Isolation and characterization of a novel antihyperglycemic protein from the fruits of Momordica cymbalaria

Rajasekhar, Maddirela Dilip; Badri, Kameswara Rao; Kumar, K.; Kassetti, Ramesh Babu; Fatima, Shaik Sameena; Kumar, Mekala Thur Sampath; Rao, Chippada Appa

doi:10.1016/j.jep.2009.12.025

Cited by 24 publications

(24 citation statements)

References 19 publications

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“…Diabetes mellitus was induced in overnight-fasted rats by a single intraperitoneal injection of freshly prepared STZ (50 mg/kg, dissolved in 0.1 M cold citrate buffer, pH 4.5) as described by our group earlier [12, 13]. Diabetes was identified by polydipsia, polyuria and by measuring fasting blood glucose (FBG) levels.…”

Section: Methodsmentioning

confidence: 99%

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Anti-Diabetic Potential of the Leaves of Anisomeles malabarica in Streptozotocin Induced Diabetic Rats

Kotha

Badri

Nagalapuram

et al. 2017

Cell Physiol Biochem

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Background/Aims: Diabetes mellitus is a pandemic metabolic disorder that is affecting a majority of populations in recent years. There is a requirement for new drugs that are safer and cheaper due to the side effects associated with the available medications. Methods: We investigated the anti-diabetic activity of leaves of Anisomeles malabarica following bioactivity guided fractionation. The different solvent (hexane, ethyl acetate, methanol and water) extracts of A. malabarica leaves were used in acute treatment studies to evaluate and identify the active fraction. The ethyl acetate extract was subjected to further fractionation using silica gel column chromatography and the compounds were identified by LC-SRM/MS and GC-MS. Additional chronic treatment studies were carried out using this active fraction (AMAF) for 30 days in experimental diabetic rats. Fasting blood glucose (FBG), glycosylated hemoglobin (HbA1c), plasma insulin levels and glucose tolerance were measured along with insulin resistance/sensitivity indicators (HOMA-IR, HOMA-β and QUICKI) to assess the beneficial effects of A. malabarica in the management of diabetes mellitus. Results: Among the different solvent extracts tested, ethyl acetate extract showed maximum (66%) anti-hyperglycemic activity. The hexane and ethyl acetate (1: 1) fraction that has maximum anti-diabetic activity was identified as active fraction of A. malabarica (AMAF). The FBG, HbA1c, plasma insulin levels and insulin sensitivity/resistance indicators such as glucose tolerance, HOMA-IR, HOMA-β and QUICKI were significantly improved to near normal in diabetic rats treated with AMAF. Further, we identified key flavonoids and fatty acids as the anti-diabetic active principles from the AMAF of A. malabarica leaves. Conclusion: The results of our study suggest that Anisomeles malabarica has potential anti-diabetic activity in STZ induced diabetic rats.

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Section: Methodsmentioning

confidence: 99%

“…Estimation of blood glucose was carried out using dextrostix with Accucheck glucometer (Glucose oxidase method) [12, 13]. HbA1c was estimated by the method of Eross et al [14].…”

Section: Methodsmentioning

confidence: 99%

Anti-Diabetic Potential of the Leaves of Anisomeles malabarica in Streptozotocin Induced Diabetic Rats

Kotha

Badri

Nagalapuram

et al. 2017

Cell Physiol Biochem

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“…Increased weights of rats with the treatment of Mcy protein also confirms the elevated insulin levels in Mcy treated diabetic group. However, stimulation of remnant pancreatic β cells by Mcy protein and insulin mimicking activity of Mcy protein due to possible structural similarity with insulin [10] cannot be ignored. Previously similar studies using protein extracts from the fruits of Momordica charantia showed insulin secretagogue and insulinomimetic activities in STZ induced diabetic rats [38].…”

Section: Discussionmentioning

confidence: 99%

“…Fruits of M. cymbalaria were collected [9,10] and Mcy protein was purified from the aqueous extract of fruits of M. cymbalaria as previously described [10]. In the current study Mcy protein was used at a dose of 2.5mg/kg b.w.…”

Section: Methodsmentioning

confidence: 99%

“…The animals were maintained in standard animal house conditions with ad libitum access to food and water under a 12-hour light dark cycle prior to treatment. DM was induced in overnight fasted rats by single intravenous injection of freshly prepared ice cold Streptozotocin (STZ) (50mg/kg body weight) in citrate buffer (pH 4.5)[10]. Diabetes was confirmed by measuring fasting blood glucose concentrations.…”

Section: Methodsmentioning

confidence: 99%

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Antihyperlipidemic and Biochemical Activities of Mcy Protein in Streptozotocin Induced Diabetic Rats

Marella

Maddirela

Badri

et al. 2015

Cell Physiol Biochem

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4,312

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Background: This study was aimed to evaluate the protective effects of a novel anti-hyperglycemic “Mcy protein” isolated from the fruits of Momordica cymbalaria in streptozotocin induced- diabetes rat model. Materials and Methods: Wild type and Streptozotocin induced diabetic male wistar albino rats were either treated with single intraperitoneal injection of 2.5 mg Mcy protein/kg body weight or acetate buffer daily for 30 days. Fasting blood glucose and, serum and tissue lipid levels were measured along with biochemical analysis for hepatic and renal function tests. Results: Mcy protein significantly reduced the fasting blood glucose and, serum as well as tissue lipid levels (p<0.05), besides normalizing the levels of liver and kidney function markers in the treated diabetic rats when compared to the diabetic controls. Our studies also showed the pancreatic islet regeneration in Mcy treated rats. Conclusion: Mcy protein can alleviate hyperlipidemia and help manage diabetes by stimulating insulin secretion without evident toxic effects on liver and kidney.

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Plant-Derived Bioactive Peptides: A Treatment to Cure Diabetes

Patil

Goswami

Kalia

et al. 2019

Int J Pept Res Ther

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Recent advances in analytical techniques have opened new opportunities for plant-based drug discovery in the field of peptide and proteins. Enzymatic hydrolysis of plant parent proteins forms bioactive peptides which are explored in the treatment of various diseases. In this review, we will discuss the identified plant-based bioactive proteins and peptides and the in vitro, in vivo results for the treatment of diabetes. Extraction, isolation, characterization and commercial utilization of plant proteins is a challenge for the pharmaceutical industry as plants contain several interfering secondary metabolites. The market of peptide drugs for the treatment of diabetes is growing at a fast rate. Plant-based bioactive peptides might open up new opportunities to discover economic lead for the management of various diseases.

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Isolation and characterization of a novel antihyperglycemic protein from the fruits of Momordica cymbalaria

Cited by 24 publications

References 19 publications

Anti-Diabetic Potential of the Leaves of Anisomeles malabarica in Streptozotocin Induced Diabetic Rats

Anti-Diabetic Potential of the Leaves of Anisomeles malabarica in Streptozotocin Induced Diabetic Rats

Antihyperlipidemic and Biochemical Activities of Mcy Protein in Streptozotocin Induced Diabetic Rats

Plant-Derived Bioactive Peptides: A Treatment to Cure Diabetes

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