Investigation of the bioactivity and biocompatibility of different glass interfaces with hydroxyapatite, fluorohydroxyapatite and 58S bioactive glass

Abstract

The current review investigates the bioactivity of different glass interfaces created on thin glass cover slips as substrates. The interfaces studied are plain glass, functionalized glass using 0.5 M and 5 M of sodium hydroxide (NaOH) for 24 hrs, and glass coated with bioactive 58S Bioglass (58S). A biomimetic method, involving the exposure of the three interfaces to 1.5 times simulated body fluid (SBF) tests the bioactivity of the interfaces via creation of layer of Hydroxyapatite (HA). Fluorinated SBF will precipitate fluorine doped HA (FHA) on a bioactive interface. Higher concentration of 1.5 times of SBF used in this study intended to accelerate the formation of HA and FHA layer over the substrate. HA and FHA is found to be precipitated on the thinly coated 58S. This paper, study also the thin film coatings of three forms of bioceramics - bioactive 58S, HA and FHA. The study, also proposes to draw a relation between the morphology of HA particles with duration of exposure to SBF, the effects of fluorine on the morphology and the cell interaction with bioactive 58S, HA and FHA interfaces using pre-differentiated osteoblastic MC3T3 cells. The analysis of cells in this study is confined to three parameters that include the attachment, proliferation and viability of cells. Tests employed for the analysis of the thin film coating of HA and FHA is restricted to qualitative X-Ray Diffraction and quantitative Field Emission Scanning Electron Microscope. Other mechanical tests such as shear test are not used to test the mechanical properties of this thin layer, due to the fact that the thin film is too thin for such analysis.

Avaliação histológica da biocompatibilidade de otólitos Cynoscion acoupa em ratos

“…Every material for biological use is supposed to be biocompatible (HAN et al, 2007;MORETTI-NETO et al, 2008). In this study, the biocompatibility of otoliths preparation materials was assessed by examining their inflammatory reactions in 3, 7 and 30 days after subcutaneous implantation in the dorsal area of rats.…”

“…In this study, the biocompatibility of otoliths preparation materials was assessed by examining their inflammatory reactions in 3, 7 and 30 days after subcutaneous implantation in the dorsal area of rats. This type of subcutaneous implant is widely used and easily reproduced (HAN et al, 2007;MORETTI-NETO et al, 2008;MAIZATO et al, 2008).…”

“…However, both organic and inorganic biomaterials are characterized by biocompatibility and atoxicity, in addition to mechanical resistance, elasticity, and chemical and biological stability. Therefore, new materials demand research to evaluate their properties and commercial viability (HAN et al, 2007;MORETTI--NETO et al, 2008).…”

Evaluation of osteogenesis and angiogenesis of icariin loaded on micro/nano hybrid structured hydroxyapatite granules as a local drug delivery system for femoral defect repair

“…10,11 Therefore, porous scaffolds and granules of HAp bioceramics have been developed and widely used in clinical bone regenerations. 10,11 Therefore, porous scaffolds and granules of HAp bioceramics have been developed and widely used in clinical bone regenerations.…”

In VitroEnhancement of SAOS-2 Cell Calcified Matrix Deposition onto Radio Frequency Magnetron Sputtered Bioglass-Coated Titanium Scaffolds

“…[70][71][72] Further, a stable bioactive glass made by sol-gel processing should favor the rapid formation of HA. 44,73 Bioactive glass deposition by the magnetron sputtering technique allows the formation of a film of controlled stoichiometry, showing good homogeneity and adhesive properties that should promote the formation of a HA layer which is, ultimately, functional for the osteointegration processes.…”