Isolation and characterization of acid and pepsin-solubilized collagens from the skin of balloon fish (Diodon holocanthus)

Huang, Yu-Ru; Shiau, Chyuan‐Yuan; Chen, Huihuang; Huang, Bi-Chin

doi:10.1016/j.foodhyd.2011.02.011

Cited by 113 publications

(112 citation statements)

References 29 publications

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“…They agree with the literature where acetic acid was the best solvent for collagen extraction (Skierka & Sadowska, 2007). Previous works carried out in fish skin (Liu et al, 2012;Matmaroh, Benjakul, Prodpran, Encarnacion, & Kishimura, 2011;Nalinanon et al, 2007;Skierka & Sadowska, 2007;Zhang et al, 2007) showed that acid extractions yield lower amounts of collagen in comparison with the enzymatic method (Huang et al, 2011;Matmaroh et al, 2011;Singh, Benjakul, Maqsood, & Kishimura, 2011). Some pre-treatment parameters such as mechanical effect, pH, temperature and time could affect the yielding of collagen during extraction process.…”

Section: Yielding Of Extractionssupporting

confidence: 89%

“…Previous works carried out with different species such as fish showed the same behaviour; however, the range of temperature was observed between 25°C and 32°C. (Huang et al, 2011;Nalinanon et al, 2007;Safandowska & Pietrucha, 2013;Yan et al, 2008;Zhang et al, 2007). The thermal stability of collagen obeys to the matrix of hydrogen bonds.…”

Section: Viscositymentioning

confidence: 99%

“…The denaturation temperature of collagen is an irreversible process where the triple helix structure is lost due to the rupture of hydrogen bonds (Rochdi, Foucat, & Renou, 2000). The typical denaturation temperature of collagen is around 40°C; however, this temperature value varies from species to species, and the denaturation temperature of fish collagen is lower than that of collagen extracted from mammals (Huang et al, 2011;Yan et al, 2008;Zhang et al, 2007).…”

Section: Thermal Propertiesmentioning

confidence: 99%

“…This fibrous protein can be obtained from different sources and it is the main component of skins, bones and tendons (Ahmad & Benjakul, 2010;Delvin, 2004;Huang, Shiau, Chen, & Huang, 2011;Jongjareonrak, Benjakul, Visessanguan, Nagai, & Tanaka, 2005;Yan, Li, Zhao, & Qin, 2012). At least 29 different types of collagen with a singular amino acid sequence, structure and function have been identified (Li et al, 2013;Liu, Liang, Regenstein, & Zhou, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Collagen is a biopolymer composed of two alpha-1 chains and one alpha-2 chain. It is composed of a third part of glycine, and it does not contain tryptophan and lower amounts of tyrosine and histidine (Huang et al, 2011;Kittiphattanabawon, Benjakul, Visessanguan, Nagai, & Tanaka, 2005;Muyonga, Cole, & Duodu, 2004;Schrieber & Gareis, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Extraction and characterization of collagen from rabbit skin: partial characterization

