Isolation and characterization of a granulosis virus from the tomato moth, Lacanobia oleracea, and its potential as a control agent

Interactions Between the ParasitoidMeteorus gyrator(Hymenoptera: Braconidae) and a Granulovirus inLacanobia oleracea(Lepidoptera: Noctuidae)

“…The moth Lacanobia oleracea (L.) is an economically important pest of glasshouse tomatoes (Lycopersicum spp.). Its granulovirus (LoGV) has the ability to kill all larval stages and also causes deleterious effects to the survivors of virus challenge (Crook et al 1982, Matthews et al 2002. However, the use of LoGV is limited because very high doses are required to kill the larger and most damaging (Marris and Edwards 1995), larval stages (Matthews et al 2002).…”

Sequence and in vivo transcription of Lacanobia oleracea granulovirus egt.

“…Purified occlusion bodies of a Scottish isolate of LoGV (Crook et al, 1982) were kindly provided by Norman Crook (Horticulture Research International, Wellesbourne, UK). LoGV was propagated in fifth-instar L. oleracea larvae from a colony established at the Central Science Laboratory in 1991 (Corbitt et al, 1996).…”

“…The virus used for the DNA cloning experiments was derived from an individual larval cadaver among a group of insects infected with a 30 % mortality dose of LoGV. It is therefore likely (but, statistically, by no means certain) to comprise a single genotype (Huber & Hughes, 1984) ; its genomic DNA restriction profiles were free of visible submolar bands in ethidium bromide staining (Smith & Crook, 1988) and were indistinguishable from those of wild-type LoGV (Crook et al, 1982). Stocks of this virus are designated LoGV-S1 and constitute the genotype referred to throughout this work as LoGV.…”

“…We are using a Scottish isolate of a GV pathogenic for the tomato moth, Lacanobia oleracea (Crook et al, 1982). This virus is designated LoGV, and two of its genes are under study : granulin and egt.…”

Restriction Enzyme Analysis of Granulosis Viruses Isolated from Artogeia rapae and Pieris brassicae

“…Although a number of variants have been identified for other baculoviruses such as Autographa californica MNPV (Smith & Summers, 1979), Mamestra brassicae MNPV (Vlak & Gr6ner, 1980;Wiegers & Vlak, 1984), Lacanobia oleracea GV (Crook et al, 1982), Heliothis SNPV and MNPV (Gettig & McCarthy, 1982;Williams & Payne, 1984), Spodopterafrugiperda MNPV (Maruniak et al, 1984) and Cydia pomonelta GV (Crook et al, 1985) and small differences in the infectivities of different isolates have been found (Williams & Payne, 1984), there are no reports of very large changes in the infectivity for a host insect especially where the infectivity for another host remains unchanged. The ArGV isolates therefore provide a unique system for studying the effect of genotypic differences on virulence and host range.…”