2005
DOI: 10.11598/btb.2005.0.25.211
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Isolation and Characterization of Mannanolytic Thermophilic Bacteria From Palm Oil Shell and Their Mannanase Enzyme Production Properties

Abstract: A mannanolytic thermophilic bacterium (L-07) was isolated from palm oil shell after 2 days of enrichment in liquid medium supplemented with 1% palm kernel meal as mannan source. Sequence analysis of 16S-rRNA indicated that L-07 was similar (98%) to Geobacillus stearothermophilus, a species of thermophilic aerobic bacteria. We found that G. stearothermophilus L-07 produced extracellular β-1,4-mannanases, but no β-manosidase and α-galactosidase activities. The growth of L-07 reached its maximum (3.0 x 10 6 cell/… Show more

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Cited by 6 publications
(9 citation statements)
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“…Observations were made included mannanolytic index and bacterial staining. Selected colony with highest clear zones was tested using enzymatic assay with DNS method to determine the length of incubation time for enzyme production 15 . salt tolerance test Mannanolytic isolates were further tested for their resistance to salt content.…”
Section: Bacteria Selection For Mannanolytic Activitymentioning
confidence: 99%
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“…Observations were made included mannanolytic index and bacterial staining. Selected colony with highest clear zones was tested using enzymatic assay with DNS method to determine the length of incubation time for enzyme production 15 . salt tolerance test Mannanolytic isolates were further tested for their resistance to salt content.…”
Section: Bacteria Selection For Mannanolytic Activitymentioning
confidence: 99%
“…Bacteria were grown on SWC agar with the addition of salt as much as 0%, 3%, and 6% NaCl. The size of the colony was observed after an incubation period of 24 hours at room temperature 15 . ph tolerance test Mannanolytic isolates were tested for their resistance to media pH stress.…”
Section: Bacteria Selection For Mannanolytic Activitymentioning
confidence: 99%
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