Restriction Enzyme Analysis of Granulosis Viruses Isolated from Artogeia rapae and Pieris brassicae

Comprehensive physical map of the Cydia pomonella granulovirus genome and sequence analysis of the granulin gene region.

“…The two ORFs in this sequence initiated between 124 and 125 kbp and between 0 and 1 kbp and were thus named ORF124R and ORF0R. ORF0R was assumed to be the granulin gene as it showed high homology to previously sequenced granulin genes [from TnGV (Akiyoshi et al, 1985), Cryptophlebia leucotreta GV (ClGV) (Jehle & Backhaus, 1994) and Pieris brassicae GV (Chakerian et al, 1985), referred to as ArGV since it is a variant of Artogeia rapae GV (Crook, 1986)]. For a comparison of the homology of these granulin genes, see Table 1 in Jehle & Backhaus (1994).…”

Restriction endonuclease analysis and mapping of the genomes of granulosis viruses isolated from Xestia c-nigrum and five other noctuid species

“…To evaluate this assumption we conducted restriction endonuclease analysis of all the isolates, and here we report our results which show that all of the isolates are in fact the same GV with minor variations between isolates from different host species. Genomic variants of GVs have been detected in isolates from the same host species, such as Cydia pomonella (Crook et al, 1985) and Artogeia (= Pieris) rapae (Crook, 1986;Smith & Crook, 1988a), and from closely related host species, such as Choristoneura spp. (Arif et al, 1986).…”

Physical Maps of the Genomes of Four Variants of Artogeia rapae Granulosis Virus

“…Despite the differences between these isolates, they are more similar to each other than any of them is to the Canadian isolate (ArGV5 in Crook, 1986) mapped by Dwyer & Granados (1987). This isolate shows a number of restriction site differences for each of the enzymes (EcoRI, HindlII, BamHI and KpnI) which are shown on both maps and in addition there are probably a number of small insertions and deletions which would be detected only by a direct comparison of double digest profiles, since the size differences are sometimes too small to show up on single digest profiles.…”

“…1 and also for digests with XhoI, which gave very similar profiles for all four virus genotypes (Crook, 1986), are listed in Table 1. Calculation of mean total genome sizes gave values of 110.1 kbp (ArGV1), 110.0 kbp (ArGV2), 109.4 kbp (ArGV3) and 110.0 kbp (ArGV4).…”

“…The granulin gene from one of these isolates (ArGV1) has been sequenced previously and this was used to orientate the maps-and locate their zero point. The origins of ArGV1 to ArGV4 have been described by Crook (1986). Virus was routinely propagated in fourth instar A. rapae and purified as previously described (Crook & Payne, 1980).…”