Isolation of Antibacterial Protein/Peptide From Ficus Glomerata Leaf

Thapliyal, Manisha; Bisht, Anjali; Singh, Ajeet

doi:10.22159/ijcpr.2016v8i4.15271

Cited by 12 publications

(5 citation statements)

References 24 publications

(15 reference statements)

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“…This similar result was carried out in leaf powder microscopy of both Ziziphus spina-christi and Ziziphus abyssinica [22]. Protein isolated from leaf of Ficus glomerata showed good antibacterial activity [23]. Microscopic observation of fruit powder show fragments of epidermal cells of pericarp and mesocarp.…”

Section: Discussionsupporting

confidence: 77%

Anatomical Studies of the Fruit of Ziziphus Rugosa

Prema¹,

Chitra²

2019

Asian J Pharm Clin Res

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Objective: The objective of this study was to study the anatomical features of the fruit Ziziphus rugosa. Methods: Surface view, longitudinal, transverse section (T.S), and powder microscopy of the fruits were studied. Results: The fruit is a drupe and shows vertical, irregular brown markings on the surface. The surface of the seed has irregular reticulate thick marking of brown. The longitudinal sections (L.S) of the fruit show thin green epicarp, wide soft mesocarp, and dark brown thick endocarp. In longitudinal view, the seed shows a thick conical part, thick wide shell which is hard. In the cotyledon is more or less cordate,white and soft with shallow notch at the upper end. In T.S, the fruit appears circular with soft pericarp, thick dark brown seed coat of sclereids, and vertically elongated white cotyledon. The epicarp layer is broken at certain places. In the mesocarp, some of the cells have dense tannin content and others have mucilage substance. The mucilaginous canals are wide, unbranched, and wavy. Some of the mesocarp cells contain dense accumulation of protein bodies. The sclerotesta contains palisade or macrosclereids. The seed consists of two elliptical, flat cotyledons which show dense accumulation of starch grains and small less prominent vascular strand. The powder microscopy of the fruit contains abundant dark mucilaginous substance. Fragments of epidermal cells of the pericarp and mesocarp cells are frequently seen. The seed coat epidermal cells are polyhedral with lignified cell walls. The circular brachy sclereids were often seen in the mesophyll tissue of the fruit. The ground parenchyma with various shapes and size is also noticed. Conclusion: This study revealed the presence of mucilaginous substance, tannin, sclereids, starch grains, and protein bodies. Therefore, this material will be efficient for eliminating some nutritional deficiency diseases.

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Section: Discussionsupporting

confidence: 77%

Anatomical Studies of the Fruit of Ziziphus Rugosa

Prema¹,

Chitra²

2019

Asian J Pharm Clin Res

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“…The resulting suspension was extensively dialyzed against distilled water. The dialyzed solutions were recovered and subjectedto further purification by chromatographic methods 13 .…”

Section: Extraction and Purification Of The Moth Beanmentioning

confidence: 99%

“…Antibacterial Assay: Antibacterial activity of the crude and purified peptide concentrates from seed sample was determined using Agar well diffusion method 13 against bacterial pathogens such as Staphylococcus aureus, Enterobacter faecalis, Salmonella typhimurium, Bacillus cereus, Vibrio cholerae, Shigella flexineri. The test organism (100ul) was inoculated on the surface of the Nutrient Agar medium.…”

Section: Sds-pagementioning

confidence: 99%

Untitled

2023

IJPSR

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Vigna aconitifolia L. represents a genus in the Fabaceae family and is one of the most drought-resistant legumes, commonly grown in arid and semi-arid regions of India. Therapeutic effects of Vigna aconitifolia L. have been reported in several publications, but there are only a few reports on the biological activities of the Vigna aconitifolia L. proteins. In the present study, antibacterial activities of the Vigna aconitifolia L., peptides have been investigated. Homogenized seed flour was pulverized, and its protein content was extracted in a phosphate buffer. After centrifugation, by adjusting pH, peptides from the supernatant were isolated. Gel filtration (G-25) and Ion-exchange chromatography on the seed extract gave fairly pure peptide fractions of which inhibitory activities were done by agar well diffusion method against Shigella flexineri, Bacillus cereus, Salmonella typhimurium, Staphylococcus aureus, Enterococcus faecalis, Vibrio cholerae bacterial pathogens. The results indicate that a 30kDa of the peptide was identified and exhibited good antibacterial activity against bacterial pathogens. Among all strains, Shigella flexineri and Staphylococcus aureus exhibit good results with a clear zone of inhibition.

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“…oryzae X. axanopodis pv. malvacearum 10 Ficus glomerata (Leaves) NA Protein 35 – S. entrica ND Thapliyal et al ( 2016 ) P. aeruginosa E. coli B. subtilis 11 Foeniculum vulgare Mill. (Seeds) Elute1 Protein mixture – S .…”

Section: Infectious Diseasesmentioning

confidence: 99%