolate (Cs10) promoted 60%, evidencing the non-specificity mycorrhizal association in this orchid during germination. Axenic seedlings were inoculated with four mycorrhizal fungi (non-inoculated seedlings - control). After six months, the isolates En07 and Cs10 were efficient in the interaction with the seedlings, but did not differ to the control. Therefore, our results suggested that fungi of the Rhizoctonia complex can be used in the germination and seedling development of C. saintlegerianum.

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Isolation and characterization of mycorrhizal fungi in Cyrtopodium vernum Rchb. F. & Warm (Orchidaceae)

Cited by 3 publications

References 11 publications

Cultivo in vitro de Epidendrum nocturnum (Orchidaceae) ocorrente no Cerrado da região Centro-Oeste

Cultivo in vitro de Epidendrum nocturnum (Orchidaceae) ocorrente no Cerrado da região Centro-Oeste

Cyrtopodium paludicolum germination with two Tulasnella isolates

Seed germination and development of orchid seedlings (Cyrtopodium saintlegerianum) with fungi

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