We have determined the nucleotide sequence and located the major in vivo transcript termini of the Lacanobia oleracea granulovirus (LoGV) egt gene. The open reading frame encodes a 460-amino acid polypeptide having extensive sequence similarity to ten nucleopolyhedrovirus (NPV) ecdysteroid UDPglucosyltransferase (EGT) proteins ; the degree of similarity is particularly high within several previously identified EGT ' domains ', and eight invariant amino acid residues are conserved. A phylogenetic tree, constructed by the neighbour joining method, showed LoGV EGT to be the most highly diverged of the eleven baculovirus sequences compared. Database searching revealed that part of a published DNA sequence from Cryptophlebia leucotreta granulovirus appears to encode the N-terminal
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