Isolation and characterization of DNA aptamers against Escherichia coli using a bacterial cell–systematic evolution of ligands by exponential enrichment approach

Kim, Yeon Seok; Song, Min Young; Jurng, Jongsoo; Kim, Byoung Chan

doi:10.1016/j.ab.2013.01.014

Cited by 101 publications

(58 citation statements)

References 36 publications

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“…The best performance is attributed to the Ag@Au-aptasensor and there is a linear relationship between peak current and the concentration of E. coli to 10 4 CFU/mL. For higher concentrations, the current no longer changes significantly because of the saturation of the surface [29]. The calculated LOD for this aptasensor is around 90 CFU/mL, while the reported LOD in the literature for this aptamer cocktail is 370 CFU/mL at the surface of a gold electrode [28].…”

Section: Electrochemical Detection Of E Coli and The Role Of Ag@au Nmentioning

confidence: 82%

Unique Properties of Core Shell Ag@Au Nanoparticles for the Aptasensing of Bacterial Cells

et al. 2016

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Abstract:In this article, it is shown that the efficiency of an electrochemical aptasensing device is influenced by the use of different nanoparticles (NPs) such as gold nanoparticles (Au), silver nanoparticles (Ag), hollow gold nanospheres (HGN), hollow silver nanospheres (HSN), silver-gold core shell (Ag@Au), gold-silver core shell (Au@Ag), and silver-gold alloy nanoparticles (Ag/Au). Among these nanomaterials, Ag@Au core shell NPs are advantageous for aptasensing applications because the core improves the physical properties and the shell provides chemical stability and biocompatibility for the immobilization of aptamers. Self-assembly of the NPs on a cysteamine film at the surface of a carbon paste electrode is followed by the immobilization of thiolated aptamers at these nanoframes. The nanostructured (Ag@Au) aptadevice for Escherichia coli as a target shows four times better performance in comparison to the response obtained at an aptamer modified planar gold electrode. A comparison with other (core shell) NPs is performed by cyclic voltammetry and differential pulse voltammetry. Also, the selectivity of the aptasensor is investigated using other kinds of bacteria. The synthesized NPs and the morphology of the modified electrode are characterized by UV-Vis absorption spectroscopy, scanning electron microscopy, energy dispersive X-ray analysis, and electrochemical impedance spectroscopy.

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Section: Electrochemical Detection Of E Coli and The Role Of Ag@au Nmentioning

confidence: 82%

Unique Properties of Core Shell Ag@Au Nanoparticles for the Aptasensing of Bacterial Cells

et al. 2016

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“…However, due to outer cell surface complexity, molecular level understanding of how these aptamers bind on E. coli strains is still unknown. Using, the same aptamers could bind to cell surface components competitively followed by fluorescence emission; therefore, two or more different aptamers could be assumed to bind non-competitively (Kim et al 2013c). …”

Section: Escherichia Colimentioning

confidence: 99%

“…Cell-SELEX provides flexibility in the detection of various cell types (bacteria, viruses, cancer cells) and is now used in the identification of the unknown cells' surface biomarkers (Kim et al 2013c). Recently, modified SELEX protocols based on traditional SELEX method have significantly improved to advance the application of aptamer technology in various fields (Xu et al 2011).…”

Section: Introductionmentioning

confidence: 99%

An Update on Aptamer-Based Multiplex System Approaches for the Detection of Common Foodborne Pathogens

et al. 2017

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Foodborne ailments constitute a public health challenge and pose an incredible economic burden in healthcare system around the globe. This dilemma has urged authorities and other entities working in field of food quality control and supply chain to play a pivotal role in ensuring food safety. Analytical strategies have been developed using numerous systematic evolution of ligands by exponential enrichment (SELEX) methods to assure food safety. High-affinity and high-sensitivity ssDNA and RNA aptamers against pathogens have emerged as a novel strategy, as compared to the more resource-demanding and complicated biochemical test-based approaches. Thus, this review aims to focus on some methods used in the selection of specific bare, modified, and conjugated aptamers and on the further analysis of selected aptamers using flow cytometer or post-SELEX modifications for enhanced detection of frequently diagnosed foodborne bacteria such as Bacillus sp., Campylobacter jejuni, Escherichia sp., Salmonella sp., Staphylococcus aureus, Shigella sp., Listeria monocytogenes, and Streptococcus pyogenes and/or targeting their cell components towards attaining fast, sensitive, and selective methods for the detection of pathogens in food(s) or other sources.

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“…The FNP had excitation and emission maxima of 540 and 560 nm, respectively. Next, our previously described 3′-amino-modified E. coli-specific aptamer (5′-GCA ATG GTA CGG TAC TTC CCC ATG AGT GTT GTG AAA TGT TGG GAC ACT AGG TGG CAT AGA GCC GCA AAA GTG CAC GCT ACT TTG CTA A-3′) was added to the solution at a final concentration of 750 nM (Kim et al, 2013). The resulting mixture was incubated at room temperature for an additional 3 h in a ThermoMixer (Eppendorf, Germany) to allow time for the conjugation of aptamers to microspheres.…”

Section: Preparation Of A-fnpsmentioning

confidence: 99%

“…We used an E. colispecific ssDNA aptamer, which we described in a previous study and which had a dissociation constant of 25.2 nM for E. coli (Kim et al, 2013). A-FNPs were analyzed by DLS to clarify whether they dispersed well or aggregate in solution.…”

Section: A-fnps For E Coli Detectionmentioning

confidence: 99%

Fast and continuous microorganism detection using aptamer-conjugated fluorescent nanoparticles on an optofluidic platform

Chung

Kang

Jurng

et al. 2015

Biosensors and Bioelectronics

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Isolation and characterization of DNA aptamers against Escherichia coli using a bacterial cell–systematic evolution of ligands by exponential enrichment approach

Cited by 101 publications

References 36 publications

Unique Properties of Core Shell Ag@Au Nanoparticles for the Aptasensing of Bacterial Cells

Unique Properties of Core Shell Ag@Au Nanoparticles for the Aptasensing of Bacterial Cells

An Update on Aptamer-Based Multiplex System Approaches for the Detection of Common Foodborne Pathogens

Fast and continuous microorganism detection using aptamer-conjugated fluorescent nanoparticles on an optofluidic platform

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