Fast and continuous microorganism detection using aptamer-conjugated fluorescent nanoparticles on an optofluidic platform

Chung, Jinyang; Kang, Joon Sang; Jurng, Jongsoo; Jung, Jae Hee; Kim, Byoung Chan

doi:10.1016/j.bios.2014.08.039

Cited by 49 publications

(30 citation statements)

References 31 publications

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“…The accumulative effect of different nanomaterials also can lead to dramatic improvements in sensitivity. Sensing platforms based upon multiple nanotechniques have been reported, combining SERS, electrochemistry, microfluidics, and fluorescence for more sensitive and quantitative detection (23). Major nanomaterial-based detection techniques, time to results, sensitivity, sample preparation required, and matrices in which these techniques have been evaluated are summarized in Table 1.…”

Section: Detection Methods Based On Nanomaterialsmentioning

confidence: 99%

Recent Advancements in Nanobioassays and Nanobiosensors for Foodborne Pathogenic Bacteria Detection

Chen

Park

2016

Journal of Food Protection

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Bacterial pathogens are one of the leading causes of food safety incidents and product recalls worldwide. Timely detection and identification of microbial contamination in agricultural and food products is crucial for disease prevention and outbreak investigation. In efforts to improve and/or replace time-consuming and laborious "gold standards" for pathogen detection, numerous alternative rapid methods have been proposed in the past 15 years, with a trend toward incorporating nanotechnology and nanomaterials in food pathogen detection. This article is a review of the use of nanotechnology in various detection and sample preparation techniques and advancements in nanotechnology applications in food matrices. Some practical considerations in nanobioassay design are discussed, and the gaps between research status quo and market demands are identified.

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Section: Detection Methods Based On Nanomaterialsmentioning

confidence: 99%

Recent Advancements in Nanobioassays and Nanobiosensors for Foodborne Pathogenic Bacteria Detection

Chen

Park

2016

Journal of Food Protection

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“…65 One of these aptamers (E2) was conjugated with fluorescent nanoparticles (A-FNPs) and was incorporated into an optofluidic particle-sensor platform for high-throughput real-time and single-cell detection of E.coli . 66 Aptamer E2 was then employed by Jin et al to develop a detection platform based on fluorescence resonance energy transfer (FRET) for rapid, ultrasensitive and specific detection of bacteria. 67 Another aptamer against E. coli KCTC 2571, called aptamer E1, was applied to label-free and highly sensitive electro chemical detection of E. coli on the basis of rolling circle amplifications coupled peroxidase-mimicking DNAzyme amplification, with the detection limit of 8 CFU/mL.…”

Section: E Colimentioning

confidence: 99%

Oligonucleotide aptamers: promising and powerful diagnostic and therapeutic tools for infectious diseases

Pan

Liu

Zhang

2018

Journal of Infection

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The entire human population is at risk of infectious diseases worldwide. Thus far, the diagnosis and treatment of human infectious diseases at the molecular and nanoscale levels have been extremely challenging tasks because of the lack of effective probes to identify and recognize biomarkers of pathogens. Oligonucleotide aptamers are a class of small nucleic acid ligands that are composed of single-stranded DNA (ssDNA) or RNA and act as affinity probes or molecular recognition elements for a variety of targets. These aptamers have an exciting potential for diagnose and/or treatment of specific diseases. In this review, we highlight areas where aptamers have been developed as diagnostic and therapeutic agents for both bacterial and viral infectious diseases as well as aptamer-based detection.

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“…Moreover, to achieve specific detection, fluorophores are usually modified with a recognition agent, e.g. antibodies [100–102] and aptamers [40]. However, conjugation with fluorophores can in some cases interfere with protein function, causing the antibody to lose affinity.…”

Section: Fluorescence Spectroscopymentioning

confidence: 99%

Optical Biosensing of Bacteria and Bacterial Communities

Bohn

2017

J. Anal. Test.

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Bacterial sensing is important for understanding the numerous roles bacteria play in nature and in technology, understanding and managing bacterial populations, detecting pathogenic bacterial infections, and preventing the outbreak of illness. Current analytical challenges in bacterial sensing center on the dilemma of rapidly acquiring quantitative information about bacteria with high detection efficiency, sensitivity, and specificity, while operating within a reasonable budget and optimizing the use of ancillary tools, such as multivariate statistics. This review starts from a general description of bacterial sensing methods and challenges, and then focuses on bacterial characterization using optical methods including Raman spectroscopy and imaging, infrared spectroscopy, fluorescence spectroscopy and imaging, and plasmonics, including both extended and localized surface plasmon resonance spectroscopy. The advantages and drawbacks of each method in relation to the others are discussed, as are their applications. A particularly promising direction in bacterial sensing lies in combining multiple approaches to achieve multiplex analysis, and examples where this has been achieved are highlighted.

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Fast and continuous microorganism detection using aptamer-conjugated fluorescent nanoparticles on an optofluidic platform

Cited by 49 publications

References 31 publications

Recent Advancements in Nanobioassays and Nanobiosensors for Foodborne Pathogenic Bacteria Detection

Recent Advancements in Nanobioassays and Nanobiosensors for Foodborne Pathogenic Bacteria Detection

Oligonucleotide aptamers: promising and powerful diagnostic and therapeutic tools for infectious diseases

Optical Biosensing of Bacteria and Bacterial Communities

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