Extraction and characterization of collagen from rabbit skin: partial characterization

Abstract

(2015) Extraction and characterization of collagen from rabbit skin: partial characterization, CyTAJournal of Food, 13:2, 253-258, DOI: 10.1080/19476337.2014 To link to this article: https://doi.org/10. 1080/19476337.2014 Extraction and characterization of collagen were carried out in rabbit skins as a new alternative for collagen type I. Acetic acid and pepsin were for the extraction of soluble and insoluble collagen, respectively. The enzymatic treatment yielded higher amount of collagen (71%). The average pH value was 6.3, no matter what the method of extraction was. Denaturation temperature of collagen was found at 36°C approximately in two different techniques: Rheometer and Differential Scanning Calorimetry (DSC). Despite the amount of collagen in solution was low, its viscosity was high because of the hydrodynamic behaviour of collagen molecules. Sodium dodecyl sulphate polyacrylamide gel electrophoresis results showed three different bands that reflected two alpha-chains and one beta-chain with molecular weights of 102, 118 and 220 kDa, respectively. Determination of hydroxyproline gave evidence that the extracted material was collagen. It was concluded that rabbit skin could be an alternative source for the extraction of collagen.Keywords: collagen; rabbit skin; extraction; thermal analysis Se realizó la extracción y caracterización de colágeno en pieles de conejo como una nueva alternativa para colágeno tipo I. Se usaron ácido acético y pepsina para la extracción de colágeno soluble e insoluble respectivamente. El tratamiento enzimático fue el que rindió alta cantidad de colágeno (71%). El pH promedio fue de 6,3 sin importar cuál fue el método de extracción. La temperatura de desnaturalización del colágeno se encontró a 36°C aproximadamente mediante dos diferentes técnicas: Reometría y DSC. A pesar de que la cantidad de colágeno en la solución fue bajo, su viscosidad fue alta debido a el comportamiento hidrodinámico de las moléculas. Los resultados de SDS-PAGE mostraron tres diferentes bandas en las que se reflejaron dos cadenas alfa y una cadena beta con pesos moleculares de 102, 118 and 220 kDa respectivamente. La determinación del contenido de hidroxiprolina dio evidencia de que el material extraído fue colágeno. Se concluyó que la piel de conejo podría ser una alternativa de materia prima para la extracción de colágeno.

A Green Approach towards Native Collagen Scaffolds: Environmental and Physicochemical Assessment

“…Regarding collagen extraction, it is worth noting that there are several methods reported, which include the pretreatment of raw materials with chemicals, such as sodium hydroxide, butyl alcohol, and acids, or enzymes, such as pepsin and trypsin [30]. In contrast, only sodium hydroxide and mechanical pretreatments were used in this work, reducing the use of chemicals and bringing environmental and economic benefits, since lower amounts of resources (materials, energy, time) were employed in comparison to those works that use acid and enzymatic treatments [7,8]. In the analysis, the use of acetic acid (0.5 M) in a ratio of 1:20 (w/v) in the scaffolds preparation process, as well as the distilled water production procedure, were also considered.…”

“…However, not only raw materials but also the processes followed to extract valuable polymers from those by-products are essential in order to move towards more sustainable productive systems. In this regard, the most common methods used to extract collagen include acid and enzymatic treatments, which are harsh and long processes that involve the use of chemicals and energy [7,8]. It is worth noting that those processes have been avoided in this work.…”

Hydrolysed Collagen from Sheepskins as a Source of Functional Peptides with Antioxidant Activity

“…The structure of collagen is a triple helix formed for 3 α chains Gly-X-Y, where X is proline, Y is mainly hydroxyproline, and the triple helix is stabilized for hydrogen bonds with continuous repetition of the Gly-X-Y depending on the collagen type [3]. Collagen can be extracted from the skins, bones, tendons and cartilages of pigs [4], cows [5], marine organisms [6,7,8] and rabbits [9]. Hydrolysed collagen (HC) refers to a group of peptides that results from the proteolysis of native collagen type 1; its molecular weight (Mw) varies from 0.3 to 8 KDa [10].…”

Optimization of Conditions for Extraction of Pepsin-Soluble Collagen from Indonesian Local “Kacang” Goatskin by Response Surface Methodology

“…Catla skin which extracted using pepsin has yield 69.53% with a time of hydrolysis was 48 h (Pal et al, 2015). PSC yield from rabbit skin with enzymatic treatment for 48 h was 71% (Pimentel-gonzález et al, 2015).…”