Hydroalcoholic extract of leaves of Arrabidaea brachypoda (DC.) Bureau present antispasmodic activity mediated through calcium influx blockage

Abstract

Aim: Since other species of the Bignoniaceae Family presented of antispasmodic activity, it was decided, by chemotaxonomic criterion, to determine the antispasmodic activity of the leaves of Arrabidaea brachypoda. Methodology: the segments of the rat jejunum were suspended in glass vats containing specific saline solution, at an appropriate temperature, and after stabilization period, were stimulated by a contractile agent to observe the inhibitory or relaxing effect of EH-FAB. Results: EH-FAB showed the presence of 10 compounds, mainly rutin and it has an antispasmodic activity as it inhibits the phasic component and relaxes the tonic component of the contraction in isolated rat jejunum. To assess the mechanism of antispasmodic action, cumulative curves to the CCh were performed in which a non-competitive antagonism was observed, due to a displacement of the control curve to the right and reduction of the maximum contraction effect (E max). Afterward, the participation of the calcium and/or potassium channels was evaluated by increasing the extracellular potassium, and it was observed that the EH-FAB relaxed the rat jejunum, suggesting the participation of the Ca 2+ channels. To corroborate that hypothesis, the EH-FAB was tested against cumulative curves to Ca 2+ in a free depolarizing solution of Ca 2+ , and it was observed that there was a shift of the curve to the right with a reduction in E max. Conclusions: EH-FAB presents antispasmodic activity in isolated rat jejunum and it is suggested to block the influx of Ca 2+ through voltage-gated calcium channels, signaling the therapeutic potential for the treatment of colic and/or diarrhea.

Protective Effects of the Hydroethanolic Extract of Fridericia chica on Undifferentiated Human Neuroblastoma Cells Exposed to α-Zearalenol (α-ZEL) and β-Zearalenol (β-ZEL)

“…Fridericia chica (synonym Arrabidaea chica) leaves are usually employed as a red dye, in fact, it has traditionally been used by Sinu artisans of the Colombian Caribbean to make the vueltiao hat [2]. The red color obtained from F. chica comes from anthocyanidins, a class of phenolic compounds with known antioxidant properties [3], and extracts from the plant have shown pharmacological activities linked to beneficial health effects, including anti-inflammatory [4], antiproliferative [5], wound healing [6], antispasmodic [7], photoprotective [8], and leishmanicidal [9] activities. Many of these properties are attributed to different flavonoid compounds reported in F. chica, such as isoscutellarein, 6-hydroxyluteolin, hispidulin, scutellarein, luteolin, apigenin, and hispidulin [10], this last a potential compound for neuroinflammation inhibition [11].…”

A Review of the Phytochemistry and Pharmacological Properties of the Genus Arrabidaea

“…EH-FAB inhibited the contraction and relaxation of isolated rat jejunum in a significant and concentration-dependent manner. The authors also observed the significant relaxation of the rat jejunum when it was precontracted by high concentrations of extracellular potassium, demonstrating the participation of the potassium channel in the relaxation mechanism of EH-FAB, and this further shifted the CaCl 2 curve to the right, with a decrease in the maximal effect that is characteristic of the CaV inhibition of the calcium influx [31].…”

“…A phenylethanoid glycoside, namely conadroside (24) was isolated from leaves of A. brachypoda by Bertanha et al [30]. The chemical characterization of the leaves extract revealed the presence of flavonoids apigenin (25), luteolin (26), isoquercitrin (27), rutin (28); furthermore, four chalcones were isolated from the flowers, namely 4 -hydroxy-3-4-dimethoxy-chalcone (29), 3 -hydroxy-3acetate, 4-methoxy-chalcones (30), 3 ,4 -dihydroxy, 3,4,4 -trimethoxy-chalcone (31), and 3,4-dimethoxy-chalcone (32) [31][32][33]. Bio-Pharmacological Properties of A. brachypoda This is one of the species most studied by our research group.…”

Avaliação do extrato floral de Fridericia platyphylla (Cham.) L. G. Lohmann sobre Candida krusei, C. tropicalis, C. guilliermondii e C. albicans

“…Estudos rescente com F. platyphylla descrevem sobre a presença de óleo essencial nas flores (Menezes Filho, 2020), flavonol rutina nas folhas (Lima, 2018), cirsimaritina, cirsiliol, hispidulina e 3 ',4'-hiidroxi-5,6,7-trimetoxiflavona (Alcerito et al, 2002;Patel;Patel, 2017), apigenina, luteolina, hispidulina, cirsiliol, 7-metoxipigenina-6-C-hexose, 7-metoxiliteolina-6-C-hexose, isoquercetina, apigenina-6-C-hexose, 8-C-hexose, rutina e arrabidosídeo A (Monteiro et al, 2020), e dois importantes flavonoides braquidinas A, B e C isolados do extrato hidroetanólico das raízes (Rocha et al, 2014;Nunes et al, 2020).…”