Investigation of Human Papillomavirus in Transitional Cell Carcinomas of the Urinary Bladder in South Africa

Sur, Monalisa; Cooper, K; Allard, U

doi:10.1080/00313020120034849

Cited by 8 publications

(12 citation statements)

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“…Until now, the role of HPV in the pathogenesis of bladder carcinomas has been extensively investigated with contradictory results showing a range of positivity rates between 0 and 81% [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26]. The highly variable incidence of HPV infection reported in bladder cancer was attributed to either geographical variations or some technical issues such as sampling problems, contamination or sensitivity of detection systems [16,23,31].…”

Section: Discussionmentioning

confidence: 98%

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Role of human papillomavirus in the development of urothelial carcinoma

et al. 2010

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It has been estimated that almost 10% of the worldwide cancer burden is linked to human papillomavirus (HPV) infection. Although the association between HPV and bladder carcinoma has been extensively investigated, data on the role of HPV in bladder carcinogenesis are controversial. The aim of the study was to assess the possible role of human papillomavirus in the development of urothelial bladder carcinomas. Formalin-fixed and paraffin-embedded archival tissue samples were used for DNA extraction. Seventy urothelial bladder carcinoma tissues were screened by nested-polymerase chain reaction (PCR) for HPV DNA with a control group of total 18 cervical tissues with invasive cervical carcinoma and cervical intraepithelial neoplasia III (CIN III). In the study group, we did not find HPV DNA positivity in any of the urothelial carcinomas. In the control group, 15 out of 18 (83.3%) samples were positive for the HPV DNA. These results indicated that there was no association between HPV infection and urothelial carcinomas.

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Section: Discussionmentioning

confidence: 98%

“…Until now, conflicting findings have been reported on association between HPV infection and bladder carcinoma [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26]. In the present study, we analyzed urothelial bladder tumor samples for HPV in order to clarify the controversial role of HPV in bladder cancers.…”

Section: Introductionmentioning

confidence: 87%

Role of human papillomavirus in the development of urothelial carcinoma

et al. 2010

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“…The association between HPV and bladder cancer has been explored in some studies in Africa. HPV was observed at a low percentage in South Africa (1.1%) [18] and no evidence of HPV infection was detected by morphological examination and PCR in any case of bladder carcinoma from Tunisian patients [7]. In contrast, a PCR-based study has revealed the detection of HPV DNA sequences in 49% cases of bladder cancer from Egyptian patients [19].…”

Section: Discussionmentioning

confidence: 88%

“…Some studies have investigated the association between HPV and bladder cancer in Africa. HPV was found to be rare in South Africa (1.1%) [18] and absent in Tunisia [7]. In contrast, a PCR-based study reported the detection of HPV DNA sequences in 49% cases of bladder cancer in Egypt [19].…”

Section: Introductionmentioning

confidence: 95%

Human papillomavirus detection in Moroccan patients with bladder cancer

Berrada

Albouzidi

Ameur

et al. 2013

J Infect Dev Ctries

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Introduction: Human papillomavirus (HPV) is associated with more human cancers than any other virus. Many studies have investigated the association between bladder cancer and HPV but the results remain controversial. The aim of the present study is to evaluate whether HPV have an etiological role in bladder carcinogenesis among Moroccan patients. Methodology: Forty-eight fresh biopsies (43 bladder tumors and 5 non-tumor samples) were collected for this purpose. Nested PCR with the consensus MY09/MY11 and GP5

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“…Many of the previous studies failed to find HPV in these tumours (9), whereas some others reported its detection in up-to 57% of cases (10).…”

Section: Introductionmentioning

confidence: 94%

Human papillomavirus is detected in transitional cell carcinoma arising in renal transplant recipients

et al. 2009

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Aims: We investigated the role of human papillomavirus (HPV) in the development of transitional cell carcinoma (TCC) arising in renal transplant recipients.Methods: Genomic DNA was extracted from 10 µm paraffin embedded sections of five TCCs arising in five renal transplant recipients using the QIAamp DNA mini kit according to the manufacturer's instructions. β-globin PCR was performed to test DNA adequacy. Samples were tested for the presence of HPV DNA by broad spectrum HPV PCR method using non-biotinylated SPF10 primers (SPF1A, SPF1B, SPF1C, SPF1D, SPF2B, SPF2D) which amplify a short 65 bp fragment. Positive bands were identified on a 3% gel. Positive samples underwent a second HPV PCR and were amplified using biotinylated SPF10 primer set, which amplifies the same 65 bp region of the L1 open reading frame. INNO-LiPA line probe assay was then performed to genotype the samples which uses a reverse hybridisation principle.Results::Four of five TCCs examined were positive for HPV. The high risk HPV16 was detected in three cases whereas in the fourth case an unclassifiable HPV genotype was present. In all DNA samples, β-globin amplification was successful.Conclusions: Our results indicate that HPV and in particular HPV16 may play an aetiological role in the development of TCC in renal transplant patients. Introduction:

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Investigation of Human Papillomavirus in Transitional Cell Carcinomas of the Urinary Bladder in South Africa

Cited by 8 publications

References 0 publications

Role of human papillomavirus in the development of urothelial carcinoma

Role of human papillomavirus in the development of urothelial carcinoma

Human papillomavirus detection in Moroccan patients with bladder cancer

Human papillomavirus is detected in transitional cell carcinoma arising in renal transplant recipients

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