Isolation and characterization of antimicrobial activity conferring component(s) from seeds of bitter gourd (Momordica charantia)

Objective: To isolate the antibacterial proteins/peptides from Ficus glomerata leaf.Methods: Present study was designed to investigate antibacterial activity of proteins/peptides isolated from Ficus glomerata leaf. The isolated proteins/peptides were further checked for antibacterial activity against, Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli and Salmonella entrica bacterial pathogens. Results:The results indicates that a 35kDa of protein were identified and exhibit good antibacterial activity against bacterial pathogen among all strains, Salmonella entrica and Pseudomonas aeruginosa exhibit good results with a clear zone of inhibition. Conclusion:Ficus glomerata is popular for its medicinal properties against therapeutic potential. In the present study a novel protein with broad spectrum antibacterial activity. Microbes cause severe damage to plants which results in a large economic loss so; this protein can be use as an active agent in agriculture for plant protection and also in the development of novel therapeutic agents.

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“…Application of medicinal plants and their products for the treatment of various human afflictions had been made by man since ancient time [1].…”

Section: Introductionmentioning

confidence: 99%

Isolation of Antibacterial Protein/Peptide From Ficus Glomerata Leaf

Thapliyal¹,

Bisht²,

Singh³

2016

Int J Curr Pharm Sci

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“…66 The aqueous seed extract has shown greater antimicrobial capacity by inhibiting the growth of Fusarium solani 19 and Pasteurella multocida, compared to the extracts of methanolic, ethanolic, hexane and ethyl acetate that were effective against S. aureus, Enterococcus and fungi. 33,[94][95][96] Seed oil, with t-nerolidol, c-dihydrocarveol and germacrene ( Figure 3) as its main constituents, has shown antimicrobial activity towards S. aureus, E. coli and C. albicans 75,97 , which makes the development of green antibacterial soaps, without chemical aggregates, feasible. 15 The levels of flavonoids and phenols such as catechin, myricetin, quercetin, gallic acid, chlorogenic acid, gentisic acid and salicylic acid, increase considerably in hair roots in vitro growth compared to unprocessed roots, although metabolites such as ferulic acid, rutin, naringenin and naringin decreased significantly in the hair roots.…”

Section: 642mentioning

confidence: 99%

Antimicrobial Activity and Chemical Composition of Momordica Charantia: A Review

Torre¹,

Sagástegui-Guarniz²,

Silva-Correa³

et al. 2020

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Bitter melon, bitter cucumber or bitter gourd are some of the names given to M. charantia. It belongs to the Cucurbitaceae family. 44 M. charantia is a vegetable with many culinary uses, especially in Asia and Africa, and is commonly cultivated in Africa, India, Malaysia, China and South America. 44,45 M.ABSTRACT Momordica charantia L. (bitter melon) is a plant belonging to the Cucurbitaceae family and is widely distributed in tropical and subtropical areas around the world, mainly in Asia, India, China and Brazil, where it is traditionally used as a medicinal plant, and the fruits of some varieties of M. charantia are consumed as food. Studies have determined that this plant contains a great diversity of bioactive compounds with therapeutic potential like charantin, α-momorcharin and MAP30, and highlighting its properties as antidiabetic, antiulcer, antioxidant, antimicrobial, anthelmintic, antihyperglycemic and anticancer. Review shows the complete botanical description of the plant (fruits, leaves, stem, etc.), the bioactive chemical compounds reported in the plant species, the antimicrobial activity of the extracts or fractions of M. charantia, emphasizing the antibacterial and antifungal activities, with respective values of MIC (Minimum Inhibitory Concentration) reported according to the methodology used in each study. The review seeks to update the phytochemical and pharmacological knowledge of M. charantia, which would be useful for researchers in their search for new chemical compounds of the plant, studies of its safety and efficacy, as well as the evaluation of its possible synergistic action in combination with other antimicrobials, in order to find new therapeutic alternatives against bacterial resistance.

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“…Aqueous extracts of seeds of M. charantia exhibited higher antibacterial activity as compared to its petroleum ether, methanolic and ethanolic extracts towards Staphylococcus aureus (Roopashree et al, 2008). Mahmood et al (2012) investigated the antimicrobial activity of M. charantia seed extracts against mammalian and poultry pathogens. The aqueous seed extract showed the highest antimicrobial activity against Pasteurella multocida while ethanol, n-hexane and petroleum ether extracts were effective against Staphylococcus aureus.…”

Section: Antimicrobial Activitymentioning

confidence: 99%