Martínez-Ortiz

Hernández-Fuentes

Pimentel-González

et al. 2014

CyTA - Journal of Food

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(2015) Extraction and characterization of collagen from rabbit skin: partial characterization, CyTAJournal of Food, 13:2, 253-258, DOI: 10.1080/19476337.2014 To link to this article: https://doi.org/10. 1080/19476337.2014 Extraction and characterization of collagen were carried out in rabbit skins as a new alternative for collagen type I. Acetic acid and pepsin were for the extraction of soluble and insoluble collagen, respectively. The enzymatic treatment yielded higher amount of collagen (71%). The average pH value was 6.3, no matter what the method of extraction was. Denaturation temperature of collagen was found at 36°C approximately in two different techniques: Rheometer and Differential Scanning Calorimetry (DSC). Despite the amount of collagen in solution was low, its viscosity was high because of the hydrodynamic behaviour of collagen molecules. Sodium dodecyl sulphate polyacrylamide gel electrophoresis results showed three different bands that reflected two alpha-chains and one beta-chain with molecular weights of 102, 118 and 220 kDa, respectively. Determination of hydroxyproline gave evidence that the extracted material was collagen. It was concluded that rabbit skin could be an alternative source for the extraction of collagen.Keywords: collagen; rabbit skin; extraction; thermal analysis Se realizó la extracción y caracterización de colágeno en pieles de conejo como una nueva alternativa para colágeno tipo I. Se usaron ácido acético y pepsina para la extracción de colágeno soluble e insoluble respectivamente. El tratamiento enzimático fue el que rindió alta cantidad de colágeno (71%). El pH promedio fue de 6,3 sin importar cuál fue el método de extracción. La temperatura de desnaturalización del colágeno se encontró a 36°C aproximadamente mediante dos diferentes técnicas: Reometría y DSC. A pesar de que la cantidad de colágeno en la solución fue bajo, su viscosidad fue alta debido a el comportamiento hidrodinámico de las moléculas. Los resultados de SDS-PAGE mostraron tres diferentes bandas en las que se reflejaron dos cadenas alfa y una cadena beta con pesos moleculares de 102, 118 and 220 kDa respectivamente. La determinación del contenido de hidroxiprolina dio evidencia de que el material extraído fue colágeno. Se concluyó que la piel de conejo podría ser una alternativa de materia prima para la extracción de colágeno.

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Section: Yielding Of Extractionssupporting

confidence: 89%

Section: Viscositymentioning

confidence: 99%

Section: Thermal Propertiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Extraction and characterization of collagen from rabbit skin: partial characterization

Martínez-Ortiz

Hernández-Fuentes

Pimentel-González

et al. 2014

CyTA - Journal of Food

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Processing and Industrial Aspects of Fish‐scale Collagen: A Biomaterials Perspective

Dhara

Datta

Pal

et al. 2013

Marine Proteins and Peptides

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Preparation, characterization, and potential biomedical application of composite sponges based on collagen from silver carp skin

Chen

Huang

Guo

et al. 2014

J of Applied Polymer Sci

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To reuse fish processing waste for biomedical materials, collagen (Col) was extracted from silver carp skin, and Col–chitosan (Ch) composite sponges were prepared by a freeze‐drying method. The atomic force microscopy and electrophoresis results suggest the Col might have been type I. To obtain the optimum conditions for the manufacture of the Col–Ch sponges, the characteristics of sponges composed of different ratios of Col to Ch with different crosslinkers were evaluated. Scanning electron microscopy showed that the sponges had an interconnected network structure with porosity. Infrared spectroscopy demonstrated that intermolecular crosslinkages between Col and Ch occurred. The swelling measurements implied that all of the sponges could bind an 18‐ to 36‐fold amount of distilled water and still maintain their form and stability. When the ratio of Col to Ch was higher than 1:0.25, the swelling and degradation rate decreased with increasing Ch. Cell proliferation, hemolysis, and hemostasis assay indicated that the sponges exhibited noncytotoxicity, biocompatibility, nonhemolysis, and hemostatic efficacy. Overall, we concluded that the optimal ratio of Col and Ch for the sponges was 1:0.25, and glutaraldehyde crosslinking was more suitable than 1‐ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide hydrochloride. These results demonstrate the potential application of silver carp skin Col–Ch sponges for tissue engineering and wound dressing in non‐weight‐bearing tissue. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40998.

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Isolation and characterization of acid and pepsin-solubilized collagens from the skin of balloon fish (Diodon holocanthus)

Cited by 113 publications

References 29 publications

Extraction and characterization of collagen from rabbit skin: partial characterization

Extraction and characterization of collagen from rabbit skin: partial characterization

Processing and Industrial Aspects of Fish‐scale Collagen: A Biomaterials Perspective

Preparation, characterization, and potential biomedical application of composite sponges based on collagen from silver carp skin

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