Involvement of Opioid System, TRPM8, and ASIC Receptors in Antinociceptive Effect of Arrabidaea brachypoda (DC) Bureau

Abstract

Arrabidaea brachypoda (DC) Bureau is a medicinal plant found in Brazil. Known as “cipó-una”, it is popularly used as a natural therapeutic agent against pain and inflammation. This study evaluated the chemical composition and antinociceptive activity of the dichloromethane fraction from the roots of A. brachypoda (DEAB) and its mechanism of action. The chemical composition was characterized by high-performance liquid chromatography, and this fraction is composed only of dimeric flavonoids. The antinociceptive effect was evaluated in formalin and hot plate tests after oral administration (10–100 mg/kg) in male Swiss mice. We also investigated the involvement of TRPV1 (transient receptor potential vanilloid 1), TRPA1 (transient receptor potential ankyrin 1), TRPM8 (transient receptor potential melastatin 8), and ASIC (acid-sensing ion channel), as well as the opioidergic, glutamatergic, and supraspinal pathways. Moreover, the nociceptive response was reduced (30 mg/kg) in the early and late phase of the formalin test. DEAB activity appears to involve the opioid system, TRPM8, and ASIC receptors, clearly showing that the DEAB alleviates acute pain in mice and suggesting the involvement of the TRPM8 and ASIC receptors and the opioid system in acute pain relief.

Anticancer activities of Brachydin C in human prostate tumor cells (DU145) grown in 2D and 3D models: Stimulation of cell death and downregulation of metalloproteinases in spheroids

“…The chemical structure of brachydins is composed of four rings (A, B, C, and D) whose structures differ with regard to the substituent group (R) of the C ring: brachydin A (BrA) has a hydroxyl group, brachydin B (BrB) a methoxy group, and brachydin C (BrC) has no substituent on the C ring (da Rocha et al, 2014; Figure 1a). With regard to their biological actions, the brachydins showed the following: (i) an antinociceptive effect in vivo (Rodrigues et al, 2017); (ii) anti‐ Trypanosoma cruzi activity in vivo and in vitro (da Rocha et al, 2014); (iii) anti‐ Leishmania sp. activity in vitro (de Sousa Andrade et al, 2020); and (iv) antimicrobial activity against Staphylococcus aureus (de Sousa Andrade et al, 2020).…”

Avaliação do extrato floral de Fridericia platyphylla (Cham.) L. G. Lohmann sobre Candida krusei, C. tropicalis, C. guilliermondii e C. albicans

“…Em outros estudos, avaliando a fração diclorometânica do extrato hidroetanólico da raiz, não induziu atividade estrogênica, no entanto, apresentou boa eficiência como gastroprotetor , antinociceptivo e antiinflamatório (Rocha et al, 2011) e no alívio de dores agudas em camundongos estimulados (Rodrigues et al, 2017;Nunes et al, 2020). No entanto, ainda pouco se conhece sobre a atividade antifúngica utilizando extratos e suas frações e subfrações de F. platyphylla, e como discutido anteriormente, são escasos os estudos com espécies potencialmente perigosas para o homem.…”

Hydroalcoholic extract of leaves of Arrabidaea brachypoda (DC.) Bureau present antispasmodic activity mediated through calcium influx blockage

“…) LG Lohmann), belonging to the Bignoniaceae Family and to the genus Arrabidaea, it is a native bush of the Brazilian Cerrado, with 1.0-2.0 m height, popularly known as "cervejinha do campo", and used in the southeast and northeast of Brazil for the treatment of kidney stones and painful joints (arthritis). This species has been studied from a chemical and pharmacological point of view (Yanagizawa & Maimoni-Rodella, 2007;Rocha et al, 2011 andRodrigues et al, 2017;Serpeloni et al, 2020;Bertanha et al, 2020).…”