Preparation, characterization, and potential biomedical application of composite sponges based on collagen from silver carp skin

Abstract

To reuse fish processing waste for biomedical materials, collagen (Col) was extracted from silver carp skin, and Col–chitosan (Ch) composite sponges were prepared by a freeze‐drying method. The atomic force microscopy and electrophoresis results suggest the Col might have been type I. To obtain the optimum conditions for the manufacture of the Col–Ch sponges, the characteristics of sponges composed of different ratios of Col to Ch with different crosslinkers were evaluated. Scanning electron microscopy showed that the sponges had an interconnected network structure with porosity. Infrared spectroscopy demonstrated that intermolecular crosslinkages between Col and Ch occurred. The swelling measurements implied that all of the sponges could bind an 18‐ to 36‐fold amount of distilled water and still maintain their form and stability. When the ratio of Col to Ch was higher than 1:0.25, the swelling and degradation rate decreased with increasing Ch. Cell proliferation, hemolysis, and hemostasis assay indicated that the sponges exhibited noncytotoxicity, biocompatibility, nonhemolysis, and hemostatic efficacy. Overall, we concluded that the optimal ratio of Col and Ch for the sponges was 1:0.25, and glutaraldehyde crosslinking was more suitable than 1‐ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide hydrochloride. These results demonstrate the potential application of silver carp skin Col–Ch sponges for tissue engineering and wound dressing in non‐weight‐bearing tissue. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40998.

Collagen/chitosan film containing biotinylated glycol chitosan nanoparticles for localized drug delivery

“…Besides, the drug films with nanoparticles possessed a looser aperture structure, which could provide a shortcut for the solutions to enter the internal film, also accounted for the higher swelling fold. showed a relatively higher degradation rate than that of Col1Ch0.25, which was consistent with the result of Col/Ch film without nanoparticles [20]. The reason might be that the higher swelling fold and lower crosslinking degree of films resulted in the higher degradation rate [28].…”

“…In our previous study, the cell viability of Col/Ch films without drug-loaded nanoparticles was above 90% even after 5 days of culturing, which implied the non-cytotoxicity of the films against the L02 cells [20]. As shown in Fig.7, the inhibition ratios of all drug films were above 85%, indicating that all the films had a high antitumor efficacy against HepG2 cells.…”

“…Previously, our group has isolated the collagen from silver carp skin, and prepared fish collagen/chitosan (Col/Ch) composite sponges [19,20]. The results showed that the presence of chitosan improved the biostability of collagen sponges, and the composite sponges exhibited noncytotoxicity, biocompatibility, and nonhemolysis.…”

A novel human-like collagen hemostatic sponge with uniform morphology, good biodegradability and biocompatibility

“…Here, two animal models were used to estimate the hemostasis efficacy: a rabbit ear artery and liver model, with the consideration that the liver is the most representative and important organ with its extremely abundant blood supply and it is sensitive to severely traumatic hemorrhaging. 31…”

Injectable negatively charged gelatin microsphere-based gels as hemostatic agents for intracavitary and deep wound bleeding in surgery

“…With the consideration that the liver is the most representative and important organ with extremely abundant blood supply, and it is sensitive to severely traumatic hemorrhaging. 31 The homeostasis time of all samples is shown in Figure 8 (Figure 8(d)), re-bleeding was not observed after the redundant sample had been removed from the hemorrhagic spot.…”